Interactions of polysaccharides with β-lactoglobulin adsorbed films at the air-water interface

In the present work we have studied the adsorption (dynamic surface pressure) of β-lactoglobulin (βLG), polysaccharides and their mixtures at the air-water interface at 20°C and at pH 7. The measurements were performed on a automatic drop tensiometer. As polysaccharides with interfacial activity we...

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Autores principales:	Baeza, Rosa Isabel, Pilosof, Ana María Renata
Publicado:	2005
Materias:	Adsorbed film Air-water interface Gums Hydrocolloid Polysaccharide Protein Surface tension carrageenan lactoglobulin polysaccharide water xanthan adsorption air aqueous solution article chemical interaction esterification film hydrophilicity hydrophobicity molecular dynamics surface property thermodynamics viscosity
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v19_n2_p239_Baeza http://hdl.handle.net/20.500.12110/paper_0268005X_v19_n2_p239_Baeza
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0268005X_v19_n2_p239_Baeza
record_format	dspace
spelling	paper:paper_0268005X_v19_n2_p239_Baeza2023-06-08T15:23:42Z Interactions of polysaccharides with β-lactoglobulin adsorbed films at the air-water interface Baeza, Rosa Isabel Pilosof, Ana María Renata Adsorbed film Air-water interface Gums Hydrocolloid Polysaccharide Protein Surface tension carrageenan lactoglobulin polysaccharide water xanthan adsorption air aqueous solution article chemical interaction esterification film hydrophilicity hydrophobicity molecular dynamics surface property thermodynamics viscosity In the present work we have studied the adsorption (dynamic surface pressure) of β-lactoglobulin (βLG), polysaccharides and their mixtures at the air-water interface at 20°C and at pH 7. The measurements were performed on a automatic drop tensiometer. As polysaccharides with interfacial activity we have used propylene glycol alginates (PGA). To evaluate the effect of the degree of PGA esterification and viscosity, different commercial samples were studied - Kelcoloid O (KO), Kelcoloid LVF (KLVF) and Manucol ester (MAN). Xanthan gum (XG) and λ-carrageenan (λ-C) were studied as non-surface active polysaccharides. The results reveal a significant effect of surface-active and non-surface-active polysaccharides on dynamic characteristics of β-lactoglobulin adsorbed films. To explain the observed effects on the rates of diffusion, penetration, and rearrangement of these biopolymers at the air-water interface, three phenomena were taken into account: (i) the competitive adsorption; (ii) the complexation, and (iii) the existence of a limited thermodynamic compatibility between protein and polysaccharide at the air-water interface and in the bulk aqueous phase. Surface-active polysaccharides (MAN, KO) are less effective than non-adsorbing polysaccharides (XG) for increasing the surface pressure of protein films, because a competitive behaviour with protein. Highly hydrophilic polysaccharides that do not adsorb by their own at the interface (XG, λ-C) or surface-active polysaccharides with low hydrophobicity (KLVF) show a cooperative behaviour with protein that promotes a significant increase of surface pressure of adsorbed films. © 2004 Elsevier Ltd. All rights reserved. Fil:Baeza, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pilosof, A.M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v19_n2_p239_Baeza http://hdl.handle.net/20.500.12110/paper_0268005X_v19_n2_p239_Baeza
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Adsorbed film Air-water interface Gums Hydrocolloid Polysaccharide Protein Surface tension carrageenan lactoglobulin polysaccharide water xanthan adsorption air aqueous solution article chemical interaction esterification film hydrophilicity hydrophobicity molecular dynamics surface property thermodynamics viscosity
spellingShingle	Adsorbed film Air-water interface Gums Hydrocolloid Polysaccharide Protein Surface tension carrageenan lactoglobulin polysaccharide water xanthan adsorption air aqueous solution article chemical interaction esterification film hydrophilicity hydrophobicity molecular dynamics surface property thermodynamics viscosity Baeza, Rosa Isabel Pilosof, Ana María Renata Interactions of polysaccharides with β-lactoglobulin adsorbed films at the air-water interface
topic_facet	Adsorbed film Air-water interface Gums Hydrocolloid Polysaccharide Protein Surface tension carrageenan lactoglobulin polysaccharide water xanthan adsorption air aqueous solution article chemical interaction esterification film hydrophilicity hydrophobicity molecular dynamics surface property thermodynamics viscosity
description	In the present work we have studied the adsorption (dynamic surface pressure) of β-lactoglobulin (βLG), polysaccharides and their mixtures at the air-water interface at 20°C and at pH 7. The measurements were performed on a automatic drop tensiometer. As polysaccharides with interfacial activity we have used propylene glycol alginates (PGA). To evaluate the effect of the degree of PGA esterification and viscosity, different commercial samples were studied - Kelcoloid O (KO), Kelcoloid LVF (KLVF) and Manucol ester (MAN). Xanthan gum (XG) and λ-carrageenan (λ-C) were studied as non-surface active polysaccharides. The results reveal a significant effect of surface-active and non-surface-active polysaccharides on dynamic characteristics of β-lactoglobulin adsorbed films. To explain the observed effects on the rates of diffusion, penetration, and rearrangement of these biopolymers at the air-water interface, three phenomena were taken into account: (i) the competitive adsorption; (ii) the complexation, and (iii) the existence of a limited thermodynamic compatibility between protein and polysaccharide at the air-water interface and in the bulk aqueous phase. Surface-active polysaccharides (MAN, KO) are less effective than non-adsorbing polysaccharides (XG) for increasing the surface pressure of protein films, because a competitive behaviour with protein. Highly hydrophilic polysaccharides that do not adsorb by their own at the interface (XG, λ-C) or surface-active polysaccharides with low hydrophobicity (KLVF) show a cooperative behaviour with protein that promotes a significant increase of surface pressure of adsorbed films. © 2004 Elsevier Ltd. All rights reserved.
author	Baeza, Rosa Isabel Pilosof, Ana María Renata
author_facet	Baeza, Rosa Isabel Pilosof, Ana María Renata
author_sort	Baeza, Rosa Isabel
title	Interactions of polysaccharides with β-lactoglobulin adsorbed films at the air-water interface
title_short	Interactions of polysaccharides with β-lactoglobulin adsorbed films at the air-water interface
title_full	Interactions of polysaccharides with β-lactoglobulin adsorbed films at the air-water interface
title_fullStr	Interactions of polysaccharides with β-lactoglobulin adsorbed films at the air-water interface
title_full_unstemmed	Interactions of polysaccharides with β-lactoglobulin adsorbed films at the air-water interface
title_sort	interactions of polysaccharides with β-lactoglobulin adsorbed films at the air-water interface
publishDate	2005
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v19_n2_p239_Baeza http://hdl.handle.net/20.500.12110/paper_0268005X_v19_n2_p239_Baeza
work_keys_str_mv	AT baezarosaisabel interactionsofpolysaccharideswithblactoglobulinadsorbedfilmsattheairwaterinterface AT pilosofanamariarenata interactionsofpolysaccharideswithblactoglobulinadsorbedfilmsattheairwaterinterface
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Interactions of polysaccharides with β-lactoglobulin adsorbed films at the air-water interface

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