Soy protein-polysaccharides interactions at the air-water interface

This work studies the interfacial behavior of mixed soy protein (SP) + polysaccharide (PS) systems to gain knowledge on the interactions between these biopolymers at the air-water interface under dynamic conditions at neutral pH where a limited incompatibility between macromolecules can occur. The P...

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Autores principales: Martínez, Karina Dafne, Pilosof, Ana María Renata
Publicado: 2007
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v21_n5-6_p804_Martinez
http://hdl.handle.net/20.500.12110/paper_0268005X_v21_n5-6_p804_Martinez
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spelling paper:paper_0268005X_v21_n5-6_p804_Martinez2023-06-08T15:23:43Z Soy protein-polysaccharides interactions at the air-water interface Martínez, Karina Dafne Pilosof, Ana María Renata Dilatational rheology Gums Hydrocolloids Interactions Interface Soy protein Surface pressure Glycine max This work studies the interfacial behavior of mixed soy protein (SP) + polysaccharide (PS) systems to gain knowledge on the interactions between these biopolymers at the air-water interface under dynamic conditions at neutral pH where a limited incompatibility between macromolecules can occur. The PSs used were: hydroxypropylmethylcellulose (HPMC) as surface-active PS; lambda carrageenan (λ C) and locust bean (LB) gum as non-surface-active PSs. Protein and PS concentration in the mixed systems were 2% and 0.25%, respectively. The dynamic surface pressure and rheological properties of films were evaluated with a drop tensiometer at 20 {ring operator} C, pH 7 and ionic strength 0.05 M. The presence of HPMC and λ C greatly increased the surface pressure, surface dilatational elasticity and relative viscoelasticity on the basis of different mechanisms. HPMC competed for the interface with SP, but due to its unusual strong surface activity it could dominate the surface pressure and improve film viscoelasticity. The modification of surface pressure and rheological properties of adsorbed SP films in the presence of λ C necessarily suggests the participation of λ C + contaminants at the interface. Pure λ C could influence the interface by a complexation mechanism, or indirectly by a depletion mechanism in the vicinity of the interface. In addition surface-active contaminant of λ C if strongly bound to the PS could bring some PS molecules at the interface. LB little affected the surface pressure and rheological properties of SP films even if surface-active contaminants were present in the commercial preparation. Differences in the interaction of λ C and LB gum with the protein should be mainly ascribed to different degrees of incompatibility and to the fact that LB is not charged. © 2006 Elsevier Ltd. All rights reserved. Fil:Martinez, K.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pilosof, A.M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v21_n5-6_p804_Martinez http://hdl.handle.net/20.500.12110/paper_0268005X_v21_n5-6_p804_Martinez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Dilatational rheology
Gums
Hydrocolloids
Interactions
Interface
Soy protein
Surface pressure
Glycine max
spellingShingle Dilatational rheology
Gums
Hydrocolloids
Interactions
Interface
Soy protein
Surface pressure
Glycine max
Martínez, Karina Dafne
Pilosof, Ana María Renata
Soy protein-polysaccharides interactions at the air-water interface
topic_facet Dilatational rheology
Gums
Hydrocolloids
Interactions
Interface
Soy protein
Surface pressure
Glycine max
description This work studies the interfacial behavior of mixed soy protein (SP) + polysaccharide (PS) systems to gain knowledge on the interactions between these biopolymers at the air-water interface under dynamic conditions at neutral pH where a limited incompatibility between macromolecules can occur. The PSs used were: hydroxypropylmethylcellulose (HPMC) as surface-active PS; lambda carrageenan (λ C) and locust bean (LB) gum as non-surface-active PSs. Protein and PS concentration in the mixed systems were 2% and 0.25%, respectively. The dynamic surface pressure and rheological properties of films were evaluated with a drop tensiometer at 20 {ring operator} C, pH 7 and ionic strength 0.05 M. The presence of HPMC and λ C greatly increased the surface pressure, surface dilatational elasticity and relative viscoelasticity on the basis of different mechanisms. HPMC competed for the interface with SP, but due to its unusual strong surface activity it could dominate the surface pressure and improve film viscoelasticity. The modification of surface pressure and rheological properties of adsorbed SP films in the presence of λ C necessarily suggests the participation of λ C + contaminants at the interface. Pure λ C could influence the interface by a complexation mechanism, or indirectly by a depletion mechanism in the vicinity of the interface. In addition surface-active contaminant of λ C if strongly bound to the PS could bring some PS molecules at the interface. LB little affected the surface pressure and rheological properties of SP films even if surface-active contaminants were present in the commercial preparation. Differences in the interaction of λ C and LB gum with the protein should be mainly ascribed to different degrees of incompatibility and to the fact that LB is not charged. © 2006 Elsevier Ltd. All rights reserved.
author Martínez, Karina Dafne
Pilosof, Ana María Renata
author_facet Martínez, Karina Dafne
Pilosof, Ana María Renata
author_sort Martínez, Karina Dafne
title Soy protein-polysaccharides interactions at the air-water interface
title_short Soy protein-polysaccharides interactions at the air-water interface
title_full Soy protein-polysaccharides interactions at the air-water interface
title_fullStr Soy protein-polysaccharides interactions at the air-water interface
title_full_unstemmed Soy protein-polysaccharides interactions at the air-water interface
title_sort soy protein-polysaccharides interactions at the air-water interface
publishDate 2007
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v21_n5-6_p804_Martinez
http://hdl.handle.net/20.500.12110/paper_0268005X_v21_n5-6_p804_Martinez
work_keys_str_mv AT martinezkarinadafne soyproteinpolysaccharidesinteractionsattheairwaterinterface
AT pilosofanamariarenata soyproteinpolysaccharidesinteractionsattheairwaterinterface
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