Vero cells persistently infected with tacaribe virus: role of interfering particles in the establishment of the infection

Eight Vero cell sublines (Vero T) persistently infected with wild type Tacaribe virus replicated in different hosts were established. In order to unravel the mecha- nism involved in the initiation and maintenance of persistence, the properties of virus shed by the sublines and the presence of interf...

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Autores principales:	D'Aiutolo, Ana Cristina, Coto, Celia Esther
Publicado:	1986
Materias:	interfering particles persistent infection Tacaribe virus Vero cells cell culture heredity in vitro study monkey nonhuman priority journal tacaribe arenavirus temperature sensitive mutant virus interference virus isolation Animal Animals, Suckling Arenaviridae Arenaviruses, New World Mice Support, Non-U.S. Gov't Vero Cells Viral Interference Virus Replication
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v6_n3_p235_DAiutolo http://hdl.handle.net/20.500.12110/paper_01681702_v6_n3_p235_DAiutolo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01681702_v6_n3_p235_DAiutolo
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spelling	paper:paper_01681702_v6_n3_p235_DAiutolo2023-06-08T15:17:41Z Vero cells persistently infected with tacaribe virus: role of interfering particles in the establishment of the infection D'Aiutolo, Ana Cristina Coto, Celia Esther interfering particles persistent infection Tacaribe virus Vero cells cell culture heredity in vitro study monkey nonhuman persistent infection priority journal tacaribe arenavirus temperature sensitive mutant virus interference virus isolation Animal Animals, Suckling Arenaviridae Arenaviruses, New World Mice Support, Non-U.S. Gov't Vero Cells Viral Interference Virus Replication Eight Vero cell sublines (Vero T) persistently infected with wild type Tacaribe virus replicated in different hosts were established. In order to unravel the mecha- nism involved in the initiation and maintenance of persistence, the properties of virus shed by the sublines and the presence of interfering particles (IP) were analyzed. During the course of infection, persistent virus (Tac-pi) underwent muta- tions although no consistent pattern of virus evolution was observed, ts mutants were isolated from two Vero T sublines, whereas a slow growth variant was shed by another. The remaining sublines released virus resembling wt parental virus. Except for Vero T1 sublines, Vero T cultures shed no detectable IP. These results emphasize the point that neither the emergence of virus mutants nor the synthesis of IP is essential for the maintenance of the persistent state. To define the role of IP in the initiation of persistence, coinfection experiments with a characterized inoculum were performed. For that purpose, attempts were made to obtain IP stocks free from pfu by serial transfers of undiluted virus. Neither enrichment nor amplification of IP occurred, and virus stocks were freed of infectious virus by UV irradiation. If normal Vero cells were infected with Tac-pi virus released by Vero T2, Vero T3, Vero T4, Vero T5, Vero T6, Vero T7 and Vero T10 sublines, a complete destruction of the monolayer without cell recovery was ob- served. In contrast, parental and Vero T1 viruses always originated persistently infected sublines. Similarly, the addition of IP to virus inocula constituted by Tac-pi viruses released by Vero T2, Vero T3, Vero T4, Vero T5, Vero T6, Vero T7 and Vero T10 sublines gave rise to persistently infected cultures. These results suggest that although IP are not important by themselves in the maintenance of persistence, they play a major role in initiation. © 1986. Fil:D'Aiutolo, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1986 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v6_n3_p235_DAiutolo http://hdl.handle.net/20.500.12110/paper_01681702_v6_n3_p235_DAiutolo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	interfering particles persistent infection Tacaribe virus Vero cells cell culture heredity in vitro study monkey nonhuman persistent infection priority journal tacaribe arenavirus temperature sensitive mutant virus interference virus isolation Animal Animals, Suckling Arenaviridae Arenaviruses, New World Mice Support, Non-U.S. Gov't Vero Cells Viral Interference Virus Replication
spellingShingle	interfering particles persistent infection Tacaribe virus Vero cells cell culture heredity in vitro study monkey nonhuman persistent infection priority journal tacaribe arenavirus temperature sensitive mutant virus interference virus isolation Animal Animals, Suckling Arenaviridae Arenaviruses, New World Mice Support, Non-U.S. Gov't Vero Cells Viral Interference Virus Replication D'Aiutolo, Ana Cristina Coto, Celia Esther Vero cells persistently infected with tacaribe virus: role of interfering particles in the establishment of the infection
topic_facet	interfering particles persistent infection Tacaribe virus Vero cells cell culture heredity in vitro study monkey nonhuman persistent infection priority journal tacaribe arenavirus temperature sensitive mutant virus interference virus isolation Animal Animals, Suckling Arenaviridae Arenaviruses, New World Mice Support, Non-U.S. Gov't Vero Cells Viral Interference Virus Replication
description	Eight Vero cell sublines (Vero T) persistently infected with wild type Tacaribe virus replicated in different hosts were established. In order to unravel the mecha- nism involved in the initiation and maintenance of persistence, the properties of virus shed by the sublines and the presence of interfering particles (IP) were analyzed. During the course of infection, persistent virus (Tac-pi) underwent muta- tions although no consistent pattern of virus evolution was observed, ts mutants were isolated from two Vero T sublines, whereas a slow growth variant was shed by another. The remaining sublines released virus resembling wt parental virus. Except for Vero T1 sublines, Vero T cultures shed no detectable IP. These results emphasize the point that neither the emergence of virus mutants nor the synthesis of IP is essential for the maintenance of the persistent state. To define the role of IP in the initiation of persistence, coinfection experiments with a characterized inoculum were performed. For that purpose, attempts were made to obtain IP stocks free from pfu by serial transfers of undiluted virus. Neither enrichment nor amplification of IP occurred, and virus stocks were freed of infectious virus by UV irradiation. If normal Vero cells were infected with Tac-pi virus released by Vero T2, Vero T3, Vero T4, Vero T5, Vero T6, Vero T7 and Vero T10 sublines, a complete destruction of the monolayer without cell recovery was ob- served. In contrast, parental and Vero T1 viruses always originated persistently infected sublines. Similarly, the addition of IP to virus inocula constituted by Tac-pi viruses released by Vero T2, Vero T3, Vero T4, Vero T5, Vero T6, Vero T7 and Vero T10 sublines gave rise to persistently infected cultures. These results suggest that although IP are not important by themselves in the maintenance of persistence, they play a major role in initiation. © 1986.
author	D'Aiutolo, Ana Cristina Coto, Celia Esther
author_facet	D'Aiutolo, Ana Cristina Coto, Celia Esther
author_sort	D'Aiutolo, Ana Cristina
title	Vero cells persistently infected with tacaribe virus: role of interfering particles in the establishment of the infection
title_short	Vero cells persistently infected with tacaribe virus: role of interfering particles in the establishment of the infection
title_full	Vero cells persistently infected with tacaribe virus: role of interfering particles in the establishment of the infection
title_fullStr	Vero cells persistently infected with tacaribe virus: role of interfering particles in the establishment of the infection
title_full_unstemmed	Vero cells persistently infected with tacaribe virus: role of interfering particles in the establishment of the infection
title_sort	vero cells persistently infected with tacaribe virus: role of interfering particles in the establishment of the infection
publishDate	1986
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v6_n3_p235_DAiutolo http://hdl.handle.net/20.500.12110/paper_01681702_v6_n3_p235_DAiutolo
work_keys_str_mv	AT daiutoloanacristina verocellspersistentlyinfectedwithtacaribevirusroleofinterferingparticlesintheestablishmentoftheinfection AT cotoceliaesther verocellspersistentlyinfectedwithtacaribevirusroleofinterferingparticlesintheestablishmentoftheinfection
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Vero cells persistently infected with tacaribe virus: role of interfering particles in the establishment of the infection

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