Commercial cell wall hydrolytic enzymes for producing pectin-enriched products from butternut (Cucurbita moschata, Duchesne ex Poiret)

A cell wall-enriched butternut (Cucurbita moschata Duch ex Poiret) powder was obtained by drying mesocarp wastes; this powder was used as the substrate for commercial cellulase (A-product) or hemicellulase (B-product) action. Yields were 4.70% and 6.12%, respectively. Both pectin-enriched products w...

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Autores principales: Fissore, Eliana Noemí, Stortz, Carlos Arturo, Gerschenson, Lía Noemí, Rojas, Ana María Luisa
Publicado: 2009
Materias:
Butternut
Composition
Enzymes
Pectin
Properties
Catalysts
Dialysis
Glucose
Molecular weight
Powders
Sugar (sucrose)
Average molecular weights
Cell walls
Chemical compositions
Cox-merz rules
Cucurbita moschata
Enhancing capacities
Flow behaviors
Galacturonic acids
Hemicellulase
Hydrolytic enzymes
Neutral sugars
Rhamnogalacturonan-I
Soluble fractions
Water-soluble fibers
Polysaccharides
Juglans cinerea
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02608774_v93_n3_p293_Fissore
http://hdl.handle.net/20.500.12110/paper_02608774_v93_n3_p293_Fissore
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_02608774_v93_n3_p293_Fissore
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spelling paper:paper_02608774_v93_n3_p293_Fissore2023-06-08T15:22:41Z Commercial cell wall hydrolytic enzymes for producing pectin-enriched products from butternut (Cucurbita moschata, Duchesne ex Poiret) Fissore, Eliana Noemí Stortz, Carlos Arturo Gerschenson, Lía Noemí Rojas, Ana María Luisa Butternut Composition Enzymes Pectin Properties Catalysts Dialysis Enzymes Glucose Molecular weight Powders Sugar (sucrose) Average molecular weights Butternut Cell walls Chemical compositions Composition Cox-merz rules Cucurbita moschata Enhancing capacities Flow behaviors Galacturonic acids Hemicellulase Hydrolytic enzymes Neutral sugars Pectin Properties Rhamnogalacturonan-I Soluble fractions Water-soluble fibers Polysaccharides Cucurbita moschata Juglans cinerea A cell wall-enriched butternut (Cucurbita moschata Duch ex Poiret) powder was obtained by drying mesocarp wastes; this powder was used as the substrate for commercial cellulase (A-product) or hemicellulase (B-product) action. Yields were 4.70% and 6.12%, respectively. Both pectin-enriched products were water soluble fibers and only the latter showed important thickening effect at 20 g dm-3-concentration in water. This product was a highly methylated (methylation degree, DM, 72.6) pectin with 45.2 g/100 g of galacturonic acid and 11 g/100 g of neutral sugars. Cellulase produced intense hydrolysis leading to a less methylated (DM: 54.2) A-fraction. It was observed that not only average molecular weight, but also the molecular structure derived from chemical composition affected flow behavior and viscosity enhancing capacity. As A-fraction was enriched in rhamnogalacturonan I, probably, rhamnose kinks were responsible for the poor thickening effect at 20 g dm-3-concentration in water, in spite of its homogeneous molecular weight profile, which showed only one peak at 793,000. This fraction presented the lowest density of macromolecular interactions in water, behaving as a diluted hydrocolloid solution as confirmed by its fitting to the Cox-Merz rule. Both isolated soluble fractions produced a delay in the glucose dialysis assay. © 2009 Elsevier Ltd. All rights reserved. Fil:Fissore, E.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stortz, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gerschenson, L.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rojas, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02608774_v93_n3_p293_Fissore http://hdl.handle.net/20.500.12110/paper_02608774_v93_n3_p293_Fissore
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Butternut
Composition
Enzymes
Pectin
Properties
Catalysts
Dialysis
Enzymes
Glucose
Molecular weight
Powders
Sugar (sucrose)
Average molecular weights
Butternut
Cell walls
Chemical compositions
Composition
Cox-merz rules
Cucurbita moschata
Enhancing capacities
Flow behaviors
Galacturonic acids
Hemicellulase
Hydrolytic enzymes
Neutral sugars
Pectin
Properties
Rhamnogalacturonan-I
Soluble fractions
Water-soluble fibers
Polysaccharides
Cucurbita moschata
Juglans cinerea
spellingShingle Butternut
Composition
Enzymes
Pectin
Properties
Catalysts
Dialysis
Enzymes
Glucose
Molecular weight
Powders
Sugar (sucrose)
Average molecular weights
Butternut
Cell walls
Chemical compositions
Composition
Cox-merz rules
Cucurbita moschata
Enhancing capacities
Flow behaviors
Galacturonic acids
Hemicellulase
Hydrolytic enzymes
Neutral sugars
Pectin
Properties
Rhamnogalacturonan-I
Soluble fractions
Water-soluble fibers
Polysaccharides
Cucurbita moschata
Juglans cinerea
Fissore, Eliana Noemí
Stortz, Carlos Arturo
Gerschenson, Lía Noemí
Rojas, Ana María Luisa
Commercial cell wall hydrolytic enzymes for producing pectin-enriched products from butternut (Cucurbita moschata, Duchesne ex Poiret)
topic_facet Butternut
Composition
Enzymes
Pectin
Properties
Catalysts
Dialysis
Enzymes
Glucose
Molecular weight
Powders
Sugar (sucrose)
Average molecular weights
Butternut
Cell walls
Chemical compositions
Composition
Cox-merz rules
Cucurbita moschata
Enhancing capacities
Flow behaviors
Galacturonic acids
Hemicellulase
Hydrolytic enzymes
Neutral sugars
Pectin
Properties
Rhamnogalacturonan-I
Soluble fractions
Water-soluble fibers
Polysaccharides
Cucurbita moschata
Juglans cinerea
description A cell wall-enriched butternut (Cucurbita moschata Duch ex Poiret) powder was obtained by drying mesocarp wastes; this powder was used as the substrate for commercial cellulase (A-product) or hemicellulase (B-product) action. Yields were 4.70% and 6.12%, respectively. Both pectin-enriched products were water soluble fibers and only the latter showed important thickening effect at 20 g dm-3-concentration in water. This product was a highly methylated (methylation degree, DM, 72.6) pectin with 45.2 g/100 g of galacturonic acid and 11 g/100 g of neutral sugars. Cellulase produced intense hydrolysis leading to a less methylated (DM: 54.2) A-fraction. It was observed that not only average molecular weight, but also the molecular structure derived from chemical composition affected flow behavior and viscosity enhancing capacity. As A-fraction was enriched in rhamnogalacturonan I, probably, rhamnose kinks were responsible for the poor thickening effect at 20 g dm-3-concentration in water, in spite of its homogeneous molecular weight profile, which showed only one peak at 793,000. This fraction presented the lowest density of macromolecular interactions in water, behaving as a diluted hydrocolloid solution as confirmed by its fitting to the Cox-Merz rule. Both isolated soluble fractions produced a delay in the glucose dialysis assay. © 2009 Elsevier Ltd. All rights reserved.
author Fissore, Eliana Noemí
Stortz, Carlos Arturo
Gerschenson, Lía Noemí
Rojas, Ana María Luisa
author_facet Fissore, Eliana Noemí
Stortz, Carlos Arturo
Gerschenson, Lía Noemí
Rojas, Ana María Luisa
author_sort Fissore, Eliana Noemí
title Commercial cell wall hydrolytic enzymes for producing pectin-enriched products from butternut (Cucurbita moschata, Duchesne ex Poiret)
title_short Commercial cell wall hydrolytic enzymes for producing pectin-enriched products from butternut (Cucurbita moschata, Duchesne ex Poiret)
title_full Commercial cell wall hydrolytic enzymes for producing pectin-enriched products from butternut (Cucurbita moschata, Duchesne ex Poiret)
title_fullStr Commercial cell wall hydrolytic enzymes for producing pectin-enriched products from butternut (Cucurbita moschata, Duchesne ex Poiret)
title_full_unstemmed Commercial cell wall hydrolytic enzymes for producing pectin-enriched products from butternut (Cucurbita moschata, Duchesne ex Poiret)
title_sort commercial cell wall hydrolytic enzymes for producing pectin-enriched products from butternut (cucurbita moschata, duchesne ex poiret)
publishDate 2009
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02608774_v93_n3_p293_Fissore
http://hdl.handle.net/20.500.12110/paper_02608774_v93_n3_p293_Fissore
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