Pregnenolone and progesterone metabolism by the testes of Bufo arenarum
Sliced testis tissue from Bufo arenarum was incubated in the presence of [3H]pregnenolone. Testis fragments were also used for double isotope experiments using [3H]pregnenolone and [14C]progesterone. Specific activities were equated with the addition of radioinert pregnenolone. When yields of radiom...
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1998
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01741578_v168_n7_p491_Canosa http://hdl.handle.net/20.500.12110/paper_01741578_v168_n7_p491_Canosa |
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paper:paper_01741578_v168_n7_p491_Canosa2023-06-08T15:18:53Z Pregnenolone and progesterone metabolism by the testes of Bufo arenarum Anura Steroid hormones Steroidogenesis Testes Toad Bufo arenarum Sliced testis tissue from Bufo arenarum was incubated in the presence of [3H]pregnenolone. Testis fragments were also used for double isotope experiments using [3H]pregnenolone and [14C]progesterone. Specific activities were equated with the addition of radioinert pregnenolone. When yields of radiometabolites were analysed, pregnenolone was found to be a good precursor for C19 steroids such as dehydroepiandrosterone, 5-androsten-3β,17β diol, testosterone, 5α-dihydrotestosterone and a C21 steroid, 5α-pregnan-3,20 dione. Progesterone mainly converts to 5α-pregnan-3,20 dione, asteroid with unknown function in amphibians. The 5-ene pathway, including 5-androsten-3β,17β diol as intermediate, could be predominant for androgen biosynthesis. Testes bypass not only progesterone but also androstenedione for testosterone biosynthesis. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01741578_v168_n7_p491_Canosa http://hdl.handle.net/20.500.12110/paper_01741578_v168_n7_p491_Canosa |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Anura Steroid hormones Steroidogenesis Testes Toad Bufo arenarum |
| spellingShingle |
Anura Steroid hormones Steroidogenesis Testes Toad Bufo arenarum Pregnenolone and progesterone metabolism by the testes of Bufo arenarum |
| topic_facet |
Anura Steroid hormones Steroidogenesis Testes Toad Bufo arenarum |
| description |
Sliced testis tissue from Bufo arenarum was incubated in the presence of [3H]pregnenolone. Testis fragments were also used for double isotope experiments using [3H]pregnenolone and [14C]progesterone. Specific activities were equated with the addition of radioinert pregnenolone. When yields of radiometabolites were analysed, pregnenolone was found to be a good precursor for C19 steroids such as dehydroepiandrosterone, 5-androsten-3β,17β diol, testosterone, 5α-dihydrotestosterone and a C21 steroid, 5α-pregnan-3,20 dione. Progesterone mainly converts to 5α-pregnan-3,20 dione, asteroid with unknown function in amphibians. The 5-ene pathway, including 5-androsten-3β,17β diol as intermediate, could be predominant for androgen biosynthesis. Testes bypass not only progesterone but also androstenedione for testosterone biosynthesis. |
| title |
Pregnenolone and progesterone metabolism by the testes of Bufo arenarum |
| title_short |
Pregnenolone and progesterone metabolism by the testes of Bufo arenarum |
| title_full |
Pregnenolone and progesterone metabolism by the testes of Bufo arenarum |
| title_fullStr |
Pregnenolone and progesterone metabolism by the testes of Bufo arenarum |
| title_full_unstemmed |
Pregnenolone and progesterone metabolism by the testes of Bufo arenarum |
| title_sort |
pregnenolone and progesterone metabolism by the testes of bufo arenarum |
| publishDate |
1998 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01741578_v168_n7_p491_Canosa http://hdl.handle.net/20.500.12110/paper_01741578_v168_n7_p491_Canosa |
| _version_ |
1768546719985827840 |