Extracellular biosynthesis of bactericidal Ag/AgCl nanoparticles for crop protection using the fungus Macrophomina phaseolina
Synthesis of noble metal nanoparticles using natural products and living organisms has drawn a lot of interest owing to economic prospects and potential applicability in different fields. For this work we used the exudate of the soil fungus Macrophomina phaseolina for a low-cost method of green synt...
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2019
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03014797_v231_n_p457_Spagnoletti http://hdl.handle.net/20.500.12110/paper_03014797_v231_n_p457_Spagnoletti |
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paper:paper_03014797_v231_n_p457_Spagnoletti2023-06-08T15:27:57Z Extracellular biosynthesis of bactericidal Ag/AgCl nanoparticles for crop protection using the fungus Macrophomina phaseolina Crop protection Glycine max Macrophomina phaseolina Nanotechnology Phytopathogen control Silver-silver chloride nanoparticles nanoparticle silver chloride silver nanoparticle antibiotics biological control biotechnology fungus nanoparticle nanotechnology oxidative stress pathogen silver soybean spectral analysis yeast antibacterial activity Article bacterial growth bactericidal activity biosynthesis cell membrane crop protection exudate germination Gram negative bacterium Gram positive bacterium green chemistry Macrophomina phaseolina membrane damage particle size process optimization scanning electron microscopy soybean surface plasmon resonance X ray diffraction zone of inhibition Fungi Glycine max Macrophomina phaseolina Negibacteria Posibacteria Synthesis of noble metal nanoparticles using natural products and living organisms has drawn a lot of interest owing to economic prospects and potential applicability in different fields. For this work we used the exudate of the soil fungus Macrophomina phaseolina for a low-cost method of green synthesis to obtain stable silver-silver chloride nanoparticles (Ag/AgCl-NPs). Reaction parameters including media and AgNO3 concentration were further optimized for NPs production. Spectral analysis revealed a peak at 420 nm that corresponds to the surface plasmon resonance of silver NPs. Scanning electron microscopy (SEM) analysis unveiled NPs spherical morphology with a size range of 5–30 nm. The crystalline nature of the synthesized NPs was examined by X-ray diffraction (XRD) analysis. The green synthesized NPs showed activity against gram-positive and gram-negative bacteria. No effect in fungi or yeast cells was detected, though a high inhibitory effect was observed on bacteria growth kinetics. Interaction of bacteria with Ag/AgCl-NPs led to cell membrane damage as observed by SEM, followed by an increase in oxidative stress, being this the possible mechanism behind the strong bactericidal activity depicted. In order to test its possible applicability as a seed protection agent the effect of Ag/AgCl-NPs dosage on soybean (Glycine max L.) seed's germination was also examined. Interestingly, not only the germination process was not affected by the NPs dosage or time of seeds incubation, but also no oxidative damage was detected in seeds after exposure to the biogenic nanoparticles. © 2018 Elsevier Ltd 2019 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03014797_v231_n_p457_Spagnoletti http://hdl.handle.net/20.500.12110/paper_03014797_v231_n_p457_Spagnoletti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Crop protection Glycine max Macrophomina phaseolina Nanotechnology Phytopathogen control Silver-silver chloride nanoparticles nanoparticle silver chloride silver nanoparticle antibiotics biological control biotechnology fungus nanoparticle nanotechnology oxidative stress pathogen silver soybean spectral analysis yeast antibacterial activity Article bacterial growth bactericidal activity biosynthesis cell membrane crop protection exudate germination Gram negative bacterium Gram positive bacterium green chemistry Macrophomina phaseolina membrane damage particle size process optimization scanning electron microscopy soybean surface plasmon resonance X ray diffraction zone of inhibition Fungi Glycine max Macrophomina phaseolina Negibacteria Posibacteria |
| spellingShingle |
Crop protection Glycine max Macrophomina phaseolina Nanotechnology Phytopathogen control Silver-silver chloride nanoparticles nanoparticle silver chloride silver nanoparticle antibiotics biological control biotechnology fungus nanoparticle nanotechnology oxidative stress pathogen silver soybean spectral analysis yeast antibacterial activity Article bacterial growth bactericidal activity biosynthesis cell membrane crop protection exudate germination Gram negative bacterium Gram positive bacterium green chemistry Macrophomina phaseolina membrane damage particle size process optimization scanning electron microscopy soybean surface plasmon resonance X ray diffraction zone of inhibition Fungi Glycine max Macrophomina phaseolina Negibacteria Posibacteria Extracellular biosynthesis of bactericidal Ag/AgCl nanoparticles for crop protection using the fungus Macrophomina phaseolina |
| topic_facet |
Crop protection Glycine max Macrophomina phaseolina Nanotechnology Phytopathogen control Silver-silver chloride nanoparticles nanoparticle silver chloride silver nanoparticle antibiotics biological control biotechnology fungus nanoparticle nanotechnology oxidative stress pathogen silver soybean spectral analysis yeast antibacterial activity Article bacterial growth bactericidal activity biosynthesis cell membrane crop protection exudate germination Gram negative bacterium Gram positive bacterium green chemistry Macrophomina phaseolina membrane damage particle size process optimization scanning electron microscopy soybean surface plasmon resonance X ray diffraction zone of inhibition Fungi Glycine max Macrophomina phaseolina Negibacteria Posibacteria |
| description |
Synthesis of noble metal nanoparticles using natural products and living organisms has drawn a lot of interest owing to economic prospects and potential applicability in different fields. For this work we used the exudate of the soil fungus Macrophomina phaseolina for a low-cost method of green synthesis to obtain stable silver-silver chloride nanoparticles (Ag/AgCl-NPs). Reaction parameters including media and AgNO3 concentration were further optimized for NPs production. Spectral analysis revealed a peak at 420 nm that corresponds to the surface plasmon resonance of silver NPs. Scanning electron microscopy (SEM) analysis unveiled NPs spherical morphology with a size range of 5–30 nm. The crystalline nature of the synthesized NPs was examined by X-ray diffraction (XRD) analysis. The green synthesized NPs showed activity against gram-positive and gram-negative bacteria. No effect in fungi or yeast cells was detected, though a high inhibitory effect was observed on bacteria growth kinetics. Interaction of bacteria with Ag/AgCl-NPs led to cell membrane damage as observed by SEM, followed by an increase in oxidative stress, being this the possible mechanism behind the strong bactericidal activity depicted. In order to test its possible applicability as a seed protection agent the effect of Ag/AgCl-NPs dosage on soybean (Glycine max L.) seed's germination was also examined. Interestingly, not only the germination process was not affected by the NPs dosage or time of seeds incubation, but also no oxidative damage was detected in seeds after exposure to the biogenic nanoparticles. © 2018 Elsevier Ltd |
| title |
Extracellular biosynthesis of bactericidal Ag/AgCl nanoparticles for crop protection using the fungus Macrophomina phaseolina |
| title_short |
Extracellular biosynthesis of bactericidal Ag/AgCl nanoparticles for crop protection using the fungus Macrophomina phaseolina |
| title_full |
Extracellular biosynthesis of bactericidal Ag/AgCl nanoparticles for crop protection using the fungus Macrophomina phaseolina |
| title_fullStr |
Extracellular biosynthesis of bactericidal Ag/AgCl nanoparticles for crop protection using the fungus Macrophomina phaseolina |
| title_full_unstemmed |
Extracellular biosynthesis of bactericidal Ag/AgCl nanoparticles for crop protection using the fungus Macrophomina phaseolina |
| title_sort |
extracellular biosynthesis of bactericidal ag/agcl nanoparticles for crop protection using the fungus macrophomina phaseolina |
| publishDate |
2019 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03014797_v231_n_p457_Spagnoletti http://hdl.handle.net/20.500.12110/paper_03014797_v231_n_p457_Spagnoletti |
| _version_ |
1768545508900470784 |