Interactions of polysaccharides with β-lactoglobulin spread monolayers at the air-water interface
In the present work we have studied the static (film structure and elasticity) and dynamic characteristics (surface dilatational properties) of β-lactoglobulin (βLG) monolayers spread at the air-water interface in the presence of polysaccharides in the aqueous phase, at 20°C and at pH 7. The measure...
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2004
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paper:paper_0268005X_v18_n6_p959_Baeza2023-06-08T15:23:42Z Interactions of polysaccharides with β-lactoglobulin spread monolayers at the air-water interface Baeza, Rosa Isabel Pilosof, Ana María Renata Air-water interface Gums Hydrocolloid Interfacial rheology Monolayer Polysaccharide Protein Surface tension alginic acid propylene glycol ester beta lactoglobulin carrageenan polysaccharide xanthan adsorption aqueous solution article esterification evaluation film flow kinetics isotherm molecular dynamics molecular interaction surface property thermodynamics viscoelasticity In the present work we have studied the static (film structure and elasticity) and dynamic characteristics (surface dilatational properties) of β-lactoglobulin (βLG) monolayers spread at the air-water interface in the presence of polysaccharides in the aqueous phase, at 20°C and at pH 7. The measurements were performed on a fully automated Wilhelmy-type film balance. As polysaccharides with interfacial activity we have used propylene glycol alginates (PGA). To evaluate the effect of the degree of PGA esterification and viscosity, different commercial samples were studied-kelcoloid O (KO), kelcoloid LVF (KLVF) and manucol ester (MAN). Xanthan gum (XG) and λ-carrageenan (λC) were studied as non-surface active polysaccharides. The results reveal a significant effect of surface active and non-surface active polysaccharides on static - when the polysaccharide was added in the subphase the π-A isotherms shifted to higher surface pressure values as the time increased-and dynamic - the presence of polysaccharide in the aqueous phase decreased the surface dilatational modulus of a pure β-lactoglobulin monolayer-characteristics of β-lactoglobulin monolayers. To explain the observed effects three phenomena were taken into account: (i) the ability of the polysaccharide to adsorb at the interface by it-self and to increase the surface pressure, (ii) the interfacial complexation of the polysaccharide with the adsorbed protein and (iii) the existence of a limited thermodynamic compatibility between the protein and polysaccharide, depending on the protein-polysaccharide system. © 2004 Elsevier Ltd. All rights reserved. Fil:Baeza, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pilosof, A.M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v18_n6_p959_Baeza http://hdl.handle.net/20.500.12110/paper_0268005X_v18_n6_p959_Baeza |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Air-water interface Gums Hydrocolloid Interfacial rheology Monolayer Polysaccharide Protein Surface tension alginic acid propylene glycol ester beta lactoglobulin carrageenan polysaccharide xanthan adsorption aqueous solution article esterification evaluation film flow kinetics isotherm molecular dynamics molecular interaction surface property thermodynamics viscoelasticity |
| spellingShingle |
Air-water interface Gums Hydrocolloid Interfacial rheology Monolayer Polysaccharide Protein Surface tension alginic acid propylene glycol ester beta lactoglobulin carrageenan polysaccharide xanthan adsorption aqueous solution article esterification evaluation film flow kinetics isotherm molecular dynamics molecular interaction surface property thermodynamics viscoelasticity Baeza, Rosa Isabel Pilosof, Ana María Renata Interactions of polysaccharides with β-lactoglobulin spread monolayers at the air-water interface |
| topic_facet |
Air-water interface Gums Hydrocolloid Interfacial rheology Monolayer Polysaccharide Protein Surface tension alginic acid propylene glycol ester beta lactoglobulin carrageenan polysaccharide xanthan adsorption aqueous solution article esterification evaluation film flow kinetics isotherm molecular dynamics molecular interaction surface property thermodynamics viscoelasticity |
| description |
In the present work we have studied the static (film structure and elasticity) and dynamic characteristics (surface dilatational properties) of β-lactoglobulin (βLG) monolayers spread at the air-water interface in the presence of polysaccharides in the aqueous phase, at 20°C and at pH 7. The measurements were performed on a fully automated Wilhelmy-type film balance. As polysaccharides with interfacial activity we have used propylene glycol alginates (PGA). To evaluate the effect of the degree of PGA esterification and viscosity, different commercial samples were studied-kelcoloid O (KO), kelcoloid LVF (KLVF) and manucol ester (MAN). Xanthan gum (XG) and λ-carrageenan (λC) were studied as non-surface active polysaccharides. The results reveal a significant effect of surface active and non-surface active polysaccharides on static - when the polysaccharide was added in the subphase the π-A isotherms shifted to higher surface pressure values as the time increased-and dynamic - the presence of polysaccharide in the aqueous phase decreased the surface dilatational modulus of a pure β-lactoglobulin monolayer-characteristics of β-lactoglobulin monolayers. To explain the observed effects three phenomena were taken into account: (i) the ability of the polysaccharide to adsorb at the interface by it-self and to increase the surface pressure, (ii) the interfacial complexation of the polysaccharide with the adsorbed protein and (iii) the existence of a limited thermodynamic compatibility between the protein and polysaccharide, depending on the protein-polysaccharide system. © 2004 Elsevier Ltd. All rights reserved. |
| author |
Baeza, Rosa Isabel Pilosof, Ana María Renata |
| author_facet |
Baeza, Rosa Isabel Pilosof, Ana María Renata |
| author_sort |
Baeza, Rosa Isabel |
| title |
Interactions of polysaccharides with β-lactoglobulin spread monolayers at the air-water interface |
| title_short |
Interactions of polysaccharides with β-lactoglobulin spread monolayers at the air-water interface |
| title_full |
Interactions of polysaccharides with β-lactoglobulin spread monolayers at the air-water interface |
| title_fullStr |
Interactions of polysaccharides with β-lactoglobulin spread monolayers at the air-water interface |
| title_full_unstemmed |
Interactions of polysaccharides with β-lactoglobulin spread monolayers at the air-water interface |
| title_sort |
interactions of polysaccharides with β-lactoglobulin spread monolayers at the air-water interface |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v18_n6_p959_Baeza http://hdl.handle.net/20.500.12110/paper_0268005X_v18_n6_p959_Baeza |
| work_keys_str_mv |
AT baezarosaisabel interactionsofpolysaccharideswithblactoglobulinspreadmonolayersattheairwaterinterface AT pilosofanamariarenata interactionsofpolysaccharideswithblactoglobulinspreadmonolayersattheairwaterinterface |
| _version_ |
1768546674039324672 |