Acid Pullulanase from Bacillus polymyxa MIR-23

Within the frame of a screening program aimed at the isolation of amylolytic sporeformers, one strain with high amylolytic activity designated MIR-23 was selected. The microbial characterization was carried out by morphological and biochemical tests and, by means of statistical treatment, was identi...

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Detalles Bibliográficos
Autores principales: Castro, Guillermo Raúl, Siñeriz, Faustino
Publicado: 1992
Materias:
α-glucosidase
acidophilic microorganism
Bacillus polymyxa
batch culture
pullulanase, α-amylase
starch degradation
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02732289_v37_n3_p227_Castro
http://hdl.handle.net/20.500.12110/paper_02732289_v37_n3_p227_Castro
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_02732289_v37_n3_p227_Castro
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spelling paper:paper_02732289_v37_n3_p227_Castro2023-06-08T15:25:47Z Acid Pullulanase from Bacillus polymyxa MIR-23 Castro, Guillermo Raúl Siñeriz, Faustino α-glucosidase acidophilic microorganism Bacillus polymyxa batch culture pullulanase, α-amylase starch degradation Within the frame of a screening program aimed at the isolation of amylolytic sporeformers, one strain with high amylolytic activity designated MIR-23 was selected. The microbial characterization was carried out by morphological and biochemical tests and, by means of statistical treatment, was identified as Bacillus polymyxa. The organism could grow in acidic conditions (pH 5.0) on a starch medium and produce α-amylase, pullulanase, and α-glucosidase. Batch cultures showed the highest enzyme activities in the stationary phase. Pullulanase activity exhibited an optimal temperature of 52-57°C at pH 4.5-5.5. These properties would allow its use in the saccharification processes in the starch industries. © 1993 The Humana Press Inc. Fil:Castro, G.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Siñeriz, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1992 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02732289_v37_n3_p227_Castro http://hdl.handle.net/20.500.12110/paper_02732289_v37_n3_p227_Castro
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic α-glucosidase
acidophilic microorganism
Bacillus polymyxa
batch culture
pullulanase, α-amylase
starch degradation
spellingShingle α-glucosidase
acidophilic microorganism
Bacillus polymyxa
batch culture
pullulanase, α-amylase
starch degradation
Castro, Guillermo Raúl
Siñeriz, Faustino
Acid Pullulanase from Bacillus polymyxa MIR-23
topic_facet α-glucosidase
acidophilic microorganism
Bacillus polymyxa
batch culture
pullulanase, α-amylase
starch degradation
description Within the frame of a screening program aimed at the isolation of amylolytic sporeformers, one strain with high amylolytic activity designated MIR-23 was selected. The microbial characterization was carried out by morphological and biochemical tests and, by means of statistical treatment, was identified as Bacillus polymyxa. The organism could grow in acidic conditions (pH 5.0) on a starch medium and produce α-amylase, pullulanase, and α-glucosidase. Batch cultures showed the highest enzyme activities in the stationary phase. Pullulanase activity exhibited an optimal temperature of 52-57°C at pH 4.5-5.5. These properties would allow its use in the saccharification processes in the starch industries. © 1993 The Humana Press Inc.
author Castro, Guillermo Raúl
Siñeriz, Faustino
author_facet Castro, Guillermo Raúl
Siñeriz, Faustino
author_sort Castro, Guillermo Raúl
title Acid Pullulanase from Bacillus polymyxa MIR-23
title_short Acid Pullulanase from Bacillus polymyxa MIR-23
title_full Acid Pullulanase from Bacillus polymyxa MIR-23
title_fullStr Acid Pullulanase from Bacillus polymyxa MIR-23
title_full_unstemmed Acid Pullulanase from Bacillus polymyxa MIR-23
title_sort acid pullulanase from bacillus polymyxa mir-23
publishDate 1992
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02732289_v37_n3_p227_Castro
http://hdl.handle.net/20.500.12110/paper_02732289_v37_n3_p227_Castro
work_keys_str_mv AT castroguillermoraul acidpullulanasefrombacilluspolymyxamir23
AT sinerizfaustino acidpullulanasefrombacilluspolymyxamir23
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