The control of root growth by reactive oxygen species in Salix nigra Marsh. seedlings
The production of reactive oxygen species (ROS) in specific regions of Salix seedlings roots seems essential for the normal growth of this organ. We examined the role of different ROS in the control of root development in Salix nigra seedlings, and explored possible mechanisms involved in the regula...
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2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689452_v183_n_p197_Causin http://hdl.handle.net/20.500.12110/paper_01689452_v183_n_p197_Causin |
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paper:paper_01689452_v183_n_p197_Causin2023-06-08T15:18:08Z The control of root growth by reactive oxygen species in Salix nigra Marsh. seedlings Causin, Humberto Fabio Roqueiro, Gonzalo Petrillo, Ezequiel Lainez, Verónica Renee Hydrogen peroxide NADPH-oxidase Oxidative metabolism Peroxidase Superoxide anions Willow hydrogen peroxide peroxidase reactive oxygen metabolite reduced nicotinamide adenine dinucleotide phosphate oxidase superoxide vegetable protein article growth, development and aging metabolism plant root polyacrylamide gel electrophoresis seedling Western blotting willow Blotting, Western Electrophoresis, Polyacrylamide Gel Hydrogen Peroxide NADPH Oxidase Peroxidase Plant Proteins Plant Roots Reactive Oxygen Species Salix Seedling Superoxides Salix Salix nigra The production of reactive oxygen species (ROS) in specific regions of Salix seedlings roots seems essential for the normal growth of this organ. We examined the role of different ROS in the control of root development in Salix nigra seedlings, and explored possible mechanisms involved in the regulation of ROS generation and action. Root growth was not significantly affected by OH quenchers, while it was either partially or completely inhibited in the presence of H 2O 2 or O 2 - scavengers, respectively. O 2 - production was elevated in the root apex, particularly in the subapical meristem and protodermal zones. Apical O 2 - generation activity was correlated to a high level of either Cu/Zn superoxide dismutase protein as well as carbonylated proteins. While NADPH-oxidase (NOX) was probably the main source of O 2 - generation, the existence of other sources should not be discarded. O 2 - production was also high in root hairs during budding, but it markedly decreased when the hair began to actively elongate. Root hair formation increased in the presence of H 2O 2 scavengers, and was suppressed when H 2O 2 or peroxidase inhibitors were supplied. The negative effect of H 2O 2 was partially counteracted by a MAPKK inhibitor. Possible mechanisms of action of the different ROS in comparison with other plant model systems are discussed. © 2011 Elsevier Ireland Ltd. Fil:Causin, H.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Roqueiro, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Petrillo, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Láinez, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689452_v183_n_p197_Causin http://hdl.handle.net/20.500.12110/paper_01689452_v183_n_p197_Causin |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Hydrogen peroxide NADPH-oxidase Oxidative metabolism Peroxidase Superoxide anions Willow hydrogen peroxide peroxidase reactive oxygen metabolite reduced nicotinamide adenine dinucleotide phosphate oxidase superoxide vegetable protein article growth, development and aging metabolism plant root polyacrylamide gel electrophoresis seedling Western blotting willow Blotting, Western Electrophoresis, Polyacrylamide Gel Hydrogen Peroxide NADPH Oxidase Peroxidase Plant Proteins Plant Roots Reactive Oxygen Species Salix Seedling Superoxides Salix Salix nigra |
| spellingShingle |
Hydrogen peroxide NADPH-oxidase Oxidative metabolism Peroxidase Superoxide anions Willow hydrogen peroxide peroxidase reactive oxygen metabolite reduced nicotinamide adenine dinucleotide phosphate oxidase superoxide vegetable protein article growth, development and aging metabolism plant root polyacrylamide gel electrophoresis seedling Western blotting willow Blotting, Western Electrophoresis, Polyacrylamide Gel Hydrogen Peroxide NADPH Oxidase Peroxidase Plant Proteins Plant Roots Reactive Oxygen Species Salix Seedling Superoxides Salix Salix nigra Causin, Humberto Fabio Roqueiro, Gonzalo Petrillo, Ezequiel Lainez, Verónica Renee The control of root growth by reactive oxygen species in Salix nigra Marsh. seedlings |
| topic_facet |
Hydrogen peroxide NADPH-oxidase Oxidative metabolism Peroxidase Superoxide anions Willow hydrogen peroxide peroxidase reactive oxygen metabolite reduced nicotinamide adenine dinucleotide phosphate oxidase superoxide vegetable protein article growth, development and aging metabolism plant root polyacrylamide gel electrophoresis seedling Western blotting willow Blotting, Western Electrophoresis, Polyacrylamide Gel Hydrogen Peroxide NADPH Oxidase Peroxidase Plant Proteins Plant Roots Reactive Oxygen Species Salix Seedling Superoxides Salix Salix nigra |
| description |
The production of reactive oxygen species (ROS) in specific regions of Salix seedlings roots seems essential for the normal growth of this organ. We examined the role of different ROS in the control of root development in Salix nigra seedlings, and explored possible mechanisms involved in the regulation of ROS generation and action. Root growth was not significantly affected by OH quenchers, while it was either partially or completely inhibited in the presence of H 2O 2 or O 2 - scavengers, respectively. O 2 - production was elevated in the root apex, particularly in the subapical meristem and protodermal zones. Apical O 2 - generation activity was correlated to a high level of either Cu/Zn superoxide dismutase protein as well as carbonylated proteins. While NADPH-oxidase (NOX) was probably the main source of O 2 - generation, the existence of other sources should not be discarded. O 2 - production was also high in root hairs during budding, but it markedly decreased when the hair began to actively elongate. Root hair formation increased in the presence of H 2O 2 scavengers, and was suppressed when H 2O 2 or peroxidase inhibitors were supplied. The negative effect of H 2O 2 was partially counteracted by a MAPKK inhibitor. Possible mechanisms of action of the different ROS in comparison with other plant model systems are discussed. © 2011 Elsevier Ireland Ltd. |
| author |
Causin, Humberto Fabio Roqueiro, Gonzalo Petrillo, Ezequiel Lainez, Verónica Renee |
| author_facet |
Causin, Humberto Fabio Roqueiro, Gonzalo Petrillo, Ezequiel Lainez, Verónica Renee |
| author_sort |
Causin, Humberto Fabio |
| title |
The control of root growth by reactive oxygen species in Salix nigra Marsh. seedlings |
| title_short |
The control of root growth by reactive oxygen species in Salix nigra Marsh. seedlings |
| title_full |
The control of root growth by reactive oxygen species in Salix nigra Marsh. seedlings |
| title_fullStr |
The control of root growth by reactive oxygen species in Salix nigra Marsh. seedlings |
| title_full_unstemmed |
The control of root growth by reactive oxygen species in Salix nigra Marsh. seedlings |
| title_sort |
control of root growth by reactive oxygen species in salix nigra marsh. seedlings |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689452_v183_n_p197_Causin http://hdl.handle.net/20.500.12110/paper_01689452_v183_n_p197_Causin |
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