Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes

Background: it is well known that culture cells secrete to their medium different factors that can alter their own as well as other cells' metabolism and functions. In the present study we evaluated the effect of culture supernatants originated from two cancer cell lines, Calu-1 (lung epidermoi...

Descripción completa

Detalles Bibliográficos
Publicado:	1999
Materias:	Culture supernatants Lung cancer cell lines Monocytes HLA DR antigen article cell culture culture medium drug effect fluorescent antibody technique human monocyte phagocytosis secretion Culture Media, Conditioned Fluorescent Antibody Technique, Indirect HLA-DR Antigens Humans Phagocytosis Tumor Cells, Cultured
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03010546_v27_n4_p195_Novellino http://hdl.handle.net/20.500.12110/paper_03010546_v27_n4_p195_Novellino
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03010546_v27_n4_p195_Novellino
record_format	dspace
spelling	paper:paper_03010546_v27_n4_p195_Novellino2023-06-08T15:27:51Z Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes Culture supernatants Lung cancer cell lines Monocytes HLA DR antigen article cell culture culture medium drug effect fluorescent antibody technique human monocyte phagocytosis secretion Culture Media, Conditioned Fluorescent Antibody Technique, Indirect HLA-DR Antigens Humans Monocytes Phagocytosis Tumor Cells, Cultured Background: it is well known that culture cells secrete to their medium different factors that can alter their own as well as other cells' metabolism and functions. In the present study we evaluated the effect of culture supernatants originated from two cancer cell lines, Calu-1 (lung epidermoid carcinoma) and A-427 (lung adenocarcinoma) on peripheral blood monocytes (MO) from 20 healthy donors (HD). Methods and results: MO were incubated with supernatants or with medium only (controls) during 2 or 18 hours. There were determined the expression of HLA-DR antigen by indirect immunofluorescence technique, the formation of reactive oxygen intermediates (NBT reduction capacity) and the phagocytic capacity (Candida albicans-anti Candida albicans system). These determinations were made also in MO from 16 patients with advanced lung cancer (LCP). Percentages of expression (media ± SE) were: HLA-DR (+) MO: Calu-1 = 30 ± 2; A-427 = 31 ± 2; LCP = 52 ± 3 (p < 0.0001 vs HD: 77 ± 1%) NBT (+)MO: Calu-1 = 47 ± 1; A-427 = 49 ± 1; LCP = 56 ± 2 (p < 0.01 vs HD: 45 ± 2%), Phagocytic MO: Calu-1 = 38 ± 3; A-427 = 39 ± 2; LCP = 32 ± 3 (p < 0.0001 vs HD: 50 ± 1%). Conclusions: we conclude that this in vitro model reproduced characteristics found in MO from lung cancer patients, since culture supernatants induced in normal MO phenotypic and functional characteristics also found in MO from patients analyzed. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03010546_v27_n4_p195_Novellino http://hdl.handle.net/20.500.12110/paper_03010546_v27_n4_p195_Novellino
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Culture supernatants Lung cancer cell lines Monocytes HLA DR antigen article cell culture culture medium drug effect fluorescent antibody technique human monocyte phagocytosis secretion Culture Media, Conditioned Fluorescent Antibody Technique, Indirect HLA-DR Antigens Humans Monocytes Phagocytosis Tumor Cells, Cultured
spellingShingle	Culture supernatants Lung cancer cell lines Monocytes HLA DR antigen article cell culture culture medium drug effect fluorescent antibody technique human monocyte phagocytosis secretion Culture Media, Conditioned Fluorescent Antibody Technique, Indirect HLA-DR Antigens Humans Monocytes Phagocytosis Tumor Cells, Cultured Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes
topic_facet	Culture supernatants Lung cancer cell lines Monocytes HLA DR antigen article cell culture culture medium drug effect fluorescent antibody technique human monocyte phagocytosis secretion Culture Media, Conditioned Fluorescent Antibody Technique, Indirect HLA-DR Antigens Humans Monocytes Phagocytosis Tumor Cells, Cultured
description	Background: it is well known that culture cells secrete to their medium different factors that can alter their own as well as other cells' metabolism and functions. In the present study we evaluated the effect of culture supernatants originated from two cancer cell lines, Calu-1 (lung epidermoid carcinoma) and A-427 (lung adenocarcinoma) on peripheral blood monocytes (MO) from 20 healthy donors (HD). Methods and results: MO were incubated with supernatants or with medium only (controls) during 2 or 18 hours. There were determined the expression of HLA-DR antigen by indirect immunofluorescence technique, the formation of reactive oxygen intermediates (NBT reduction capacity) and the phagocytic capacity (Candida albicans-anti Candida albicans system). These determinations were made also in MO from 16 patients with advanced lung cancer (LCP). Percentages of expression (media ± SE) were: HLA-DR (+) MO: Calu-1 = 30 ± 2; A-427 = 31 ± 2; LCP = 52 ± 3 (p < 0.0001 vs HD: 77 ± 1%) NBT (+)MO: Calu-1 = 47 ± 1; A-427 = 49 ± 1; LCP = 56 ± 2 (p < 0.01 vs HD: 45 ± 2%), Phagocytic MO: Calu-1 = 38 ± 3; A-427 = 39 ± 2; LCP = 32 ± 3 (p < 0.0001 vs HD: 50 ± 1%). Conclusions: we conclude that this in vitro model reproduced characteristics found in MO from lung cancer patients, since culture supernatants induced in normal MO phenotypic and functional characteristics also found in MO from patients analyzed.
title	Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes
title_short	Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes
title_full	Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes
title_fullStr	Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes
title_full_unstemmed	Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes
title_sort	effect of culture supernatants from two cancer cell lines on healthy donors' monocytes
publishDate	1999
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03010546_v27_n4_p195_Novellino http://hdl.handle.net/20.500.12110/paper_03010546_v27_n4_p195_Novellino
_version_	1768543799430086656

Effect of culture supernatants from two cancer cell lines on healthy donors' monocytes

Ejemplares similares