Plant cell proliferation inside an inorganic host
In recent years, much attention has been paid to plant cell culture as a tool for the production of secondary metabolites and the expression of recombinant proteins. Plant cell immobilization offers many advantages for biotechnological processes. However, the most extended matrices employed, such as...
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2007
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v127_n3_p542_Perullini http://hdl.handle.net/20.500.12110/paper_01681656_v127_n3_p542_Perullini |
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paper:paper_01681656_v127_n3_p542_Perullini2023-06-08T15:17:32Z Plant cell proliferation inside an inorganic host Perullini, Ana Mercedes Rivero Pérez, María Mercedes Jobbagy, Matias Mentaberry, Alejandro Néstor Aldabe Bilmes, Sara Alfonsina Dora Plant cell immobilization Silica matrix Sol-gel Bacteria Plant cell culture Plants (botany) Silica Sol-gels Biological contamination Plant cell immobilization Silica matrix Sol-gel chemistry Biotechnology analytic method article cell growth cell proliferation contamination extracellular matrix host immobilized cell microbial contamination nonhuman plant cell priority journal Cell Culture Techniques Cell Proliferation Cells, Immobilized Phloem Silicon Dioxide Tobacco In recent years, much attention has been paid to plant cell culture as a tool for the production of secondary metabolites and the expression of recombinant proteins. Plant cell immobilization offers many advantages for biotechnological processes. However, the most extended matrices employed, such as calcium-alginate, cannot fully protect entrapped cells. Sol-gel chemistry of silicates has emerged as an outstanding strategy to obtain biomaterials in which living cells are truly protected. This field of research is rapidly developing and a large number of bacteria and yeast-entrapping ceramics have already been designed for different applications. But even mild thermal and chemical conditions employed in sol-gel synthesis may result harmful to cells of higher organisms. Here we present a method for the immobilization of plant cells that allows cell growth at cavities created inside a silica matrix. Plant cell proliferation was monitored for a 6-month period, at the end of which plant calli of more than 1 mm in diameter were observed inside the inorganic host. The resulting hybrid device had good mechanical stability and proved to be an effective barrier against biological contamination, suggesting that it could be employed for long-term plant cell entrapment applications. © 2006 Elsevier B.V. All rights reserved. Fil:Perullini, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rivero, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jobbágy, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mentaberry, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bilmes, S.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v127_n3_p542_Perullini http://hdl.handle.net/20.500.12110/paper_01681656_v127_n3_p542_Perullini |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Plant cell immobilization Silica matrix Sol-gel Bacteria Plant cell culture Plants (botany) Silica Sol-gels Biological contamination Plant cell immobilization Silica matrix Sol-gel chemistry Biotechnology analytic method article cell growth cell proliferation contamination extracellular matrix host immobilized cell microbial contamination nonhuman plant cell priority journal Cell Culture Techniques Cell Proliferation Cells, Immobilized Phloem Silicon Dioxide Tobacco |
| spellingShingle |
Plant cell immobilization Silica matrix Sol-gel Bacteria Plant cell culture Plants (botany) Silica Sol-gels Biological contamination Plant cell immobilization Silica matrix Sol-gel chemistry Biotechnology analytic method article cell growth cell proliferation contamination extracellular matrix host immobilized cell microbial contamination nonhuman plant cell priority journal Cell Culture Techniques Cell Proliferation Cells, Immobilized Phloem Silicon Dioxide Tobacco Perullini, Ana Mercedes Rivero Pérez, María Mercedes Jobbagy, Matias Mentaberry, Alejandro Néstor Aldabe Bilmes, Sara Alfonsina Dora Plant cell proliferation inside an inorganic host |
| topic_facet |
Plant cell immobilization Silica matrix Sol-gel Bacteria Plant cell culture Plants (botany) Silica Sol-gels Biological contamination Plant cell immobilization Silica matrix Sol-gel chemistry Biotechnology analytic method article cell growth cell proliferation contamination extracellular matrix host immobilized cell microbial contamination nonhuman plant cell priority journal Cell Culture Techniques Cell Proliferation Cells, Immobilized Phloem Silicon Dioxide Tobacco |
| description |
In recent years, much attention has been paid to plant cell culture as a tool for the production of secondary metabolites and the expression of recombinant proteins. Plant cell immobilization offers many advantages for biotechnological processes. However, the most extended matrices employed, such as calcium-alginate, cannot fully protect entrapped cells. Sol-gel chemistry of silicates has emerged as an outstanding strategy to obtain biomaterials in which living cells are truly protected. This field of research is rapidly developing and a large number of bacteria and yeast-entrapping ceramics have already been designed for different applications. But even mild thermal and chemical conditions employed in sol-gel synthesis may result harmful to cells of higher organisms. Here we present a method for the immobilization of plant cells that allows cell growth at cavities created inside a silica matrix. Plant cell proliferation was monitored for a 6-month period, at the end of which plant calli of more than 1 mm in diameter were observed inside the inorganic host. The resulting hybrid device had good mechanical stability and proved to be an effective barrier against biological contamination, suggesting that it could be employed for long-term plant cell entrapment applications. © 2006 Elsevier B.V. All rights reserved. |
| author |
Perullini, Ana Mercedes Rivero Pérez, María Mercedes Jobbagy, Matias Mentaberry, Alejandro Néstor Aldabe Bilmes, Sara Alfonsina Dora |
| author_facet |
Perullini, Ana Mercedes Rivero Pérez, María Mercedes Jobbagy, Matias Mentaberry, Alejandro Néstor Aldabe Bilmes, Sara Alfonsina Dora |
| author_sort |
Perullini, Ana Mercedes |
| title |
Plant cell proliferation inside an inorganic host |
| title_short |
Plant cell proliferation inside an inorganic host |
| title_full |
Plant cell proliferation inside an inorganic host |
| title_fullStr |
Plant cell proliferation inside an inorganic host |
| title_full_unstemmed |
Plant cell proliferation inside an inorganic host |
| title_sort |
plant cell proliferation inside an inorganic host |
| publishDate |
2007 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v127_n3_p542_Perullini http://hdl.handle.net/20.500.12110/paper_01681656_v127_n3_p542_Perullini |
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AT perullinianamercedes plantcellproliferationinsideaninorganichost AT riveroperezmariamercedes plantcellproliferationinsideaninorganichost AT jobbagymatias plantcellproliferationinsideaninorganichost AT mentaberryalejandronestor plantcellproliferationinsideaninorganichost AT aldabebilmessaraalfonsinadora plantcellproliferationinsideaninorganichost |
| _version_ |
1768545046355771392 |