Amphibian larvae and zinc sulphate: A suitable model to study the role of brain-derived neurotrophic factor (BDNF) in the neuronal turnover of the olfactory epithelium
The vertebrate olfactory system has fascinated neurobiologists over the last six decades because of its ability to replace its neurons and synaptic connections continuously throughout adult life, under both physiological and pathological conditions. Among the factors that are proposed to be involved...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0302766X_v336_n1_p1_Yovanovich http://hdl.handle.net/20.500.12110/paper_0302766X_v336_n1_p1_Yovanovich |
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paper:paper_0302766X_v336_n1_p1_Yovanovich2023-06-08T15:28:11Z Amphibian larvae and zinc sulphate: A suitable model to study the role of brain-derived neurotrophic factor (BDNF) in the neuronal turnover of the olfactory epithelium Yovanovich, Carola Anabel Mariana Jungblut, Lucas David Pozzi, Andrea Gabriela Paz, Dante Agustin Neurotrophins Olfactory neuron Regeneration Rhinella arenarum (bufo arenarum) (anura) brain derived neurotrophic factor zinc sulfate Amphibia animal animal embryo animal model article biological model cell proliferation drug effect evaluation growth, development and aging injury larva metabolism nerve cell nerve regeneration olfactory epithelium olfactory nerve physiology prenatal development randomization Amphibians Animals Brain-Derived Neurotrophic Factor Cell Proliferation Embryo, Nonmammalian Larva Models, Animal Models, Biological Nerve Regeneration Neurons Olfactory Mucosa Olfactory Nerve Random Allocation Zinc Sulfate The vertebrate olfactory system has fascinated neurobiologists over the last six decades because of its ability to replace its neurons and synaptic connections continuously throughout adult life, under both physiological and pathological conditions. Among the factors that are proposed to be involved in this regenerative potential, brain-derived neurotrophic factor (BDNF) is a candidate for having an important role in the neuronal turnover in the olfactory epithelium (OE) because of its well-documented neurogenic and trophic effects throughout the nervous system. The aim of the present study was to generate a suitable model to study the participation of BDNF in the recovery of the OE after injury in vivo. We developed an experimental design in which the OE of Rhinella arenarum tadpoles could be easily and selectively damaged by immersing the animals in ZnSO4 solutions of various concentrations for differing time periods. Image analysis of histological sections showed that different combinations of each of these conditions produced statistically different degrees of injury to the olfactory tissue. We also observed that the morphology of the OE was restored within a few days of recovery after ZnSO 4 treatment. Immunohistochemical analysis of BDNF was performed with an antiserum whose specificity was confirmed by Western blotting, and which showed drastic changes in the abundance and distribution pattern of this neurotrophin in the damaged olfactory system. Our results thus suggest that BDNF is involved in the regeneration of the OE of amphibian larvae, and that our approach is suitable for further investigations of this topic. © Springer-Verlag 2009. Fil:Yovanovich, C.A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jungblut, L.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pozzi, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paz, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0302766X_v336_n1_p1_Yovanovich http://hdl.handle.net/20.500.12110/paper_0302766X_v336_n1_p1_Yovanovich |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Neurotrophins Olfactory neuron Regeneration Rhinella arenarum (bufo arenarum) (anura) brain derived neurotrophic factor zinc sulfate Amphibia animal animal embryo animal model article biological model cell proliferation drug effect evaluation growth, development and aging injury larva metabolism nerve cell nerve regeneration olfactory epithelium olfactory nerve physiology prenatal development randomization Amphibians Animals Brain-Derived Neurotrophic Factor Cell Proliferation Embryo, Nonmammalian Larva Models, Animal Models, Biological Nerve Regeneration Neurons Olfactory Mucosa Olfactory Nerve Random Allocation Zinc Sulfate |
| spellingShingle |
Neurotrophins Olfactory neuron Regeneration Rhinella arenarum (bufo arenarum) (anura) brain derived neurotrophic factor zinc sulfate Amphibia animal animal embryo animal model article biological model cell proliferation drug effect evaluation growth, development and aging injury larva metabolism nerve cell nerve regeneration olfactory epithelium olfactory nerve physiology prenatal development randomization Amphibians Animals Brain-Derived Neurotrophic Factor Cell Proliferation Embryo, Nonmammalian Larva Models, Animal Models, Biological Nerve Regeneration Neurons Olfactory Mucosa Olfactory Nerve Random Allocation Zinc Sulfate Yovanovich, Carola Anabel Mariana Jungblut, Lucas David Pozzi, Andrea Gabriela Paz, Dante Agustin Amphibian larvae and zinc sulphate: A suitable model to study the role of brain-derived neurotrophic factor (BDNF) in the neuronal turnover of the olfactory epithelium |
| topic_facet |
Neurotrophins Olfactory neuron Regeneration Rhinella arenarum (bufo arenarum) (anura) brain derived neurotrophic factor zinc sulfate Amphibia animal animal embryo animal model article biological model cell proliferation drug effect evaluation growth, development and aging injury larva metabolism nerve cell nerve regeneration olfactory epithelium olfactory nerve physiology prenatal development randomization Amphibians Animals Brain-Derived Neurotrophic Factor Cell Proliferation Embryo, Nonmammalian Larva Models, Animal Models, Biological Nerve Regeneration Neurons Olfactory Mucosa Olfactory Nerve Random Allocation Zinc Sulfate |
| description |
The vertebrate olfactory system has fascinated neurobiologists over the last six decades because of its ability to replace its neurons and synaptic connections continuously throughout adult life, under both physiological and pathological conditions. Among the factors that are proposed to be involved in this regenerative potential, brain-derived neurotrophic factor (BDNF) is a candidate for having an important role in the neuronal turnover in the olfactory epithelium (OE) because of its well-documented neurogenic and trophic effects throughout the nervous system. The aim of the present study was to generate a suitable model to study the participation of BDNF in the recovery of the OE after injury in vivo. We developed an experimental design in which the OE of Rhinella arenarum tadpoles could be easily and selectively damaged by immersing the animals in ZnSO4 solutions of various concentrations for differing time periods. Image analysis of histological sections showed that different combinations of each of these conditions produced statistically different degrees of injury to the olfactory tissue. We also observed that the morphology of the OE was restored within a few days of recovery after ZnSO 4 treatment. Immunohistochemical analysis of BDNF was performed with an antiserum whose specificity was confirmed by Western blotting, and which showed drastic changes in the abundance and distribution pattern of this neurotrophin in the damaged olfactory system. Our results thus suggest that BDNF is involved in the regeneration of the OE of amphibian larvae, and that our approach is suitable for further investigations of this topic. © Springer-Verlag 2009. |
| author |
Yovanovich, Carola Anabel Mariana Jungblut, Lucas David Pozzi, Andrea Gabriela Paz, Dante Agustin |
| author_facet |
Yovanovich, Carola Anabel Mariana Jungblut, Lucas David Pozzi, Andrea Gabriela Paz, Dante Agustin |
| author_sort |
Yovanovich, Carola Anabel Mariana |
| title |
Amphibian larvae and zinc sulphate: A suitable model to study the role of brain-derived neurotrophic factor (BDNF) in the neuronal turnover of the olfactory epithelium |
| title_short |
Amphibian larvae and zinc sulphate: A suitable model to study the role of brain-derived neurotrophic factor (BDNF) in the neuronal turnover of the olfactory epithelium |
| title_full |
Amphibian larvae and zinc sulphate: A suitable model to study the role of brain-derived neurotrophic factor (BDNF) in the neuronal turnover of the olfactory epithelium |
| title_fullStr |
Amphibian larvae and zinc sulphate: A suitable model to study the role of brain-derived neurotrophic factor (BDNF) in the neuronal turnover of the olfactory epithelium |
| title_full_unstemmed |
Amphibian larvae and zinc sulphate: A suitable model to study the role of brain-derived neurotrophic factor (BDNF) in the neuronal turnover of the olfactory epithelium |
| title_sort |
amphibian larvae and zinc sulphate: a suitable model to study the role of brain-derived neurotrophic factor (bdnf) in the neuronal turnover of the olfactory epithelium |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0302766X_v336_n1_p1_Yovanovich http://hdl.handle.net/20.500.12110/paper_0302766X_v336_n1_p1_Yovanovich |
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