Selection of an extracellular esterase-producing microorganism

The conventional esterase plate-screening technique has been modified in order to obtain a better differentiation of high producing strains. A short exposure to Lugol's iodine solution after colony growth enhanced the contrast between the precipitation halo and the background. Batch cultures of...

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Detalles Bibliográficos
Autores principales:	Castro, Guillermo Raúl, Ferrero, Marcela Alejandra, Siñeriz, Faustino
Publicado:	1992
Materias:	B. subtilis esterase Esterase production Esterase screening test pH profile Temperature profile Batch cell culture Biotechnology Enzymes Microbiology Esterase plate screening Esterase producing microorganisms Extracellular esterases Microorganisms esterase article bacillus subtilis microorganism nonhuman ph priority journal screening temperature
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01694146_v10_n3-4_p165_Castro http://hdl.handle.net/20.500.12110/paper_01694146_v10_n3-4_p165_Castro
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01694146_v10_n3-4_p165_Castro
record_format	dspace
spelling	paper:paper_01694146_v10_n3-4_p165_Castro2023-06-08T15:18:19Z Selection of an extracellular esterase-producing microorganism Castro, Guillermo Raúl Ferrero, Marcela Alejandra Siñeriz, Faustino B. subtilis esterase Esterase production Esterase screening test pH profile Temperature profile Batch cell culture Biotechnology Enzymes Microbiology Esterase plate screening Esterase producing microorganisms Extracellular esterases Microorganisms esterase article bacillus subtilis microorganism nonhuman ph priority journal screening temperature The conventional esterase plate-screening technique has been modified in order to obtain a better differentiation of high producing strains. A short exposure to Lugol's iodine solution after colony growth enhanced the contrast between the precipitation halo and the background. Batch cultures of a selected strain characterized as belonging to the Bacillus subtilis group showed a high level of extracellular activity at pH 6.6 to 8.0 and 35°C. In crude extracts the optimum enzymatic activity was obtained at 35°C and pH 7.0. © 1992 Society for Industrial Microbiology. Fil:Castro, G.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ferrero, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Siñeriz, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1992 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01694146_v10_n3-4_p165_Castro http://hdl.handle.net/20.500.12110/paper_01694146_v10_n3-4_p165_Castro
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	B. subtilis esterase Esterase production Esterase screening test pH profile Temperature profile Batch cell culture Biotechnology Enzymes Microbiology Esterase plate screening Esterase producing microorganisms Extracellular esterases Microorganisms esterase article bacillus subtilis microorganism nonhuman ph priority journal screening temperature
spellingShingle	B. subtilis esterase Esterase production Esterase screening test pH profile Temperature profile Batch cell culture Biotechnology Enzymes Microbiology Esterase plate screening Esterase producing microorganisms Extracellular esterases Microorganisms esterase article bacillus subtilis microorganism nonhuman ph priority journal screening temperature Castro, Guillermo Raúl Ferrero, Marcela Alejandra Siñeriz, Faustino Selection of an extracellular esterase-producing microorganism
topic_facet	B. subtilis esterase Esterase production Esterase screening test pH profile Temperature profile Batch cell culture Biotechnology Enzymes Microbiology Esterase plate screening Esterase producing microorganisms Extracellular esterases Microorganisms esterase article bacillus subtilis microorganism nonhuman ph priority journal screening temperature
description	The conventional esterase plate-screening technique has been modified in order to obtain a better differentiation of high producing strains. A short exposure to Lugol's iodine solution after colony growth enhanced the contrast between the precipitation halo and the background. Batch cultures of a selected strain characterized as belonging to the Bacillus subtilis group showed a high level of extracellular activity at pH 6.6 to 8.0 and 35°C. In crude extracts the optimum enzymatic activity was obtained at 35°C and pH 7.0. © 1992 Society for Industrial Microbiology.
author	Castro, Guillermo Raúl Ferrero, Marcela Alejandra Siñeriz, Faustino
author_facet	Castro, Guillermo Raúl Ferrero, Marcela Alejandra Siñeriz, Faustino
author_sort	Castro, Guillermo Raúl
title	Selection of an extracellular esterase-producing microorganism
title_short	Selection of an extracellular esterase-producing microorganism
title_full	Selection of an extracellular esterase-producing microorganism
title_fullStr	Selection of an extracellular esterase-producing microorganism
title_full_unstemmed	Selection of an extracellular esterase-producing microorganism
title_sort	selection of an extracellular esterase-producing microorganism
publishDate	1992
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01694146_v10_n3-4_p165_Castro http://hdl.handle.net/20.500.12110/paper_01694146_v10_n3-4_p165_Castro
work_keys_str_mv	AT castroguillermoraul selectionofanextracellularesteraseproducingmicroorganism AT ferreromarcelaalejandra selectionofanextracellularesteraseproducingmicroorganism AT sinerizfaustino selectionofanextracellularesteraseproducingmicroorganism
_version_	1768545001119154176

Selection of an extracellular esterase-producing microorganism

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