The Antigenic Mucin‐Like Glycoproteins of Human Seminal Plasma

ABSTRACT: Immunodiffusion, immunoelectrophoresis, SDS‐polyacrylamide gel electrophoresis, polyacrylamide gel electrophoresis, ultracentrifugation analysis, and gel permeation chromatography in chaotropic and detergent mediums showed that the human seminal plasma obtained and stored in the usual way...

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Autores principales:	Tortorella, Hélen, Mazzini, María Nélida, Cerezo, Alberto Saúl
Publicado:	1983
Materias:	glycoprotein mucin gel chromatography human human cell immunodiffusion immunoelectrophoresis male genital system polyacrylamide gel electrophoresis sperm Chemistry Comparative Study Glycoproteins Human Male Molecular Weight Mucins Semen Support, Non-U.S. Gov't
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02717352_v4_n2_p76_TORTORELLA http://hdl.handle.net/20.500.12110/paper_02717352_v4_n2_p76_TORTORELLA
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_02717352_v4_n2_p76_TORTORELLA
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spelling	paper:paper_02717352_v4_n2_p76_TORTORELLA2023-06-08T15:24:59Z The Antigenic Mucin‐Like Glycoproteins of Human Seminal Plasma Tortorella, Hélen Mazzini, María Nélida Cerezo, Alberto Saúl glycoprotein mucin gel chromatography human human cell immunodiffusion immunoelectrophoresis male genital system polyacrylamide gel electrophoresis sperm Chemistry Comparative Study Glycoproteins Human Male Molecular Weight Mucins Semen Support, Non-U.S. Gov't ABSTRACT: Immunodiffusion, immunoelectrophoresis, SDS‐polyacrylamide gel electrophoresis, polyacrylamide gel electrophoresis, ultracentrifugation analysis, and gel permeation chromatography in chaotropic and detergent mediums showed that the human seminal plasma obtained and stored in the usual way is mainly composed by heterodispersed glycoproteins of low molecular weight. The glycoprotein components of the human seminal plasma which do not interact specifically with concanavalin A (con A) were separated by column chromatography on Con A‐Sepharose according to nonspecificion exchange and hydrophobic interactions with the protein. The carbohydrate composition of the glycoproteins and the existence of the N‐acetylgalactosaminylserine (or threonine) linkage, as well as their aggregation properties show that they are mucin‐type glycoproteins. They resulted “immunologically pure’ although the carbohydrate chains show the structural heterodispersion usually found in mucins. The glycoproteins have a common structural pattern for the terminal nonreducing, determinant‐bearing sequences oftheir oligosaccharide chains which, together with the low molecular weights and the known fact that mucus secretions of normal cells contain only one or two types of glycoproteins, suggest that they are fragments produced by endogenous proteolytic degradation of a native mucin. 1983 Munksgaard Fil:TORTORELLA, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:MAZZINI, M.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:CEREZO, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1983 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02717352_v4_n2_p76_TORTORELLA http://hdl.handle.net/20.500.12110/paper_02717352_v4_n2_p76_TORTORELLA
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	glycoprotein mucin gel chromatography human human cell immunodiffusion immunoelectrophoresis male genital system polyacrylamide gel electrophoresis sperm Chemistry Comparative Study Glycoproteins Human Male Molecular Weight Mucins Semen Support, Non-U.S. Gov't
spellingShingle	glycoprotein mucin gel chromatography human human cell immunodiffusion immunoelectrophoresis male genital system polyacrylamide gel electrophoresis sperm Chemistry Comparative Study Glycoproteins Human Male Molecular Weight Mucins Semen Support, Non-U.S. Gov't Tortorella, Hélen Mazzini, María Nélida Cerezo, Alberto Saúl The Antigenic Mucin‐Like Glycoproteins of Human Seminal Plasma
topic_facet	glycoprotein mucin gel chromatography human human cell immunodiffusion immunoelectrophoresis male genital system polyacrylamide gel electrophoresis sperm Chemistry Comparative Study Glycoproteins Human Male Molecular Weight Mucins Semen Support, Non-U.S. Gov't
description	ABSTRACT: Immunodiffusion, immunoelectrophoresis, SDS‐polyacrylamide gel electrophoresis, polyacrylamide gel electrophoresis, ultracentrifugation analysis, and gel permeation chromatography in chaotropic and detergent mediums showed that the human seminal plasma obtained and stored in the usual way is mainly composed by heterodispersed glycoproteins of low molecular weight. The glycoprotein components of the human seminal plasma which do not interact specifically with concanavalin A (con A) were separated by column chromatography on Con A‐Sepharose according to nonspecificion exchange and hydrophobic interactions with the protein. The carbohydrate composition of the glycoproteins and the existence of the N‐acetylgalactosaminylserine (or threonine) linkage, as well as their aggregation properties show that they are mucin‐type glycoproteins. They resulted “immunologically pure’ although the carbohydrate chains show the structural heterodispersion usually found in mucins. The glycoproteins have a common structural pattern for the terminal nonreducing, determinant‐bearing sequences oftheir oligosaccharide chains which, together with the low molecular weights and the known fact that mucus secretions of normal cells contain only one or two types of glycoproteins, suggest that they are fragments produced by endogenous proteolytic degradation of a native mucin. 1983 Munksgaard
author	Tortorella, Hélen Mazzini, María Nélida Cerezo, Alberto Saúl
author_facet	Tortorella, Hélen Mazzini, María Nélida Cerezo, Alberto Saúl
author_sort	Tortorella, Hélen
title	The Antigenic Mucin‐Like Glycoproteins of Human Seminal Plasma
title_short	The Antigenic Mucin‐Like Glycoproteins of Human Seminal Plasma
title_full	The Antigenic Mucin‐Like Glycoproteins of Human Seminal Plasma
title_fullStr	The Antigenic Mucin‐Like Glycoproteins of Human Seminal Plasma
title_full_unstemmed	The Antigenic Mucin‐Like Glycoproteins of Human Seminal Plasma
title_sort	antigenic mucin‐like glycoproteins of human seminal plasma
publishDate	1983
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02717352_v4_n2_p76_TORTORELLA http://hdl.handle.net/20.500.12110/paper_02717352_v4_n2_p76_TORTORELLA
work_keys_str_mv	AT tortorellahelen theantigenicmucinlikeglycoproteinsofhumanseminalplasma AT mazzinimarianelida theantigenicmucinlikeglycoproteinsofhumanseminalplasma AT cerezoalbertosaul theantigenicmucinlikeglycoproteinsofhumanseminalplasma AT tortorellahelen antigenicmucinlikeglycoproteinsofhumanseminalplasma AT mazzinimarianelida antigenicmucinlikeglycoproteinsofhumanseminalplasma AT cerezoalbertosaul antigenicmucinlikeglycoproteinsofhumanseminalplasma
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The Antigenic Mucin‐Like Glycoproteins of Human Seminal Plasma

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