Photodynamic therapy of activated and resting lymphocytes and its antioxidant adaptive response

In this work we have studied the effects of ALA-mediated photodynamic therapy (PDT) on resting and mitogen-activated murine splenic lymphocytes, evaluating its impact on cell viability. We have also characterised the stress response, measuring the levels of antioxidant enzymes. A 2 h exposure to ALA...

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Publicado:	2002
Materias:	5-Aminolaevulinic acid Catalase Glutathione peroxidase Lymphocytes PDT Photodynamic therapy Superoxide dismutase
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02688921_v17_n1_p42_Casas http://hdl.handle.net/20.500.12110/paper_02688921_v17_n1_p42_Casas
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_02688921_v17_n1_p42_Casas
record_format	dspace
spelling	paper:paper_02688921_v17_n1_p42_Casas2023-06-08T15:24:13Z Photodynamic therapy of activated and resting lymphocytes and its antioxidant adaptive response 5-Aminolaevulinic acid Catalase Glutathione peroxidase Lymphocytes PDT Photodynamic therapy Superoxide dismutase In this work we have studied the effects of ALA-mediated photodynamic therapy (PDT) on resting and mitogen-activated murine splenic lymphocytes, evaluating its impact on cell viability. We have also characterised the stress response, measuring the levels of antioxidant enzymes. A 2 h exposure to ALA produced 50% lethality upon irradiation of activated cells with 2.1 J/cm2. The decrease in cell survival with increasing time exposure to ALA, correlated well with the higher porphyrin accumulation. In resting lymphocytes, in spite of the low amount of porphyrins formed during 2 h incubation with ALA, 40% of the cells died after irradiation, this response was not further increased when higher amounts of porphyrins were synthesised. Superoxide dismutase was impaired by light treatment independently of ALA exposure in activated lymphocytes and, to a lesser extent, in resting lymphocytes. PDT induced an antioxidant adaptive response in activated cells 19 h after irradiation, reflected as a net increase in GSHPx activity and a slight reversion of the catalase (CAT) activity already impaired by light treatment. PDT treatment of activated cells also produced a diminution in the GSH/GSSG ratio. Only activated cells are capable of developing an antioxidant adaptive response to PDT treatment. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02688921_v17_n1_p42_Casas http://hdl.handle.net/20.500.12110/paper_02688921_v17_n1_p42_Casas
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	5-Aminolaevulinic acid Catalase Glutathione peroxidase Lymphocytes PDT Photodynamic therapy Superoxide dismutase
spellingShingle	5-Aminolaevulinic acid Catalase Glutathione peroxidase Lymphocytes PDT Photodynamic therapy Superoxide dismutase Photodynamic therapy of activated and resting lymphocytes and its antioxidant adaptive response
topic_facet	5-Aminolaevulinic acid Catalase Glutathione peroxidase Lymphocytes PDT Photodynamic therapy Superoxide dismutase
description	In this work we have studied the effects of ALA-mediated photodynamic therapy (PDT) on resting and mitogen-activated murine splenic lymphocytes, evaluating its impact on cell viability. We have also characterised the stress response, measuring the levels of antioxidant enzymes. A 2 h exposure to ALA produced 50% lethality upon irradiation of activated cells with 2.1 J/cm2. The decrease in cell survival with increasing time exposure to ALA, correlated well with the higher porphyrin accumulation. In resting lymphocytes, in spite of the low amount of porphyrins formed during 2 h incubation with ALA, 40% of the cells died after irradiation, this response was not further increased when higher amounts of porphyrins were synthesised. Superoxide dismutase was impaired by light treatment independently of ALA exposure in activated lymphocytes and, to a lesser extent, in resting lymphocytes. PDT induced an antioxidant adaptive response in activated cells 19 h after irradiation, reflected as a net increase in GSHPx activity and a slight reversion of the catalase (CAT) activity already impaired by light treatment. PDT treatment of activated cells also produced a diminution in the GSH/GSSG ratio. Only activated cells are capable of developing an antioxidant adaptive response to PDT treatment.
title	Photodynamic therapy of activated and resting lymphocytes and its antioxidant adaptive response
title_short	Photodynamic therapy of activated and resting lymphocytes and its antioxidant adaptive response
title_full	Photodynamic therapy of activated and resting lymphocytes and its antioxidant adaptive response
title_fullStr	Photodynamic therapy of activated and resting lymphocytes and its antioxidant adaptive response
title_full_unstemmed	Photodynamic therapy of activated and resting lymphocytes and its antioxidant adaptive response
title_sort	photodynamic therapy of activated and resting lymphocytes and its antioxidant adaptive response
publishDate	2002
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02688921_v17_n1_p42_Casas http://hdl.handle.net/20.500.12110/paper_02688921_v17_n1_p42_Casas
_version_	1768543466307977216

Photodynamic therapy of activated and resting lymphocytes and its antioxidant adaptive response

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