Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells
The aim of the present study was to analyze the influence of virus origin, mammalian or mosquito cell-derived, on antiviral susceptibility of DENV-2 to entry inhibitors and the association of this effect with any alteration in the mode of entry into the cell. To this end, ten serial passages of DENV...
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2014
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v184_n_p39_Acosta http://hdl.handle.net/20.500.12110/paper_01681702_v184_n_p39_Acosta |
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paper:paper_01681702_v184_n_p39_Acosta2023-06-08T15:17:40Z Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells Acosta, Eliana Gisela Talarico, Laura Beatriz Castilla, Viviana Damonte, Elsa Beatriz Antiviral activity Carrageenan Clathrin-mediated endocytosis Dengue virus Entry inhibitor Sulfated polysaccharide carrageenan chlorpromazine heparin antivirus agent carrageenan heparin virus RNA antiviral activity antiviral susceptibility article controlled study Dengue virus 1 Dengue virus 2 endocytosis host cell in vitro study nonhuman priority journal Vero cell virus entry adaptation animal antiviral resistance cell line Chlorocebus aethiops Dengue virus DNA sequence drug effects genetics growth, development and aging molecular genetics mosquito nucleotide sequence viral plaque assay virus culture Adaptation, Biological Animals Antiviral Agents Carrageenan Cell Line Cercopithecus aethiops Culicidae Dengue Virus DNA Mutational Analysis Drug Resistance, Viral Endocytosis Heparin Molecular Sequence Data RNA, Viral Sequence Analysis, DNA Serial Passage Viral Plaque Assay Virus Internalization The aim of the present study was to analyze the influence of virus origin, mammalian or mosquito cell-derived, on antiviral susceptibility of DENV-2 to entry inhibitors and the association of this effect with any alteration in the mode of entry into the cell. To this end, ten serial passages of DENV-2 were performed in mosquito C6/36 cells or monkey Vero cells and the antiviral susceptibility of each virus passage to sulfated polysaccharides (SPs), like heparin and carrageenans, was evaluated by a virus plaque reduction assay. After serial passaging in Vero cells, DENV-2 became increasingly resistant to SP inhibition whereas the antiviral susceptibility was not altered in virus propagated in C6/36 cells. The change in antiviral susceptibility was associated to a differential mode of entry into the host cell. The route of endocytic entry for productive Vero cell infection was altered from a non-classical clathrin independent pathway for C6/36-grown virus to a clathrin-mediated endocytosis when the virus was serially propagated in Vero cells. Our results show the impact of the cellular system used for successive propagation of DENV on the initial interaction between the host cell and the virion in the next round of infection and the relevant consequences it might have during the in vitro evaluation of entry inhibitors. © 2014 Elsevier B.V. Fil:Acosta, E.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Talarico, L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v184_n_p39_Acosta http://hdl.handle.net/20.500.12110/paper_01681702_v184_n_p39_Acosta |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antiviral activity Carrageenan Clathrin-mediated endocytosis Dengue virus Entry inhibitor Sulfated polysaccharide carrageenan chlorpromazine heparin antivirus agent carrageenan heparin virus RNA antiviral activity antiviral susceptibility article controlled study Dengue virus 1 Dengue virus 2 endocytosis host cell in vitro study nonhuman priority journal Vero cell virus entry adaptation animal antiviral resistance cell line Chlorocebus aethiops Dengue virus DNA sequence drug effects genetics growth, development and aging molecular genetics mosquito nucleotide sequence viral plaque assay virus culture Adaptation, Biological Animals Antiviral Agents Carrageenan Cell Line Cercopithecus aethiops Culicidae Dengue Virus DNA Mutational Analysis Drug Resistance, Viral Endocytosis Heparin Molecular Sequence Data RNA, Viral Sequence Analysis, DNA Serial Passage Viral Plaque Assay Virus Internalization |
| spellingShingle |
Antiviral activity Carrageenan Clathrin-mediated endocytosis Dengue virus Entry inhibitor Sulfated polysaccharide carrageenan chlorpromazine heparin antivirus agent carrageenan heparin virus RNA antiviral activity antiviral susceptibility article controlled study Dengue virus 1 Dengue virus 2 endocytosis host cell in vitro study nonhuman priority journal Vero cell virus entry adaptation animal antiviral resistance cell line Chlorocebus aethiops Dengue virus DNA sequence drug effects genetics growth, development and aging molecular genetics mosquito nucleotide sequence viral plaque assay virus culture Adaptation, Biological Animals Antiviral Agents Carrageenan Cell Line Cercopithecus aethiops Culicidae Dengue Virus DNA Mutational Analysis Drug Resistance, Viral Endocytosis Heparin Molecular Sequence Data RNA, Viral Sequence Analysis, DNA Serial Passage Viral Plaque Assay Virus Internalization Acosta, Eliana Gisela Talarico, Laura Beatriz Castilla, Viviana Damonte, Elsa Beatriz Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells |
| topic_facet |
Antiviral activity Carrageenan Clathrin-mediated endocytosis Dengue virus Entry inhibitor Sulfated polysaccharide carrageenan chlorpromazine heparin antivirus agent carrageenan heparin virus RNA antiviral activity antiviral susceptibility article controlled study Dengue virus 1 Dengue virus 2 endocytosis host cell in vitro study nonhuman priority journal Vero cell virus entry adaptation animal antiviral resistance cell line Chlorocebus aethiops Dengue virus DNA sequence drug effects genetics growth, development and aging molecular genetics mosquito nucleotide sequence viral plaque assay virus culture Adaptation, Biological Animals Antiviral Agents Carrageenan Cell Line Cercopithecus aethiops Culicidae Dengue Virus DNA Mutational Analysis Drug Resistance, Viral Endocytosis Heparin Molecular Sequence Data RNA, Viral Sequence Analysis, DNA Serial Passage Viral Plaque Assay Virus Internalization |
| description |
The aim of the present study was to analyze the influence of virus origin, mammalian or mosquito cell-derived, on antiviral susceptibility of DENV-2 to entry inhibitors and the association of this effect with any alteration in the mode of entry into the cell. To this end, ten serial passages of DENV-2 were performed in mosquito C6/36 cells or monkey Vero cells and the antiviral susceptibility of each virus passage to sulfated polysaccharides (SPs), like heparin and carrageenans, was evaluated by a virus plaque reduction assay. After serial passaging in Vero cells, DENV-2 became increasingly resistant to SP inhibition whereas the antiviral susceptibility was not altered in virus propagated in C6/36 cells. The change in antiviral susceptibility was associated to a differential mode of entry into the host cell. The route of endocytic entry for productive Vero cell infection was altered from a non-classical clathrin independent pathway for C6/36-grown virus to a clathrin-mediated endocytosis when the virus was serially propagated in Vero cells. Our results show the impact of the cellular system used for successive propagation of DENV on the initial interaction between the host cell and the virion in the next round of infection and the relevant consequences it might have during the in vitro evaluation of entry inhibitors. © 2014 Elsevier B.V. |
| author |
Acosta, Eliana Gisela Talarico, Laura Beatriz Castilla, Viviana Damonte, Elsa Beatriz |
| author_facet |
Acosta, Eliana Gisela Talarico, Laura Beatriz Castilla, Viviana Damonte, Elsa Beatriz |
| author_sort |
Acosta, Eliana Gisela |
| title |
Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells |
| title_short |
Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells |
| title_full |
Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells |
| title_fullStr |
Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells |
| title_full_unstemmed |
Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells |
| title_sort |
changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in vero or c6/36 cells |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v184_n_p39_Acosta http://hdl.handle.net/20.500.12110/paper_01681702_v184_n_p39_Acosta |
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| _version_ |
1768543464416346112 |