Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes

Objective: To investigate the kinetics of insulin-like growth factor-1 receptor (IGF-1R) expression in PHA-stimulated T lymphocytes. Methods: IGF-1R protein and mRNA were detected by flow cytometry and RT-PCR respectively, between 0 and 48 h after cell activation. Results: Few minutes after T lympho...

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Publicado:	2003
Materias:	Down-regulation Insulin-like growth factor-1 receptor T lymphocyte Up-regulation messenger RNA phytohemagglutinin somatomedin C receptor article cell membrane cell surface cell transport flow cytometry human human cell kinetics lymphocyte proliferation priority journal protein expression receptor upregulation reverse transcription polymerase chain reaction T lymphocyte activation Down-Regulation Flow Cytometry Humans Kinetics Lymphocyte Activation Receptor, IGF Type 1 Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger T-Lymphocytes Up-Regulation
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03010163_v59_n6_p276_Segretin http://hdl.handle.net/20.500.12110/paper_03010163_v59_n6_p276_Segretin
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03010163_v59_n6_p276_Segretin
record_format	dspace
spelling	paper:paper_03010163_v59_n6_p276_Segretin2023-06-08T15:27:51Z Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes Down-regulation Insulin-like growth factor-1 receptor T lymphocyte Up-regulation messenger RNA phytohemagglutinin somatomedin C receptor article cell membrane cell surface cell transport flow cytometry human human cell kinetics lymphocyte proliferation priority journal protein expression receptor upregulation reverse transcription polymerase chain reaction T lymphocyte T lymphocyte activation Down-Regulation Flow Cytometry Humans Kinetics Lymphocyte Activation Receptor, IGF Type 1 Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger T-Lymphocytes Up-Regulation Objective: To investigate the kinetics of insulin-like growth factor-1 receptor (IGF-1R) expression in PHA-stimulated T lymphocytes. Methods: IGF-1R protein and mRNA were detected by flow cytometry and RT-PCR respectively, between 0 and 48 h after cell activation. Results: Few minutes after T lymphocytes were activated, internalization of the IGF-1R from the cell membrane was observed, achieving the lower level between 1 and 6 h and was accompanied by a reduction in its mRNA. This was followed by re-expression of IGF-1R on the cell surface and an increase in IGF-1R mRNA levels in the cytoplasm, reaching levels higher than those recorded initially after 48 h activation. Conclusion: This down- and up-regulation suggests that restoration of IGF-1R would be the result of receptor recycling and de novo synthesis and highlights its importance for T lymphocyte proliferation. Copyright © 2003 S. Karger AG, Basel. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03010163_v59_n6_p276_Segretin http://hdl.handle.net/20.500.12110/paper_03010163_v59_n6_p276_Segretin
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Down-regulation Insulin-like growth factor-1 receptor T lymphocyte Up-regulation messenger RNA phytohemagglutinin somatomedin C receptor article cell membrane cell surface cell transport flow cytometry human human cell kinetics lymphocyte proliferation priority journal protein expression receptor upregulation reverse transcription polymerase chain reaction T lymphocyte T lymphocyte activation Down-Regulation Flow Cytometry Humans Kinetics Lymphocyte Activation Receptor, IGF Type 1 Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger T-Lymphocytes Up-Regulation
spellingShingle	Down-regulation Insulin-like growth factor-1 receptor T lymphocyte Up-regulation messenger RNA phytohemagglutinin somatomedin C receptor article cell membrane cell surface cell transport flow cytometry human human cell kinetics lymphocyte proliferation priority journal protein expression receptor upregulation reverse transcription polymerase chain reaction T lymphocyte T lymphocyte activation Down-Regulation Flow Cytometry Humans Kinetics Lymphocyte Activation Receptor, IGF Type 1 Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger T-Lymphocytes Up-Regulation Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes
topic_facet	Down-regulation Insulin-like growth factor-1 receptor T lymphocyte Up-regulation messenger RNA phytohemagglutinin somatomedin C receptor article cell membrane cell surface cell transport flow cytometry human human cell kinetics lymphocyte proliferation priority journal protein expression receptor upregulation reverse transcription polymerase chain reaction T lymphocyte T lymphocyte activation Down-Regulation Flow Cytometry Humans Kinetics Lymphocyte Activation Receptor, IGF Type 1 Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger T-Lymphocytes Up-Regulation
description	Objective: To investigate the kinetics of insulin-like growth factor-1 receptor (IGF-1R) expression in PHA-stimulated T lymphocytes. Methods: IGF-1R protein and mRNA were detected by flow cytometry and RT-PCR respectively, between 0 and 48 h after cell activation. Results: Few minutes after T lymphocytes were activated, internalization of the IGF-1R from the cell membrane was observed, achieving the lower level between 1 and 6 h and was accompanied by a reduction in its mRNA. This was followed by re-expression of IGF-1R on the cell surface and an increase in IGF-1R mRNA levels in the cytoplasm, reaching levels higher than those recorded initially after 48 h activation. Conclusion: This down- and up-regulation suggests that restoration of IGF-1R would be the result of receptor recycling and de novo synthesis and highlights its importance for T lymphocyte proliferation. Copyright © 2003 S. Karger AG, Basel.
title	Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes
title_short	Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes
title_full	Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes
title_fullStr	Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes
title_full_unstemmed	Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes
title_sort	insulin-like growth factor-1 receptor regulation in activated human t lymphocytes
publishDate	2003
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03010163_v59_n6_p276_Segretin http://hdl.handle.net/20.500.12110/paper_03010163_v59_n6_p276_Segretin
_version_	1768542551579557888

Insulin-like growth factor-1 receptor regulation in activated human T lymphocytes

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