Hydroxypropylmethylcellulose-β-lactoglobulin mixtures at the oil-water interface. Bulk, interfacial and emulsification behavior as affected by pH

In this work we have studied the bulk, interfacial and emulsification behavior of a surface-active polysaccharide, hydroxypropylmethylcelulose (HPMC) and β-lactoglobulin (βlg) mixtures at two pHs, 3 and 6.The absence of an increase of particle size or a modification of zeta potential in HPMC/βlg mix...

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Detalles Bibliográficos
Autores principales: Camino, Nerina Andrea, Pilosof, Ana María Renata
Publicado: 2012
Materias:
β-Lactoglobulin
Emulsions
Hydroxypropylmethylcellulose
Interactions
Oil-water interface
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v27_n2_p464_Camino
http://hdl.handle.net/20.500.12110/paper_0268005X_v27_n2_p464_Camino
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_0268005X_v27_n2_p464_Camino
record_format dspace
spelling paper:paper_0268005X_v27_n2_p464_Camino2023-06-08T15:23:52Z Hydroxypropylmethylcellulose-β-lactoglobulin mixtures at the oil-water interface. Bulk, interfacial and emulsification behavior as affected by pH Camino, Nerina Andrea Pilosof, Ana María Renata β-Lactoglobulin Emulsions Hydroxypropylmethylcellulose Interactions Oil-water interface In this work we have studied the bulk, interfacial and emulsification behavior of a surface-active polysaccharide, hydroxypropylmethylcelulose (HPMC) and β-lactoglobulin (βlg) mixtures at two pHs, 3 and 6.The absence of an increase of particle size or a modification of zeta potential in HPMC/βlg mixtures indicated that both biopolymers would not form complexes in the bulk at pH 6. The protein presented a positive charge at pH 3, which makes possible the formation of a weak complex with the HPMC.The interface was dominated by the protein, indicating the displacement of the less surface-active HPMC at pH 6. The interfacial pressure of the HPMC/βlg mixture at pH 3 failed in between the curves corresponding to the single components βlg Complexation may decrease its availability to adsorb at the O/W interface, lowering the mixture interfacial pressure.A bimodal distribution was obtained in emulsions at pH 6 and their confocal images evidenced a strong flocculation, non-adsorbed HPMC would remain in the continuous phase, thus promoting depletion flocculation of oil droplets. At pH 3 the emulsion droplet size distribution curve was bimodal, indicating polidispersity. Nevertheless, a high stability to coalescence was observed. The mixed emulsion zeta potential is reduced in comparison with the protein alone, indicating complexation between βlg and HPMC. When analyzing the confocal microcopy images of recently prepared emulsions, no flocculation was observed. © 2011 Elsevier Ltd. Fil:Camino, N.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pilosof, A.M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v27_n2_p464_Camino http://hdl.handle.net/20.500.12110/paper_0268005X_v27_n2_p464_Camino
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic β-Lactoglobulin
Emulsions
Hydroxypropylmethylcellulose
Interactions
Oil-water interface
spellingShingle β-Lactoglobulin
Emulsions
Hydroxypropylmethylcellulose
Interactions
Oil-water interface
Camino, Nerina Andrea
Pilosof, Ana María Renata
Hydroxypropylmethylcellulose-β-lactoglobulin mixtures at the oil-water interface. Bulk, interfacial and emulsification behavior as affected by pH
topic_facet β-Lactoglobulin
Emulsions
Hydroxypropylmethylcellulose
Interactions
Oil-water interface
description In this work we have studied the bulk, interfacial and emulsification behavior of a surface-active polysaccharide, hydroxypropylmethylcelulose (HPMC) and β-lactoglobulin (βlg) mixtures at two pHs, 3 and 6.The absence of an increase of particle size or a modification of zeta potential in HPMC/βlg mixtures indicated that both biopolymers would not form complexes in the bulk at pH 6. The protein presented a positive charge at pH 3, which makes possible the formation of a weak complex with the HPMC.The interface was dominated by the protein, indicating the displacement of the less surface-active HPMC at pH 6. The interfacial pressure of the HPMC/βlg mixture at pH 3 failed in between the curves corresponding to the single components βlg Complexation may decrease its availability to adsorb at the O/W interface, lowering the mixture interfacial pressure.A bimodal distribution was obtained in emulsions at pH 6 and their confocal images evidenced a strong flocculation, non-adsorbed HPMC would remain in the continuous phase, thus promoting depletion flocculation of oil droplets. At pH 3 the emulsion droplet size distribution curve was bimodal, indicating polidispersity. Nevertheless, a high stability to coalescence was observed. The mixed emulsion zeta potential is reduced in comparison with the protein alone, indicating complexation between βlg and HPMC. When analyzing the confocal microcopy images of recently prepared emulsions, no flocculation was observed. © 2011 Elsevier Ltd.
author Camino, Nerina Andrea
Pilosof, Ana María Renata
author_facet Camino, Nerina Andrea
Pilosof, Ana María Renata
author_sort Camino, Nerina Andrea
title Hydroxypropylmethylcellulose-β-lactoglobulin mixtures at the oil-water interface. Bulk, interfacial and emulsification behavior as affected by pH
title_short Hydroxypropylmethylcellulose-β-lactoglobulin mixtures at the oil-water interface. Bulk, interfacial and emulsification behavior as affected by pH
title_full Hydroxypropylmethylcellulose-β-lactoglobulin mixtures at the oil-water interface. Bulk, interfacial and emulsification behavior as affected by pH
title_fullStr Hydroxypropylmethylcellulose-β-lactoglobulin mixtures at the oil-water interface. Bulk, interfacial and emulsification behavior as affected by pH
title_full_unstemmed Hydroxypropylmethylcellulose-β-lactoglobulin mixtures at the oil-water interface. Bulk, interfacial and emulsification behavior as affected by pH
title_sort hydroxypropylmethylcellulose-β-lactoglobulin mixtures at the oil-water interface. bulk, interfacial and emulsification behavior as affected by ph
publishDate 2012
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v27_n2_p464_Camino
http://hdl.handle.net/20.500.12110/paper_0268005X_v27_n2_p464_Camino
work_keys_str_mv AT caminonerinaandrea hydroxypropylmethylcelluloseblactoglobulinmixturesattheoilwaterinterfacebulkinterfacialandemulsificationbehaviorasaffectedbyph
AT pilosofanamariarenata hydroxypropylmethylcelluloseblactoglobulinmixturesattheoilwaterinterfacebulkinterfacialandemulsificationbehaviorasaffectedbyph
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