MCAM knockdown impairs PPARγ expression and 3T3-L1 fibroblasts differentiation to adipocytes

We investigated for the first time the expression of melanoma cell adhesion molecule (MCAM) and its involvement in the differentiation of 3T3-L1 fibroblasts to adipocytes. We found that MCAM mRNA increased subsequent to the activation of the master regulator of adipogenesis, PPARγ, and this increase...

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Publicado:	2018
Materias:	3T3-L1 fibroblasts Adipogenesis C/EBP beta MCAM/CD146 PPAR gamma adiponectin beta catenin CCAAT enhancer binding protein beta CD146 antigen messenger RNA mitogen activated protein kinase perilipin 1 peroxisome proliferator activated receptor gamma Mcam protein, mouse 3T3-L1 cell line adipocyte adipogenesis animal cell Article cell differentiation cell maturation controlled study down regulation enzyme activation gene expression gene knockdown immunofluorescence microscopy mouse mRNA expression level NIH 3T3 cell line nonhuman protein expression level protein function quantitative analysis real time polymerase chain reaction upregulation Western blotting animal biosynthesis cytology fibroblast gene expression regulation genetics metabolism 3T3-L1 Cells Adipocytes Animals CD146 Antigen Cell Differentiation Fibroblasts Gene Expression Regulation Gene Knockdown Techniques Mice
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v448_n1-2_p299_Gabrielli http://hdl.handle.net/20.500.12110/paper_03008177_v448_n1-2_p299_Gabrielli
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03008177_v448_n1-2_p299_Gabrielli
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spelling	paper:paper_03008177_v448_n1-2_p299_Gabrielli2023-06-08T15:27:29Z MCAM knockdown impairs PPARγ expression and 3T3-L1 fibroblasts differentiation to adipocytes 3T3-L1 fibroblasts Adipogenesis C/EBP beta MCAM/CD146 PPAR gamma adiponectin beta catenin CCAAT enhancer binding protein beta CD146 antigen messenger RNA mitogen activated protein kinase perilipin 1 peroxisome proliferator activated receptor gamma CD146 antigen Mcam protein, mouse peroxisome proliferator activated receptor gamma 3T3-L1 cell line adipocyte adipogenesis animal cell Article cell differentiation cell maturation controlled study down regulation enzyme activation gene expression gene knockdown immunofluorescence microscopy mouse mRNA expression level NIH 3T3 cell line nonhuman protein expression level protein function quantitative analysis real time polymerase chain reaction upregulation Western blotting 3T3-L1 cell line adipocyte adipogenesis animal biosynthesis cytology fibroblast gene expression regulation gene knockdown genetics metabolism 3T3-L1 Cells Adipocytes Adipogenesis Animals CD146 Antigen Cell Differentiation Fibroblasts Gene Expression Regulation Gene Knockdown Techniques Mice PPAR gamma We investigated for the first time the expression of melanoma cell adhesion molecule (MCAM) and its involvement in the differentiation of 3T3-L1 fibroblasts to adipocytes. We found that MCAM mRNA increased subsequent to the activation of the master regulator of adipogenesis, PPARγ, and this increase was maintained in the mature adipocytes. On the other hand, MCAM knockdown impaired differentiation and induction of PPARγ as well as expression of genes activated by PPARγ. However, events that precede and are necessary for early PPARγ activation, such as C/EBPβ induction, β-catenin downregulation, and ERK activation, were not affected in the MCAM knockdown cells. In keeping with this, the increase in PPARγ mRNA that precedes MCAM induction was not altered in the knockdown cells. In conclusion, our findings suggest that MCAM is a gene upregulated and involved in maintaining PPARγ induction in the late but not in the early stages of 3T3-L1 fibroblasts adipogenesis. © 2018, Springer Science+Business Media, LLC, part of Springer Nature. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v448_n1-2_p299_Gabrielli http://hdl.handle.net/20.500.12110/paper_03008177_v448_n1-2_p299_Gabrielli
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	3T3-L1 fibroblasts Adipogenesis C/EBP beta MCAM/CD146 PPAR gamma adiponectin beta catenin CCAAT enhancer binding protein beta CD146 antigen messenger RNA mitogen activated protein kinase perilipin 1 peroxisome proliferator activated receptor gamma CD146 antigen Mcam protein, mouse peroxisome proliferator activated receptor gamma 3T3-L1 cell line adipocyte adipogenesis animal cell Article cell differentiation cell maturation controlled study down regulation enzyme activation gene expression gene knockdown immunofluorescence microscopy mouse mRNA expression level NIH 3T3 cell line nonhuman protein expression level protein function quantitative analysis real time polymerase chain reaction upregulation Western blotting 3T3-L1 cell line adipocyte adipogenesis animal biosynthesis cytology fibroblast gene expression regulation gene knockdown genetics metabolism 3T3-L1 Cells Adipocytes Adipogenesis Animals CD146 Antigen Cell Differentiation Fibroblasts Gene Expression Regulation Gene Knockdown Techniques Mice PPAR gamma
spellingShingle	3T3-L1 fibroblasts Adipogenesis C/EBP beta MCAM/CD146 PPAR gamma adiponectin beta catenin CCAAT enhancer binding protein beta CD146 antigen messenger RNA mitogen activated protein kinase perilipin 1 peroxisome proliferator activated receptor gamma CD146 antigen Mcam protein, mouse peroxisome proliferator activated receptor gamma 3T3-L1 cell line adipocyte adipogenesis animal cell Article cell differentiation cell maturation controlled study down regulation enzyme activation gene expression gene knockdown immunofluorescence microscopy mouse mRNA expression level NIH 3T3 cell line nonhuman protein expression level protein function quantitative analysis real time polymerase chain reaction upregulation Western blotting 3T3-L1 cell line adipocyte adipogenesis animal biosynthesis cytology fibroblast gene expression regulation gene knockdown genetics metabolism 3T3-L1 Cells Adipocytes Adipogenesis Animals CD146 Antigen Cell Differentiation Fibroblasts Gene Expression Regulation Gene Knockdown Techniques Mice PPAR gamma MCAM knockdown impairs PPARγ expression and 3T3-L1 fibroblasts differentiation to adipocytes
topic_facet	3T3-L1 fibroblasts Adipogenesis C/EBP beta MCAM/CD146 PPAR gamma adiponectin beta catenin CCAAT enhancer binding protein beta CD146 antigen messenger RNA mitogen activated protein kinase perilipin 1 peroxisome proliferator activated receptor gamma CD146 antigen Mcam protein, mouse peroxisome proliferator activated receptor gamma 3T3-L1 cell line adipocyte adipogenesis animal cell Article cell differentiation cell maturation controlled study down regulation enzyme activation gene expression gene knockdown immunofluorescence microscopy mouse mRNA expression level NIH 3T3 cell line nonhuman protein expression level protein function quantitative analysis real time polymerase chain reaction upregulation Western blotting 3T3-L1 cell line adipocyte adipogenesis animal biosynthesis cytology fibroblast gene expression regulation gene knockdown genetics metabolism 3T3-L1 Cells Adipocytes Adipogenesis Animals CD146 Antigen Cell Differentiation Fibroblasts Gene Expression Regulation Gene Knockdown Techniques Mice PPAR gamma
description	We investigated for the first time the expression of melanoma cell adhesion molecule (MCAM) and its involvement in the differentiation of 3T3-L1 fibroblasts to adipocytes. We found that MCAM mRNA increased subsequent to the activation of the master regulator of adipogenesis, PPARγ, and this increase was maintained in the mature adipocytes. On the other hand, MCAM knockdown impaired differentiation and induction of PPARγ as well as expression of genes activated by PPARγ. However, events that precede and are necessary for early PPARγ activation, such as C/EBPβ induction, β-catenin downregulation, and ERK activation, were not affected in the MCAM knockdown cells. In keeping with this, the increase in PPARγ mRNA that precedes MCAM induction was not altered in the knockdown cells. In conclusion, our findings suggest that MCAM is a gene upregulated and involved in maintaining PPARγ induction in the late but not in the early stages of 3T3-L1 fibroblasts adipogenesis. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
title	MCAM knockdown impairs PPARγ expression and 3T3-L1 fibroblasts differentiation to adipocytes
title_short	MCAM knockdown impairs PPARγ expression and 3T3-L1 fibroblasts differentiation to adipocytes
title_full	MCAM knockdown impairs PPARγ expression and 3T3-L1 fibroblasts differentiation to adipocytes
title_fullStr	MCAM knockdown impairs PPARγ expression and 3T3-L1 fibroblasts differentiation to adipocytes
title_full_unstemmed	MCAM knockdown impairs PPARγ expression and 3T3-L1 fibroblasts differentiation to adipocytes
title_sort	mcam knockdown impairs pparγ expression and 3t3-l1 fibroblasts differentiation to adipocytes
publishDate	2018
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v448_n1-2_p299_Gabrielli http://hdl.handle.net/20.500.12110/paper_03008177_v448_n1-2_p299_Gabrielli
_version_	1768541937306959872

MCAM knockdown impairs PPARγ expression and 3T3-L1 fibroblasts differentiation to adipocytes

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