Lead enhancement of 3T3-L1 fibroblasts differentiation to adipocytes involves ERK, C/EBPβ and PPARγ activation

Lead (Pb) is an environmental and industrial contaminant that still represents a public health problem. Elevated Pb exposure has been inversely correlated with femoral bone density and associated with osteoporosis. In the last years, it has been shown that inhibition of osteogenesis from mesenchymal...

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Autores principales:	Martini, Claudia Noemí, Gabrielli, Matías, Vila, María del Carmen
Publicado:	2018
Materias:	3T3-L1 fibroblasts Adipogenesis ERK Lead PPARγ CCAAT enhancer binding protein beta lead mitogen activated protein kinase peroxisome proliferator activated receptor gamma Cebpb protein, mouse 3T3-L1 cell line adipocyte adipogenesis animal cell Article cell differentiation enzyme activation gene expression mitosis nonhuman phenotype animal drug effect fibroblast MAPK signaling metabolism mouse pathology 3T3-L1 Cells Adipocytes Animals CCAAT-Enhancer-Binding Protein-beta Fibroblasts MAP Kinase Signaling System Mice PPAR gamma
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v437_n1-2_p37_Martini http://hdl.handle.net/20.500.12110/paper_03008177_v437_n1-2_p37_Martini
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03008177_v437_n1-2_p37_Martini
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spelling	paper:paper_03008177_v437_n1-2_p37_Martini2023-06-08T15:27:28Z Lead enhancement of 3T3-L1 fibroblasts differentiation to adipocytes involves ERK, C/EBPβ and PPARγ activation Martini, Claudia Noemí Gabrielli, Matías Vila, María del Carmen 3T3-L1 fibroblasts Adipogenesis ERK Lead PPARγ CCAAT enhancer binding protein beta lead mitogen activated protein kinase peroxisome proliferator activated receptor gamma CCAAT enhancer binding protein beta Cebpb protein, mouse lead peroxisome proliferator activated receptor gamma 3T3-L1 cell line adipocyte adipogenesis animal cell Article cell differentiation enzyme activation gene expression mitosis nonhuman phenotype adipocyte animal drug effect fibroblast MAPK signaling metabolism mouse pathology 3T3-L1 Cells Adipocytes Adipogenesis Animals CCAAT-Enhancer-Binding Protein-beta Fibroblasts Lead MAP Kinase Signaling System Mice PPAR gamma Lead (Pb) is an environmental and industrial contaminant that still represents a public health problem. Elevated Pb exposure has been inversely correlated with femoral bone density and associated with osteoporosis. In the last years, it has been shown that inhibition of osteogenesis from mesenchymal stem cells activates adipogenesis and vice versa. In this paper, we investigated the effect of Pb on the differentiation of 3T3-L1 fibroblasts to adipocytes which is the cell model most used to study adipogenesis. After induction of differentiation, 2 days post-confluent cells re-enter the cell cycle and undergo mitotic clonal expansion (MCE) followed by expression of genes that produce the adipocyte phenotype. The presence of concentrations of Pb up to 10 μM during differentiation of 3T3-L1 fibroblasts did not interfere with MCE but enhanced the accumulation of cytosolic lipids that occur during adipogenesis, as well as, the induction of PPARγ, the master gene in adipogenesis. It is known that PPARγ upregulation is subsequent to induction of C/EBPβ and ERK activation, which are early events in adipogenesis. We found that both events were enhanced by Pb treatment. Our results support a stimulatory effect of Pb on adipogenesis which involves ERK activation and C/EBPβ upregulation prior to PPARγ and adipogenesis activation. © 2017, Springer Science+Business Media, LLC. Fil:Martini, C.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gabrielli, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vila, M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v437_n1-2_p37_Martini http://hdl.handle.net/20.500.12110/paper_03008177_v437_n1-2_p37_Martini
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	3T3-L1 fibroblasts Adipogenesis ERK Lead PPARγ CCAAT enhancer binding protein beta lead mitogen activated protein kinase peroxisome proliferator activated receptor gamma CCAAT enhancer binding protein beta Cebpb protein, mouse lead peroxisome proliferator activated receptor gamma 3T3-L1 cell line adipocyte adipogenesis animal cell Article cell differentiation enzyme activation gene expression mitosis nonhuman phenotype adipocyte animal drug effect fibroblast MAPK signaling metabolism mouse pathology 3T3-L1 Cells Adipocytes Adipogenesis Animals CCAAT-Enhancer-Binding Protein-beta Fibroblasts Lead MAP Kinase Signaling System Mice PPAR gamma
spellingShingle	3T3-L1 fibroblasts Adipogenesis ERK Lead PPARγ CCAAT enhancer binding protein beta lead mitogen activated protein kinase peroxisome proliferator activated receptor gamma CCAAT enhancer binding protein beta Cebpb protein, mouse lead peroxisome proliferator activated receptor gamma 3T3-L1 cell line adipocyte adipogenesis animal cell Article cell differentiation enzyme activation gene expression mitosis nonhuman phenotype adipocyte animal drug effect fibroblast MAPK signaling metabolism mouse pathology 3T3-L1 Cells Adipocytes Adipogenesis Animals CCAAT-Enhancer-Binding Protein-beta Fibroblasts Lead MAP Kinase Signaling System Mice PPAR gamma Martini, Claudia Noemí Gabrielli, Matías Vila, María del Carmen Lead enhancement of 3T3-L1 fibroblasts differentiation to adipocytes involves ERK, C/EBPβ and PPARγ activation
topic_facet	3T3-L1 fibroblasts Adipogenesis ERK Lead PPARγ CCAAT enhancer binding protein beta lead mitogen activated protein kinase peroxisome proliferator activated receptor gamma CCAAT enhancer binding protein beta Cebpb protein, mouse lead peroxisome proliferator activated receptor gamma 3T3-L1 cell line adipocyte adipogenesis animal cell Article cell differentiation enzyme activation gene expression mitosis nonhuman phenotype adipocyte animal drug effect fibroblast MAPK signaling metabolism mouse pathology 3T3-L1 Cells Adipocytes Adipogenesis Animals CCAAT-Enhancer-Binding Protein-beta Fibroblasts Lead MAP Kinase Signaling System Mice PPAR gamma
description	Lead (Pb) is an environmental and industrial contaminant that still represents a public health problem. Elevated Pb exposure has been inversely correlated with femoral bone density and associated with osteoporosis. In the last years, it has been shown that inhibition of osteogenesis from mesenchymal stem cells activates adipogenesis and vice versa. In this paper, we investigated the effect of Pb on the differentiation of 3T3-L1 fibroblasts to adipocytes which is the cell model most used to study adipogenesis. After induction of differentiation, 2 days post-confluent cells re-enter the cell cycle and undergo mitotic clonal expansion (MCE) followed by expression of genes that produce the adipocyte phenotype. The presence of concentrations of Pb up to 10 μM during differentiation of 3T3-L1 fibroblasts did not interfere with MCE but enhanced the accumulation of cytosolic lipids that occur during adipogenesis, as well as, the induction of PPARγ, the master gene in adipogenesis. It is known that PPARγ upregulation is subsequent to induction of C/EBPβ and ERK activation, which are early events in adipogenesis. We found that both events were enhanced by Pb treatment. Our results support a stimulatory effect of Pb on adipogenesis which involves ERK activation and C/EBPβ upregulation prior to PPARγ and adipogenesis activation. © 2017, Springer Science+Business Media, LLC.
author	Martini, Claudia Noemí Gabrielli, Matías Vila, María del Carmen
author_facet	Martini, Claudia Noemí Gabrielli, Matías Vila, María del Carmen
author_sort	Martini, Claudia Noemí
title	Lead enhancement of 3T3-L1 fibroblasts differentiation to adipocytes involves ERK, C/EBPβ and PPARγ activation
title_short	Lead enhancement of 3T3-L1 fibroblasts differentiation to adipocytes involves ERK, C/EBPβ and PPARγ activation
title_full	Lead enhancement of 3T3-L1 fibroblasts differentiation to adipocytes involves ERK, C/EBPβ and PPARγ activation
title_fullStr	Lead enhancement of 3T3-L1 fibroblasts differentiation to adipocytes involves ERK, C/EBPβ and PPARγ activation
title_full_unstemmed	Lead enhancement of 3T3-L1 fibroblasts differentiation to adipocytes involves ERK, C/EBPβ and PPARγ activation
title_sort	lead enhancement of 3t3-l1 fibroblasts differentiation to adipocytes involves erk, c/ebpβ and pparγ activation
publishDate	2018
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v437_n1-2_p37_Martini http://hdl.handle.net/20.500.12110/paper_03008177_v437_n1-2_p37_Martini
work_keys_str_mv	AT martiniclaudianoemi leadenhancementof3t3l1fibroblastsdifferentiationtoadipocytesinvolveserkcebpbandppargactivation AT gabriellimatias leadenhancementof3t3l1fibroblastsdifferentiationtoadipocytesinvolveserkcebpbandppargactivation AT vilamariadelcarmen leadenhancementof3t3l1fibroblastsdifferentiationtoadipocytesinvolveserkcebpbandppargactivation
_version_	1768546208432783360

Lead enhancement of 3T3-L1 fibroblasts differentiation to adipocytes involves ERK, C/EBPβ and PPARγ activation

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