β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation

The aim of this work was to build, to isolate and to characterize, core-shell microparticles composed of a core of thermally aggregated β-lactoglobulin (β-lg) covered by a shell of carboxymethylcellulose (CMC). The core-shell particles were obtained by mixing (β-lg)n and CMC solutions at pH 7 and fi...

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Autores principales: Martínez, María Julia, Pilosof, Ana María Renata, Pérez, Oscar E.
Publicado: 2014
Materias:
β-Lactoglobulin
Carboximethylcellulose
Core-shell microparticles
Electrostatic deposition
Lyophilization
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02608774_v131_n_p65_Carpineti
http://hdl.handle.net/20.500.12110/paper_02608774_v131_n_p65_Carpineti
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_02608774_v131_n_p65_Carpineti
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spelling paper:paper_02608774_v131_n_p65_Carpineti2025-07-30T18:00:38Z β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation Martínez, María Julia Pilosof, Ana María Renata Pérez, Oscar E. β-Lactoglobulin Carboximethylcellulose Core-shell microparticles Electrostatic deposition Lyophilization The aim of this work was to build, to isolate and to characterize, core-shell microparticles composed of a core of thermally aggregated β-lactoglobulin (β-lg) covered by a shell of carboxymethylcellulose (CMC). The core-shell particles were obtained by mixing (β-lg)n and CMC solutions at pH 7 and finally, decreasing the pH up to 4, promoting the adsorption of CMC on the protein core due their opposite electric charge. The core-shell microparticles were characterized by static laser light scattering (SLS), optical microscopy and atomic force microscopy (AFM). At pH 4, (β-lg)n showed a diameter ∼200 nm, but after adding the polysaccharide had a diameter ∼1 μm. The microscopy corroborated the data obtained by SLS measurements. Core-shell microparticles could be isolated by lyophilization and potentially applied as a fat replacement and/or a delivery systems for encapsulated substances in food formulations. © 2014 Elsevier Ltd. All rights reserved. Fil:Martinez, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pilosof, A.M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pérez, O.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02608774_v131_n_p65_Carpineti http://hdl.handle.net/20.500.12110/paper_02608774_v131_n_p65_Carpineti
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic β-Lactoglobulin
Carboximethylcellulose
Core-shell microparticles
Electrostatic deposition
Lyophilization
spellingShingle β-Lactoglobulin
Carboximethylcellulose
Core-shell microparticles
Electrostatic deposition
Lyophilization
Martínez, María Julia
Pilosof, Ana María Renata
Pérez, Oscar E.
β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation
topic_facet β-Lactoglobulin
Carboximethylcellulose
Core-shell microparticles
Electrostatic deposition
Lyophilization
description The aim of this work was to build, to isolate and to characterize, core-shell microparticles composed of a core of thermally aggregated β-lactoglobulin (β-lg) covered by a shell of carboxymethylcellulose (CMC). The core-shell particles were obtained by mixing (β-lg)n and CMC solutions at pH 7 and finally, decreasing the pH up to 4, promoting the adsorption of CMC on the protein core due their opposite electric charge. The core-shell microparticles were characterized by static laser light scattering (SLS), optical microscopy and atomic force microscopy (AFM). At pH 4, (β-lg)n showed a diameter ∼200 nm, but after adding the polysaccharide had a diameter ∼1 μm. The microscopy corroborated the data obtained by SLS measurements. Core-shell microparticles could be isolated by lyophilization and potentially applied as a fat replacement and/or a delivery systems for encapsulated substances in food formulations. © 2014 Elsevier Ltd. All rights reserved.
author Martínez, María Julia
Pilosof, Ana María Renata
Pérez, Oscar E.
author_facet Martínez, María Julia
Pilosof, Ana María Renata
Pérez, Oscar E.
author_sort Martínez, María Julia
title β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation
title_short β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation
title_full β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation
title_fullStr β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation
title_full_unstemmed β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation
title_sort β-lactoglobulin-carboxymethylcellulose core-shell microparticles: construction, characterization and isolation
publishDate 2014
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02608774_v131_n_p65_Carpineti
http://hdl.handle.net/20.500.12110/paper_02608774_v131_n_p65_Carpineti
work_keys_str_mv AT martinezmariajulia blactoglobulincarboxymethylcellulosecoreshellmicroparticlesconstructioncharacterizationandisolation
AT pilosofanamariarenata blactoglobulincarboxymethylcellulosecoreshellmicroparticlesconstructioncharacterizationandisolation
AT perezoscare blactoglobulincarboxymethylcellulosecoreshellmicroparticlesconstructioncharacterizationandisolation
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