Betanin loaded nanocarriers based on quinoa seed 11S globulin. Impact on the protein structure and antioxidant activity

The objective of the present contribution was to design and characterize betanin (Bt) loaded 11S quinoa seed protein nanovehicles. 11S was isolated from quinoa seed floor. Protein purification was performed by Size-Exclusion Chromatography. MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization-Time...

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Detalles Bibliográficos
Publicado:	2019
Materias:	11S quinoa globulin Antioxidant activity Betanin Nanocarrier
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v87_n_p880_Martinez http://hdl.handle.net/20.500.12110/paper_0268005X_v87_n_p880_Martinez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0268005X_v87_n_p880_Martinez
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spelling	paper:paper_0268005X_v87_n_p880_Martinez2023-06-08T15:24:06Z Betanin loaded nanocarriers based on quinoa seed 11S globulin. Impact on the protein structure and antioxidant activity 11S quinoa globulin Antioxidant activity Betanin Nanocarrier The objective of the present contribution was to design and characterize betanin (Bt) loaded 11S quinoa seed protein nanovehicles. 11S was isolated from quinoa seed floor. Protein purification was performed by Size-Exclusion Chromatography. MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization-Time-Of- Flight) analysis confirmed the identity of 11S. Nanocarriers (11S-Bt) were generated at pH 8 at different ionic strength. Globulin intrinsic fluorescence spectra showed a quenching effect exerted by Bt, demonstrating in turn protein-bioactive interaction. Stern-Volmer and Scatchard models application confirmed static quenching and allowed to obtain parameters that described 11S and betanin complexation process. Bt-11S globulin interactions seem to be more probably of physical type. Protein solubility was increased after complexation with Bt. 11S betanin-loaded nanocarrier showed additive effect in terms of both, antiradical or reducing power capacity in comparison to Bt as evaluated by two methods, 2,2 -azino-bis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), and by ferric reducing antioxidant power (FRAP). Interestingly 11S globulin quaternary structure was modified by the bioactive, experimenting hexamer dissociation. This nanocarrier could have the potentiality to exert the Bt controlled delivery for pharmaceutical and nutraceutical products. Bt could also be protected from light and oxygen in such systems. © 2018 Elsevier Ltd 2019 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v87_n_p880_Martinez http://hdl.handle.net/20.500.12110/paper_0268005X_v87_n_p880_Martinez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	11S quinoa globulin Antioxidant activity Betanin Nanocarrier
spellingShingle	11S quinoa globulin Antioxidant activity Betanin Nanocarrier Betanin loaded nanocarriers based on quinoa seed 11S globulin. Impact on the protein structure and antioxidant activity
topic_facet	11S quinoa globulin Antioxidant activity Betanin Nanocarrier
description	The objective of the present contribution was to design and characterize betanin (Bt) loaded 11S quinoa seed protein nanovehicles. 11S was isolated from quinoa seed floor. Protein purification was performed by Size-Exclusion Chromatography. MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization-Time-Of- Flight) analysis confirmed the identity of 11S. Nanocarriers (11S-Bt) were generated at pH 8 at different ionic strength. Globulin intrinsic fluorescence spectra showed a quenching effect exerted by Bt, demonstrating in turn protein-bioactive interaction. Stern-Volmer and Scatchard models application confirmed static quenching and allowed to obtain parameters that described 11S and betanin complexation process. Bt-11S globulin interactions seem to be more probably of physical type. Protein solubility was increased after complexation with Bt. 11S betanin-loaded nanocarrier showed additive effect in terms of both, antiradical or reducing power capacity in comparison to Bt as evaluated by two methods, 2,2 -azino-bis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), and by ferric reducing antioxidant power (FRAP). Interestingly 11S globulin quaternary structure was modified by the bioactive, experimenting hexamer dissociation. This nanocarrier could have the potentiality to exert the Bt controlled delivery for pharmaceutical and nutraceutical products. Bt could also be protected from light and oxygen in such systems. © 2018 Elsevier Ltd
title	Betanin loaded nanocarriers based on quinoa seed 11S globulin. Impact on the protein structure and antioxidant activity
title_short	Betanin loaded nanocarriers based on quinoa seed 11S globulin. Impact on the protein structure and antioxidant activity
title_full	Betanin loaded nanocarriers based on quinoa seed 11S globulin. Impact on the protein structure and antioxidant activity
title_fullStr	Betanin loaded nanocarriers based on quinoa seed 11S globulin. Impact on the protein structure and antioxidant activity
title_full_unstemmed	Betanin loaded nanocarriers based on quinoa seed 11S globulin. Impact on the protein structure and antioxidant activity
title_sort	betanin loaded nanocarriers based on quinoa seed 11s globulin. impact on the protein structure and antioxidant activity
publishDate	2019
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v87_n_p880_Martinez http://hdl.handle.net/20.500.12110/paper_0268005X_v87_n_p880_Martinez
_version_	1768546539270045696

Betanin loaded nanocarriers based on quinoa seed 11S globulin. Impact on the protein structure and antioxidant activity

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