A proteome map of a quadruple photoreceptor mutant sustains its severe photosynthetic deficient phenotype

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Light is the environmental factor that most affects plant growth and development through its impact on photomorphogenesis and photosynthesis. A quadruple photoreceptor mutant lacking four of the most important photoreceptors in plants, phytochromes A and B (phyA, phyB) and cryptochromes 1 and 2 (cry...

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Autores principales:	Fox, Ana Romina, Muschietti, Jorge P., Mazzella, María Agustina
Publicado:	2015
Materias:	Arabidopsis Cryptochrome Photosynthesis Phytochrome Proteome Arabidopsis protein proteome gene expression regulation genetics metabolism mutation photosynthesis radiation response two dimensional gel electrophoresis Arabidopsis Proteins Electrophoresis, Gel, Two-Dimensional Gene Expression Regulation, Plant Mutation
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01761617_v185_n_p13_Fox http://hdl.handle.net/20.500.12110/paper_01761617_v185_n_p13_Fox
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_01761617_v185_n_p13_Fox2023-06-08T15:19:05Z A proteome map of a quadruple photoreceptor mutant sustains its severe photosynthetic deficient phenotype Fox, Ana Romina Muschietti, Jorge P. Mazzella, María Agustina Arabidopsis Cryptochrome Photosynthesis Phytochrome Proteome Arabidopsis Arabidopsis protein proteome Arabidopsis gene expression regulation genetics metabolism mutation photosynthesis radiation response two dimensional gel electrophoresis Arabidopsis Arabidopsis Proteins Electrophoresis, Gel, Two-Dimensional Gene Expression Regulation, Plant Mutation Photosynthesis Proteome Light is the environmental factor that most affects plant growth and development through its impact on photomorphogenesis and photosynthesis. A quadruple photoreceptor mutant lacking four of the most important photoreceptors in plants, phytochromes A and B (phyA, phyB) and cryptochromes 1 and 2 (cry1, cry2), is severely affected in terms of growth and development. Previous studies have suggested that in addition to a photomorphogenic disorder, the phyA phyB cry1 cry2 quadruple mutant might have severe alterations in photosynthetic ability. Here, we investigated the photosynthetic processes altered in the quadruple mutant and performed a proteomic profiling approach to identify some of the proteins involved. The phyA phyB cry1 cry2 quadruple mutant showed reduced leaf area and total chlorophyll content. Photosynthetic rates at high irradiances were reduced approximately 65% compared to the wild type (WT). Light-saturated photosynthesis and the response of net CO<inf>2</inf> exchange to low and high internal CO<inf>2</inf> concentrations suggest that the levels or activity of the components of the Calvin cycle and electron transport might be reduced in the quadruple mutant. Most of the under-expressed proteins in the phyA phyB cry1 cry2 quadruple mutant consistently showed a chloroplastic localization, whereas components of the Calvin cycle and light reaction centers were overrepresented. Additionally, Rubisco expression was reduced threefold in the phyA phyB cry1 cry2 quadruple mutant.Together, these results highlight the importance of the phytochrome and cryptochrome families in proper autotrophy establishment in plants. They also suggest that an overall limitation in the chlorophyll levels, expression of Rubisco, and enzymes of the Calvin Cycle and electron transport that affect ribulose-1,5-biphosphate (RuBP) regeneration reduced photosynthetic capacity in the phyA phyB cry1 cry2 quadruple mutant. © 2015 Elsevier GmbH. Fil:Fox, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muschietti, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mazzella, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01761617_v185_n_p13_Fox http://hdl.handle.net/20.500.12110/paper_01761617_v185_n_p13_Fox
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Arabidopsis Cryptochrome Photosynthesis Phytochrome Proteome Arabidopsis Arabidopsis protein proteome Arabidopsis gene expression regulation genetics metabolism mutation photosynthesis radiation response two dimensional gel electrophoresis Arabidopsis Arabidopsis Proteins Electrophoresis, Gel, Two-Dimensional Gene Expression Regulation, Plant Mutation Photosynthesis Proteome
spellingShingle	Arabidopsis Cryptochrome Photosynthesis Phytochrome Proteome Arabidopsis Arabidopsis protein proteome Arabidopsis gene expression regulation genetics metabolism mutation photosynthesis radiation response two dimensional gel electrophoresis Arabidopsis Arabidopsis Proteins Electrophoresis, Gel, Two-Dimensional Gene Expression Regulation, Plant Mutation Photosynthesis Proteome Fox, Ana Romina Muschietti, Jorge P. Mazzella, María Agustina A proteome map of a quadruple photoreceptor mutant sustains its severe photosynthetic deficient phenotype
topic_facet	Arabidopsis Cryptochrome Photosynthesis Phytochrome Proteome Arabidopsis Arabidopsis protein proteome Arabidopsis gene expression regulation genetics metabolism mutation photosynthesis radiation response two dimensional gel electrophoresis Arabidopsis Arabidopsis Proteins Electrophoresis, Gel, Two-Dimensional Gene Expression Regulation, Plant Mutation Photosynthesis Proteome
description	Light is the environmental factor that most affects plant growth and development through its impact on photomorphogenesis and photosynthesis. A quadruple photoreceptor mutant lacking four of the most important photoreceptors in plants, phytochromes A and B (phyA, phyB) and cryptochromes 1 and 2 (cry1, cry2), is severely affected in terms of growth and development. Previous studies have suggested that in addition to a photomorphogenic disorder, the phyA phyB cry1 cry2 quadruple mutant might have severe alterations in photosynthetic ability. Here, we investigated the photosynthetic processes altered in the quadruple mutant and performed a proteomic profiling approach to identify some of the proteins involved. The phyA phyB cry1 cry2 quadruple mutant showed reduced leaf area and total chlorophyll content. Photosynthetic rates at high irradiances were reduced approximately 65% compared to the wild type (WT). Light-saturated photosynthesis and the response of net CO<inf>2</inf> exchange to low and high internal CO<inf>2</inf> concentrations suggest that the levels or activity of the components of the Calvin cycle and electron transport might be reduced in the quadruple mutant. Most of the under-expressed proteins in the phyA phyB cry1 cry2 quadruple mutant consistently showed a chloroplastic localization, whereas components of the Calvin cycle and light reaction centers were overrepresented. Additionally, Rubisco expression was reduced threefold in the phyA phyB cry1 cry2 quadruple mutant.Together, these results highlight the importance of the phytochrome and cryptochrome families in proper autotrophy establishment in plants. They also suggest that an overall limitation in the chlorophyll levels, expression of Rubisco, and enzymes of the Calvin Cycle and electron transport that affect ribulose-1,5-biphosphate (RuBP) regeneration reduced photosynthetic capacity in the phyA phyB cry1 cry2 quadruple mutant. © 2015 Elsevier GmbH.
author	Fox, Ana Romina Muschietti, Jorge P. Mazzella, María Agustina
author_facet	Fox, Ana Romina Muschietti, Jorge P. Mazzella, María Agustina
author_sort	Fox, Ana Romina
title	A proteome map of a quadruple photoreceptor mutant sustains its severe photosynthetic deficient phenotype
title_short	A proteome map of a quadruple photoreceptor mutant sustains its severe photosynthetic deficient phenotype
title_full	A proteome map of a quadruple photoreceptor mutant sustains its severe photosynthetic deficient phenotype
title_fullStr	A proteome map of a quadruple photoreceptor mutant sustains its severe photosynthetic deficient phenotype
title_full_unstemmed	A proteome map of a quadruple photoreceptor mutant sustains its severe photosynthetic deficient phenotype
title_sort	proteome map of a quadruple photoreceptor mutant sustains its severe photosynthetic deficient phenotype
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01761617_v185_n_p13_Fox http://hdl.handle.net/20.500.12110/paper_01761617_v185_n_p13_Fox
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_version_	1768544176937369600

A proteome map of a quadruple photoreceptor mutant sustains its severe photosynthetic deficient phenotype

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