The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase

The present work was carried out with the aim to analyze the secretome of Trametes versicolor BAFC 2234 grown on tomato juice medium supplemented with copper and manganese. T. versicolor BAFC 2234 was selected among diverse wood dwelling agaricomycetes from Argentina by its ability to cause a strong...

Descripción completa

Guardado en:

Detalles Bibliográficos
Publicado:	2013
Materias:	Laccase Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Laccases Biodegradation Fruits Hardwoods Lignin Proteins Purification Enzymes copper fungal protein hydrolase laccase lignocellulose manganese manganese peroxidase monophenol monooxygenase peroxidase phenol derivative secretome unclassified drug Argentina article carbon source culture medium degradation elution enzyme purification enzyme synthesis extracellular space fruit juice isoelectric point molecular weight nonhuman pH polyacrylamide gel electrophoresis priority journal protein analysis protein secretion tomato Lignins Culture Media Hydrolases Lycopersicon esculentum Monophenol Monooxygenase Oxidoreductases Peroxidase Trametes
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v168_n1_p15_Carabajal http://hdl.handle.net/20.500.12110/paper_01681656_v168_n1_p15_Carabajal
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01681656_v168_n1_p15_Carabajal
record_format	dspace
spelling	paper:paper_01681656_v168_n1_p15_Carabajal2023-06-08T15:17:33Z The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase Laccase Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Laccases Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Biodegradation Fruits Hardwoods Lignin Proteins Purification Enzymes copper fungal protein hydrolase laccase lignocellulose manganese manganese peroxidase monophenol monooxygenase peroxidase phenol derivative secretome unclassified drug Argentina article carbon source culture medium degradation elution enzyme purification enzyme synthesis extracellular space fruit juice isoelectric point molecular weight nonhuman pH polyacrylamide gel electrophoresis priority journal protein analysis protein secretion tomato Trametes versicolor Biodegradation Enzymes Fruits Hardwoods Laccase Lignins Proteins Purification Laccase Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Culture Media Hydrolases Lycopersicon esculentum Monophenol Monooxygenase Oxidoreductases Peroxidase Trametes The present work was carried out with the aim to analyze the secretome of Trametes versicolor BAFC 2234 grown on tomato juice medium supplemented with copper and manganese. T. versicolor BAFC 2234 was selected among diverse wood dwelling agaricomycetes from Argentina by its ability to cause a strong white rot on hardwood and in addition to show high tolerance toward phenolic compounds. A considerable number of the identified proteins were related to the degradation/modification of lignocelluloses. Hydrolases, peroxidases and phenoloxidases were the most abundant enzymes produced under the above-mentioned culture conditions. The lignin-modifying oxidoreductases laccase, manganese peroxidase (MnP) and versatile peroxidase (VP) were successfully purified - the latter for the first time from T. versicolor. The native VP protein has a molecular mass of 45. kDa and an isoelectric point of pH 3.7. The study clearly shows that complex plant-based media being rich in phenolics, such as tomato juice, can stimulate the secretion of a broad set of extracellular lignocellulolytic enzymes. Using such natural products as fungal culture media may give the opportunity to investigate plant biomass decomposition as well as the biodegradation of organic pollutants in an environment close to nature. © 2013 Elsevier B.V. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v168_n1_p15_Carabajal http://hdl.handle.net/20.500.12110/paper_01681656_v168_n1_p15_Carabajal
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Laccase Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Laccases Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Biodegradation Fruits Hardwoods Lignin Proteins Purification Enzymes copper fungal protein hydrolase laccase lignocellulose manganese manganese peroxidase monophenol monooxygenase peroxidase phenol derivative secretome unclassified drug Argentina article carbon source culture medium degradation elution enzyme purification enzyme synthesis extracellular space fruit juice isoelectric point molecular weight nonhuman pH polyacrylamide gel electrophoresis priority journal protein analysis protein secretion tomato Trametes versicolor Biodegradation Enzymes Fruits Hardwoods Laccase Lignins Proteins Purification Laccase Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Culture Media Hydrolases Lycopersicon esculentum Monophenol Monooxygenase Oxidoreductases Peroxidase Trametes
spellingShingle	Laccase Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Laccases Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Biodegradation Fruits Hardwoods Lignin Proteins Purification Enzymes copper fungal protein hydrolase laccase lignocellulose manganese manganese peroxidase monophenol monooxygenase peroxidase phenol derivative secretome unclassified drug Argentina article carbon source culture medium degradation elution enzyme purification enzyme synthesis extracellular space fruit juice isoelectric point molecular weight nonhuman pH polyacrylamide gel electrophoresis priority journal protein analysis protein secretion tomato Trametes versicolor Biodegradation Enzymes Fruits Hardwoods Laccase Lignins Proteins Purification Laccase Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Culture Media Hydrolases Lycopersicon esculentum Monophenol Monooxygenase Oxidoreductases Peroxidase Trametes The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase
topic_facet	Laccase Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Laccases Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Biodegradation Fruits Hardwoods Lignin Proteins Purification Enzymes copper fungal protein hydrolase laccase lignocellulose manganese manganese peroxidase monophenol monooxygenase peroxidase phenol derivative secretome unclassified drug Argentina article carbon source culture medium degradation elution enzyme purification enzyme synthesis extracellular space fruit juice isoelectric point molecular weight nonhuman pH polyacrylamide gel electrophoresis priority journal protein analysis protein secretion tomato Trametes versicolor Biodegradation Enzymes Fruits Hardwoods Laccase Lignins Proteins Purification Laccase Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Culture Media Hydrolases Lycopersicon esculentum Monophenol Monooxygenase Oxidoreductases Peroxidase Trametes
description	The present work was carried out with the aim to analyze the secretome of Trametes versicolor BAFC 2234 grown on tomato juice medium supplemented with copper and manganese. T. versicolor BAFC 2234 was selected among diverse wood dwelling agaricomycetes from Argentina by its ability to cause a strong white rot on hardwood and in addition to show high tolerance toward phenolic compounds. A considerable number of the identified proteins were related to the degradation/modification of lignocelluloses. Hydrolases, peroxidases and phenoloxidases were the most abundant enzymes produced under the above-mentioned culture conditions. The lignin-modifying oxidoreductases laccase, manganese peroxidase (MnP) and versatile peroxidase (VP) were successfully purified - the latter for the first time from T. versicolor. The native VP protein has a molecular mass of 45. kDa and an isoelectric point of pH 3.7. The study clearly shows that complex plant-based media being rich in phenolics, such as tomato juice, can stimulate the secretion of a broad set of extracellular lignocellulolytic enzymes. Using such natural products as fungal culture media may give the opportunity to investigate plant biomass decomposition as well as the biodegradation of organic pollutants in an environment close to nature. © 2013 Elsevier B.V.
title	The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase
title_short	The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase
title_full	The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase
title_fullStr	The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase
title_full_unstemmed	The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase
title_sort	secretome of trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase
publishDate	2013
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v168_n1_p15_Carabajal http://hdl.handle.net/20.500.12110/paper_01681656_v168_n1_p15_Carabajal
_version_	1768544361833824256

The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase

Ejemplares similares