Correlation between synaptogenesis and the PTEN phosphatase expression in dendrites during postnatal brain development
The PTEN (phosphatase and tensin homolog deleted on chromosome 10) tumor suppressor gene codifies a lipid inositol 3′-phosphatase that negatively regulates cell survival mediated by the phosphatidyl inositol 3′ kinase (PIP3-kinase)-protein kinase B/Akt signaling pathway. Recently, PIP3-kinase was in...
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2004
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0169328X_v128_n1_p8_Perandones http://hdl.handle.net/20.500.12110/paper_0169328X_v128_n1_p8_Perandones |
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paper:paper_0169328X_v128_n1_p8_Perandones2023-06-08T15:18:17Z Correlation between synaptogenesis and the PTEN phosphatase expression in dendrites during postnatal brain development Costanzo, Roxana V. Carminatti, Héctor Radrizzani Helguera, Martín Cellular and molecular biology Cerebellum Dendrites Phosphatidylinositol phosphatase PTEN Staining, tracing, and imaging techniques Synaptogenesis Weaver phosphatase phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase animal cell animal tissue article brain cortex brain development cell survival cerebellum controlled study correlation analysis dendrite granule cell hippocampus mouse nerve fiber nonhuman olfactory bulb polarization postnatal development priority journal protein analysis protein expression Purkinje cell sequence homology synaptogenesis 1-Phosphatidylinositol 3-Kinase Animals Brain Catalytic Domain Cell Polarity Dendrites Immunohistochemistry Mice Mice, Inbred C57BL Mice, Neurologic Mutants Peptide Fragments Protein-Serine-Threonine Kinases Protein-Tyrosine-Phosphatase Proto-Oncogene Proteins Proto-Oncogene Proteins c-akt PTEN Phosphohydrolase Signal Transduction Statistics Synapses Tissue Distribution Tumor Suppressor Proteins The PTEN (phosphatase and tensin homolog deleted on chromosome 10) tumor suppressor gene codifies a lipid inositol 3′-phosphatase that negatively regulates cell survival mediated by the phosphatidyl inositol 3′ kinase (PIP3-kinase)-protein kinase B/Akt signaling pathway. Recently, PIP3-kinase was involved in axon polarization, but PTEN functions in dendrites are uncertain. Using amino-terminal antibodies against the catalytic domain, we found a 34 kDa fragment of PTEN protein detected only in mouse brain tissue, present in neuron dendrites and spines of cerebral cortex, cerebellum, hippocampus and olfactory bulb. The PTEN-fragment reaches the synaptic fraction with a positive temporal correlation with synaptic stabilization in postnatal cerebellum and brain. In the weaver mutant mice, PTEN was absent only in the Purkinje cells dendrites that cannot receive the granule cells synaptic input. Furthermore, the activated p-Akt/PKB was present in axons but not in dendrites of mature neuron cells. P-Akt was also altered by the weaver mutation maintaining the inverse correlation with the PTEN-fragment in Purkinje cell dendrites. In contrast, the expression of this fragment was not affected by the staggerer mutation. Together, these results suggest that synaptogenesis is a necessary process for polarization in PIP3 pathway mediated by the PTEN catalytic-fragment into dendrites of CNS neurons. © 2004 Elsevier B.V. All rights reserved. Fil:Costanzo, R.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carminatti, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Radrizzani, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0169328X_v128_n1_p8_Perandones http://hdl.handle.net/20.500.12110/paper_0169328X_v128_n1_p8_Perandones |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cellular and molecular biology Cerebellum Dendrites Phosphatidylinositol phosphatase PTEN Staining, tracing, and imaging techniques Synaptogenesis Weaver phosphatase phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase animal cell animal tissue article brain cortex brain development cell survival cerebellum controlled study correlation analysis dendrite granule cell hippocampus mouse nerve fiber nonhuman olfactory bulb polarization postnatal development priority journal protein analysis protein expression Purkinje cell sequence homology synaptogenesis 1-Phosphatidylinositol 3-Kinase Animals Brain Catalytic Domain Cell Polarity Dendrites Immunohistochemistry Mice Mice, Inbred C57BL Mice, Neurologic Mutants Peptide Fragments Protein-Serine-Threonine Kinases Protein-Tyrosine-Phosphatase Proto-Oncogene Proteins Proto-Oncogene Proteins c-akt PTEN Phosphohydrolase Signal Transduction Statistics Synapses Tissue Distribution Tumor Suppressor Proteins |
| spellingShingle |
Cellular and molecular biology Cerebellum Dendrites Phosphatidylinositol phosphatase PTEN Staining, tracing, and imaging techniques Synaptogenesis Weaver phosphatase phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase animal cell animal tissue article brain cortex brain development cell survival cerebellum controlled study correlation analysis dendrite granule cell hippocampus mouse nerve fiber nonhuman olfactory bulb polarization postnatal development priority journal protein analysis protein expression Purkinje cell sequence homology synaptogenesis 1-Phosphatidylinositol 3-Kinase Animals Brain Catalytic Domain Cell Polarity Dendrites Immunohistochemistry Mice Mice, Inbred C57BL Mice, Neurologic Mutants Peptide Fragments Protein-Serine-Threonine Kinases Protein-Tyrosine-Phosphatase Proto-Oncogene Proteins Proto-Oncogene Proteins c-akt PTEN Phosphohydrolase Signal Transduction Statistics Synapses Tissue Distribution Tumor Suppressor Proteins Costanzo, Roxana V. Carminatti, Héctor Radrizzani Helguera, Martín Correlation between synaptogenesis and the PTEN phosphatase expression in dendrites during postnatal brain development |
| topic_facet |
Cellular and molecular biology Cerebellum Dendrites Phosphatidylinositol phosphatase PTEN Staining, tracing, and imaging techniques Synaptogenesis Weaver phosphatase phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase animal cell animal tissue article brain cortex brain development cell survival cerebellum controlled study correlation analysis dendrite granule cell hippocampus mouse nerve fiber nonhuman olfactory bulb polarization postnatal development priority journal protein analysis protein expression Purkinje cell sequence homology synaptogenesis 1-Phosphatidylinositol 3-Kinase Animals Brain Catalytic Domain Cell Polarity Dendrites Immunohistochemistry Mice Mice, Inbred C57BL Mice, Neurologic Mutants Peptide Fragments Protein-Serine-Threonine Kinases Protein-Tyrosine-Phosphatase Proto-Oncogene Proteins Proto-Oncogene Proteins c-akt PTEN Phosphohydrolase Signal Transduction Statistics Synapses Tissue Distribution Tumor Suppressor Proteins |
| description |
The PTEN (phosphatase and tensin homolog deleted on chromosome 10) tumor suppressor gene codifies a lipid inositol 3′-phosphatase that negatively regulates cell survival mediated by the phosphatidyl inositol 3′ kinase (PIP3-kinase)-protein kinase B/Akt signaling pathway. Recently, PIP3-kinase was involved in axon polarization, but PTEN functions in dendrites are uncertain. Using amino-terminal antibodies against the catalytic domain, we found a 34 kDa fragment of PTEN protein detected only in mouse brain tissue, present in neuron dendrites and spines of cerebral cortex, cerebellum, hippocampus and olfactory bulb. The PTEN-fragment reaches the synaptic fraction with a positive temporal correlation with synaptic stabilization in postnatal cerebellum and brain. In the weaver mutant mice, PTEN was absent only in the Purkinje cells dendrites that cannot receive the granule cells synaptic input. Furthermore, the activated p-Akt/PKB was present in axons but not in dendrites of mature neuron cells. P-Akt was also altered by the weaver mutation maintaining the inverse correlation with the PTEN-fragment in Purkinje cell dendrites. In contrast, the expression of this fragment was not affected by the staggerer mutation. Together, these results suggest that synaptogenesis is a necessary process for polarization in PIP3 pathway mediated by the PTEN catalytic-fragment into dendrites of CNS neurons. © 2004 Elsevier B.V. All rights reserved. |
| author |
Costanzo, Roxana V. Carminatti, Héctor Radrizzani Helguera, Martín |
| author_facet |
Costanzo, Roxana V. Carminatti, Héctor Radrizzani Helguera, Martín |
| author_sort |
Costanzo, Roxana V. |
| title |
Correlation between synaptogenesis and the PTEN phosphatase expression in dendrites during postnatal brain development |
| title_short |
Correlation between synaptogenesis and the PTEN phosphatase expression in dendrites during postnatal brain development |
| title_full |
Correlation between synaptogenesis and the PTEN phosphatase expression in dendrites during postnatal brain development |
| title_fullStr |
Correlation between synaptogenesis and the PTEN phosphatase expression in dendrites during postnatal brain development |
| title_full_unstemmed |
Correlation between synaptogenesis and the PTEN phosphatase expression in dendrites during postnatal brain development |
| title_sort |
correlation between synaptogenesis and the pten phosphatase expression in dendrites during postnatal brain development |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0169328X_v128_n1_p8_Perandones http://hdl.handle.net/20.500.12110/paper_0169328X_v128_n1_p8_Perandones |
| work_keys_str_mv |
AT costanzoroxanav correlationbetweensynaptogenesisandtheptenphosphataseexpressionindendritesduringpostnatalbraindevelopment AT carminattihector correlationbetweensynaptogenesisandtheptenphosphataseexpressionindendritesduringpostnatalbraindevelopment AT radrizzanihelgueramartin correlationbetweensynaptogenesisandtheptenphosphataseexpressionindendritesduringpostnatalbraindevelopment |
| _version_ |
1768543129920602112 |