Effects of gamma irradiation on bio-chemical and physico-chemical parameters of fresh-cut red beet (Beta vulgaris L. var. conditiva) root
Red beet (Beta vulgaris L. var. conditiva) root is a popular item present in ready to eat salads and minimally processed foods. In this research, the effect of low doses of gamma radiation (1 and 2 kGy) on peroxidase (POX), polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) activities, a...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02608774_v98_n2_p178_Latorre http://hdl.handle.net/20.500.12110/paper_02608774_v98_n2_p178_Latorre |
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paper:paper_02608774_v98_n2_p178_Latorre2023-06-08T15:22:43Z Effects of gamma irradiation on bio-chemical and physico-chemical parameters of fresh-cut red beet (Beta vulgaris L. var. conditiva) root Latorre, María Emilia Rojas, Ana María Luisa Gerschenson, Lía Noemí Bio-chemical parameters Enzymes Gamma irradiation Mechanical parameters Red beet Bio-chemical parameters Chemical parameters Gamma irradiation Mechanical parameters Red beet Adhesion Amino acids Calcium Catalysts Cell adhesion Cells Enzymes Gamma rays Industrial research Irradiation Plants (botany) Radiation Stresses Tissue Value engineering Chemical modification Beta vulgaris Beta vulgaris subsp. vulgaris Red beet (Beta vulgaris L. var. conditiva) root is a popular item present in ready to eat salads and minimally processed foods. In this research, the effect of low doses of gamma radiation (1 and 2 kGy) on peroxidase (POX), polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) activities, as well as on the levels of compounds related to the response to the oxidative stress of plant metabolism, the changes in colour and the mechanical behaviour of fresh-cut red beet root were analyzed, with the purpose of understanding the influence of the processing on tissue characteristics. Cell wall modifications were also studied through sequential extractions of polysaccharides from the alcohol-insoluble residues (AIR) obtained from each tissue. Irradiation seemed to contribute to higher cell-cell adhesion through increasing of calcium-cross linking at the middle lamellae regions, in addition to an increment of cross-links of polymers into the cell wall. Chemical modifications produced in the cell walls as a response to higher levels of H2O2 and subsequent POX mediated effects, were visualized structurally as a more elastic behaviour of irradiated tissues and rigidification of cell walls of treated roots, though puncture test did not reveal significant differences. Microscopy showed a continuum of thick cell walls in beet root tissue, which suffered slight modifications after irradiation, coherent with the bio-chemical results obtained. It can be concluded that irradiation doses of 1 or 2 kGy produced bio-chemical changes in cellular contents as well as in the cell wall constitutive networks which not necessarily could be sensed by consumers as it was objectively evaluated through a puncture test. At the same time, the mentioned changes involved an increase in the antioxidant capacity of red beet root tissue, showing that studied doses could be interesting to be used in the frame of a combined technique for red beet processing. © 2009 Elsevier Ltd. All rights reserved. Fil:Latorre, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rojas, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gerschenson, L.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02608774_v98_n2_p178_Latorre http://hdl.handle.net/20.500.12110/paper_02608774_v98_n2_p178_Latorre |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bio-chemical parameters Enzymes Gamma irradiation Mechanical parameters Red beet Bio-chemical parameters Chemical parameters Gamma irradiation Mechanical parameters Red beet Adhesion Amino acids Calcium Catalysts Cell adhesion Cells Enzymes Gamma rays Industrial research Irradiation Plants (botany) Radiation Stresses Tissue Value engineering Chemical modification Beta vulgaris Beta vulgaris subsp. vulgaris |
| spellingShingle |
Bio-chemical parameters Enzymes Gamma irradiation Mechanical parameters Red beet Bio-chemical parameters Chemical parameters Gamma irradiation Mechanical parameters Red beet Adhesion Amino acids Calcium Catalysts Cell adhesion Cells Enzymes Gamma rays Industrial research Irradiation Plants (botany) Radiation Stresses Tissue Value engineering Chemical modification Beta vulgaris Beta vulgaris subsp. vulgaris Latorre, María Emilia Rojas, Ana María Luisa Gerschenson, Lía Noemí Effects of gamma irradiation on bio-chemical and physico-chemical parameters of fresh-cut red beet (Beta vulgaris L. var. conditiva) root |
| topic_facet |
Bio-chemical parameters Enzymes Gamma irradiation Mechanical parameters Red beet Bio-chemical parameters Chemical parameters Gamma irradiation Mechanical parameters Red beet Adhesion Amino acids Calcium Catalysts Cell adhesion Cells Enzymes Gamma rays Industrial research Irradiation Plants (botany) Radiation Stresses Tissue Value engineering Chemical modification Beta vulgaris Beta vulgaris subsp. vulgaris |
| description |
Red beet (Beta vulgaris L. var. conditiva) root is a popular item present in ready to eat salads and minimally processed foods. In this research, the effect of low doses of gamma radiation (1 and 2 kGy) on peroxidase (POX), polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) activities, as well as on the levels of compounds related to the response to the oxidative stress of plant metabolism, the changes in colour and the mechanical behaviour of fresh-cut red beet root were analyzed, with the purpose of understanding the influence of the processing on tissue characteristics. Cell wall modifications were also studied through sequential extractions of polysaccharides from the alcohol-insoluble residues (AIR) obtained from each tissue. Irradiation seemed to contribute to higher cell-cell adhesion through increasing of calcium-cross linking at the middle lamellae regions, in addition to an increment of cross-links of polymers into the cell wall. Chemical modifications produced in the cell walls as a response to higher levels of H2O2 and subsequent POX mediated effects, were visualized structurally as a more elastic behaviour of irradiated tissues and rigidification of cell walls of treated roots, though puncture test did not reveal significant differences. Microscopy showed a continuum of thick cell walls in beet root tissue, which suffered slight modifications after irradiation, coherent with the bio-chemical results obtained. It can be concluded that irradiation doses of 1 or 2 kGy produced bio-chemical changes in cellular contents as well as in the cell wall constitutive networks which not necessarily could be sensed by consumers as it was objectively evaluated through a puncture test. At the same time, the mentioned changes involved an increase in the antioxidant capacity of red beet root tissue, showing that studied doses could be interesting to be used in the frame of a combined technique for red beet processing. © 2009 Elsevier Ltd. All rights reserved. |
| author |
Latorre, María Emilia Rojas, Ana María Luisa Gerschenson, Lía Noemí |
| author_facet |
Latorre, María Emilia Rojas, Ana María Luisa Gerschenson, Lía Noemí |
| author_sort |
Latorre, María Emilia |
| title |
Effects of gamma irradiation on bio-chemical and physico-chemical parameters of fresh-cut red beet (Beta vulgaris L. var. conditiva) root |
| title_short |
Effects of gamma irradiation on bio-chemical and physico-chemical parameters of fresh-cut red beet (Beta vulgaris L. var. conditiva) root |
| title_full |
Effects of gamma irradiation on bio-chemical and physico-chemical parameters of fresh-cut red beet (Beta vulgaris L. var. conditiva) root |
| title_fullStr |
Effects of gamma irradiation on bio-chemical and physico-chemical parameters of fresh-cut red beet (Beta vulgaris L. var. conditiva) root |
| title_full_unstemmed |
Effects of gamma irradiation on bio-chemical and physico-chemical parameters of fresh-cut red beet (Beta vulgaris L. var. conditiva) root |
| title_sort |
effects of gamma irradiation on bio-chemical and physico-chemical parameters of fresh-cut red beet (beta vulgaris l. var. conditiva) root |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02608774_v98_n2_p178_Latorre http://hdl.handle.net/20.500.12110/paper_02608774_v98_n2_p178_Latorre |
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