Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus

A galactofuranosyl-containing glycopeptide has been isolated from mycelium of Ascobolus furfuraceus by extraction with water. The glycoconjugate was purified by DEAE-cellulose chromatography followed by gel filtration. A molecular weight of about 20 000 was determined by the latter method using stan...

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Autores principales:	Groisman, José Felipe, Mercuri, Oscar Alberto, Muchnik de Lederkremer, Rosa María
Publicado:	1986
Materias:	Ascobolus furfuraceus galactofuranosyl-containing glycopeptide glycopeptide beta galactofuranose fungus nonhuman
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02820080_v3_n1_p45_Groisman http://hdl.handle.net/20.500.12110/paper_02820080_v3_n1_p45_Groisman
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_02820080_v3_n1_p45_Groisman
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spelling	paper:paper_02820080_v3_n1_p45_Groisman2023-06-08T15:27:00Z Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus Groisman, José Felipe Mercuri, Oscar Alberto Muchnik de Lederkremer, Rosa María Ascobolus furfuraceus galactofuranosyl-containing glycopeptide glycopeptide beta galactofuranose fungus nonhuman A galactofuranosyl-containing glycopeptide has been isolated from mycelium of Ascobolus furfuraceus by extraction with water. The glycoconjugate was purified by DEAE-cellulose chromatography followed by gel filtration. A molecular weight of about 20 000 was determined by the latter method using standard dextrans. Neutral sugars accounted for 94.5% of the glycopeptide and were characterized as mannose, galactose, and glucose. Glucosamine was estimated colorimetrically (1.8%). The molar ratio of Man:Gal:Glc:GlcNH2 was 68:32:16:2. A trace amount of total phosphorus (0.2%) was found. The predominant amino acids were threonine and serine. The peptide moiety was labeled with [14C]formaldehyde and the elution of radioactivity was coincident with sugar on gel filtration in the presence of sodium dodecyl sulfate. The peak of radioactivity was retarded on release of galactose by mild acid hydrolysis. These results confirm the sugar-peptide linkage. © 1986 Glycoconjugate Journal. Fil:Groisman, J.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mercuri, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1986 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02820080_v3_n1_p45_Groisman http://hdl.handle.net/20.500.12110/paper_02820080_v3_n1_p45_Groisman
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Ascobolus furfuraceus galactofuranosyl-containing glycopeptide glycopeptide beta galactofuranose fungus nonhuman
spellingShingle	Ascobolus furfuraceus galactofuranosyl-containing glycopeptide glycopeptide beta galactofuranose fungus nonhuman Groisman, José Felipe Mercuri, Oscar Alberto Muchnik de Lederkremer, Rosa María Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus
topic_facet	Ascobolus furfuraceus galactofuranosyl-containing glycopeptide glycopeptide beta galactofuranose fungus nonhuman
description	A galactofuranosyl-containing glycopeptide has been isolated from mycelium of Ascobolus furfuraceus by extraction with water. The glycoconjugate was purified by DEAE-cellulose chromatography followed by gel filtration. A molecular weight of about 20 000 was determined by the latter method using standard dextrans. Neutral sugars accounted for 94.5% of the glycopeptide and were characterized as mannose, galactose, and glucose. Glucosamine was estimated colorimetrically (1.8%). The molar ratio of Man:Gal:Glc:GlcNH2 was 68:32:16:2. A trace amount of total phosphorus (0.2%) was found. The predominant amino acids were threonine and serine. The peptide moiety was labeled with [14C]formaldehyde and the elution of radioactivity was coincident with sugar on gel filtration in the presence of sodium dodecyl sulfate. The peak of radioactivity was retarded on release of galactose by mild acid hydrolysis. These results confirm the sugar-peptide linkage. © 1986 Glycoconjugate Journal.
author	Groisman, José Felipe Mercuri, Oscar Alberto Muchnik de Lederkremer, Rosa María
author_facet	Groisman, José Felipe Mercuri, Oscar Alberto Muchnik de Lederkremer, Rosa María
author_sort	Groisman, José Felipe
title	Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus
title_short	Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus
title_full	Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus
title_fullStr	Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus
title_full_unstemmed	Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus
title_sort	isolation and structural studies on a galactofuranosyl-containing glycopeptide from ascobolus furfuraceus
publishDate	1986
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02820080_v3_n1_p45_Groisman http://hdl.handle.net/20.500.12110/paper_02820080_v3_n1_p45_Groisman
work_keys_str_mv	AT groismanjosefelipe isolationandstructuralstudiesonagalactofuranosylcontainingglycopeptidefromascobolusfurfuraceus AT mercurioscaralberto isolationandstructuralstudiesonagalactofuranosylcontainingglycopeptidefromascobolusfurfuraceus AT muchnikdelederkremerrosamaria isolationandstructuralstudiesonagalactofuranosylcontainingglycopeptidefromascobolusfurfuraceus
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Isolation and structural studies on a galactofuranosyl-containing glycopeptide from Ascobolus furfuraceus

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