How Are Short Exons Flanked by Long Introns Defined and Committed to Splicing?

The splice sites (SSs) delimiting an intron are brought together in the earliest step of spliceosome assembly yet it remains obscure how SS pairing occurs, especially when introns are thousands of nucleotides long. Splicing occurs in vivo in mammals within minutes regardless of intron length, implyi...

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Autor principal:	Kornblihtt, Alberto Rodolfo
Publicado:	2016
Materias:	RNA polymerase II small nuclear ribonucleoprotein splicing factor U2AF U2AF2 protein, human carboxy terminal sequence chromatin chromatin structure exon genetic model genetic transcription human in vivo study intron kinetics length molecular evolution priority journal Review RNA binding RNA splicing RNA synthesis spliceosome genetics Chromatin Exons Humans Introns Ribonucleoprotein, U1 Small Nuclear Ribonucleoproteins, Small Nuclear RNA Polymerase II RNA Splicing Spliceosomes Splicing Factor U2AF
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689525_v32_n10_p596_Hollander http://hdl.handle.net/20.500.12110/paper_01689525_v32_n10_p596_Hollander
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01689525_v32_n10_p596_Hollander
record_format	dspace
spelling	paper:paper_01689525_v32_n10_p596_Hollander2023-06-08T15:18:10Z How Are Short Exons Flanked by Long Introns Defined and Committed to Splicing? Kornblihtt, Alberto Rodolfo RNA polymerase II small nuclear ribonucleoprotein RNA polymerase II small nuclear ribonucleoprotein splicing factor U2AF U2AF2 protein, human carboxy terminal sequence chromatin chromatin structure exon genetic model genetic transcription human in vivo study intron kinetics length molecular evolution priority journal Review RNA binding RNA splicing RNA synthesis spliceosome exon genetics intron RNA splicing Chromatin Exons Humans Introns Ribonucleoprotein, U1 Small Nuclear Ribonucleoproteins, Small Nuclear RNA Polymerase II RNA Splicing Spliceosomes Splicing Factor U2AF The splice sites (SSs) delimiting an intron are brought together in the earliest step of spliceosome assembly yet it remains obscure how SS pairing occurs, especially when introns are thousands of nucleotides long. Splicing occurs in vivo in mammals within minutes regardless of intron length, implying that SS pairing can instantly follow transcription. Also, factors required for SS pairing, such as the U1 small nuclear ribonucleoprotein (snRNP) and U2AF65, associate with RNA polymerase II (RNAPII), while nucleosomes preferentially bind exonic sequences and associate with U2 snRNP. Based on recent publications, we assume that the 5′ SS-bound U1 snRNP can remain tethered to RNAPII until complete synthesis of the downstream intron and exon. An additional U1 snRNP then binds the downstream 5′ SS, whereas the RNAPII-associated U2AF65 binds the upstream 3′ SS to facilitate SS pairing along with exon definition. Next, the nucleosome-associated U2 snRNP binds the branch site to advance splicing complex assembly. This may explain how RNAPII and chromatin are involved in spliceosome assembly and how introns lengthened during evolution with a relatively minimal compromise in splicing. © 2016 Elsevier Ltd Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689525_v32_n10_p596_Hollander http://hdl.handle.net/20.500.12110/paper_01689525_v32_n10_p596_Hollander
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	RNA polymerase II small nuclear ribonucleoprotein RNA polymerase II small nuclear ribonucleoprotein splicing factor U2AF U2AF2 protein, human carboxy terminal sequence chromatin chromatin structure exon genetic model genetic transcription human in vivo study intron kinetics length molecular evolution priority journal Review RNA binding RNA splicing RNA synthesis spliceosome exon genetics intron RNA splicing Chromatin Exons Humans Introns Ribonucleoprotein, U1 Small Nuclear Ribonucleoproteins, Small Nuclear RNA Polymerase II RNA Splicing Spliceosomes Splicing Factor U2AF
spellingShingle	RNA polymerase II small nuclear ribonucleoprotein RNA polymerase II small nuclear ribonucleoprotein splicing factor U2AF U2AF2 protein, human carboxy terminal sequence chromatin chromatin structure exon genetic model genetic transcription human in vivo study intron kinetics length molecular evolution priority journal Review RNA binding RNA splicing RNA synthesis spliceosome exon genetics intron RNA splicing Chromatin Exons Humans Introns Ribonucleoprotein, U1 Small Nuclear Ribonucleoproteins, Small Nuclear RNA Polymerase II RNA Splicing Spliceosomes Splicing Factor U2AF Kornblihtt, Alberto Rodolfo How Are Short Exons Flanked by Long Introns Defined and Committed to Splicing?
topic_facet	RNA polymerase II small nuclear ribonucleoprotein RNA polymerase II small nuclear ribonucleoprotein splicing factor U2AF U2AF2 protein, human carboxy terminal sequence chromatin chromatin structure exon genetic model genetic transcription human in vivo study intron kinetics length molecular evolution priority journal Review RNA binding RNA splicing RNA synthesis spliceosome exon genetics intron RNA splicing Chromatin Exons Humans Introns Ribonucleoprotein, U1 Small Nuclear Ribonucleoproteins, Small Nuclear RNA Polymerase II RNA Splicing Spliceosomes Splicing Factor U2AF
description	The splice sites (SSs) delimiting an intron are brought together in the earliest step of spliceosome assembly yet it remains obscure how SS pairing occurs, especially when introns are thousands of nucleotides long. Splicing occurs in vivo in mammals within minutes regardless of intron length, implying that SS pairing can instantly follow transcription. Also, factors required for SS pairing, such as the U1 small nuclear ribonucleoprotein (snRNP) and U2AF65, associate with RNA polymerase II (RNAPII), while nucleosomes preferentially bind exonic sequences and associate with U2 snRNP. Based on recent publications, we assume that the 5′ SS-bound U1 snRNP can remain tethered to RNAPII until complete synthesis of the downstream intron and exon. An additional U1 snRNP then binds the downstream 5′ SS, whereas the RNAPII-associated U2AF65 binds the upstream 3′ SS to facilitate SS pairing along with exon definition. Next, the nucleosome-associated U2 snRNP binds the branch site to advance splicing complex assembly. This may explain how RNAPII and chromatin are involved in spliceosome assembly and how introns lengthened during evolution with a relatively minimal compromise in splicing. © 2016 Elsevier Ltd
author	Kornblihtt, Alberto Rodolfo
author_facet	Kornblihtt, Alberto Rodolfo
author_sort	Kornblihtt, Alberto Rodolfo
title	How Are Short Exons Flanked by Long Introns Defined and Committed to Splicing?
title_short	How Are Short Exons Flanked by Long Introns Defined and Committed to Splicing?
title_full	How Are Short Exons Flanked by Long Introns Defined and Committed to Splicing?
title_fullStr	How Are Short Exons Flanked by Long Introns Defined and Committed to Splicing?
title_full_unstemmed	How Are Short Exons Flanked by Long Introns Defined and Committed to Splicing?
title_sort	how are short exons flanked by long introns defined and committed to splicing?
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689525_v32_n10_p596_Hollander http://hdl.handle.net/20.500.12110/paper_01689525_v32_n10_p596_Hollander
work_keys_str_mv	AT kornblihttalbertorodolfo howareshortexonsflankedbylongintronsdefinedandcommittedtosplicing
_version_	1768543654145687552

How Are Short Exons Flanked by Long Introns Defined and Committed to Splicing?

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