High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow

Transgenic farm animals have been proposed as an alternative to current bioreactors for large scale production of biopharmaceuticals. However, the efficiency of both methods in the production of the same protein has not yet been established. Here we report the production of recombinant human growth...

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Autores principales:	Barañao, José Lino S., Fernández, Natalia C., Judewicz, Norberto D.
Publicado:	2006
Materias:	Animal biotechnology Recombinant hGH Transgenic cloned cow Biotechnology Cost effectiveness Dairy products Drug products Escherichia coli Hormones human growth hormone animal cell animal experiment article breed cattle clone cost effectiveness analysis cow fertility fetus hematological parameters hormone synthesis milk nonhuman priority journal progeny transgenic animal Animals Animals, Genetically Modified Cattle Cloning, Organism Human Growth Hormone Humans Milk Proteins Recombinant Proteins Animalia Bos taurus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v124_n2_p469_Salamone http://hdl.handle.net/20.500.12110/paper_01681656_v124_n2_p469_Salamone
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01681656_v124_n2_p469_Salamone
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spelling	paper:paper_01681656_v124_n2_p469_Salamone2023-06-08T15:17:31Z High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow Barañao, José Lino S. Fernández, Natalia C. Judewicz, Norberto D. Animal biotechnology Recombinant hGH Transgenic cloned cow Biotechnology Cost effectiveness Dairy products Drug products Escherichia coli Animal biotechnology Recombinant hGH Transgenic cloned cow Hormones human growth hormone animal cell animal experiment article breed cattle clone cost effectiveness analysis cow Escherichia coli fertility fetus hematological parameters hormone synthesis milk nonhuman priority journal progeny transgenic animal Animals Animals, Genetically Modified Cattle Cloning, Organism Human Growth Hormone Humans Milk Proteins Recombinant Proteins Animalia Bos taurus Transgenic farm animals have been proposed as an alternative to current bioreactors for large scale production of biopharmaceuticals. However, the efficiency of both methods in the production of the same protein has not yet been established. Here we report the production of recombinant human growth hormone (hGH) in the milk of a cloned transgenic cow at levels of up to 5 g l-1. The hormone is identical to that currently produced by expression in E. coli. In addition, the hematological and somatometric parameters of the cloned transgenic cow are within the normal range for the breed and it is fertile and capable of producing normal offspring. These results demonstrate that transgenic cattle can be used as a cost-effective alternative for the production of this hormone. © 2006. Fil:Barañao, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fernández, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Judewicz, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v124_n2_p469_Salamone http://hdl.handle.net/20.500.12110/paper_01681656_v124_n2_p469_Salamone
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Animal biotechnology Recombinant hGH Transgenic cloned cow Biotechnology Cost effectiveness Dairy products Drug products Escherichia coli Animal biotechnology Recombinant hGH Transgenic cloned cow Hormones human growth hormone animal cell animal experiment article breed cattle clone cost effectiveness analysis cow Escherichia coli fertility fetus hematological parameters hormone synthesis milk nonhuman priority journal progeny transgenic animal Animals Animals, Genetically Modified Cattle Cloning, Organism Human Growth Hormone Humans Milk Proteins Recombinant Proteins Animalia Bos taurus
spellingShingle	Animal biotechnology Recombinant hGH Transgenic cloned cow Biotechnology Cost effectiveness Dairy products Drug products Escherichia coli Animal biotechnology Recombinant hGH Transgenic cloned cow Hormones human growth hormone animal cell animal experiment article breed cattle clone cost effectiveness analysis cow Escherichia coli fertility fetus hematological parameters hormone synthesis milk nonhuman priority journal progeny transgenic animal Animals Animals, Genetically Modified Cattle Cloning, Organism Human Growth Hormone Humans Milk Proteins Recombinant Proteins Animalia Bos taurus Barañao, José Lino S. Fernández, Natalia C. Judewicz, Norberto D. High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow
topic_facet	Animal biotechnology Recombinant hGH Transgenic cloned cow Biotechnology Cost effectiveness Dairy products Drug products Escherichia coli Animal biotechnology Recombinant hGH Transgenic cloned cow Hormones human growth hormone animal cell animal experiment article breed cattle clone cost effectiveness analysis cow Escherichia coli fertility fetus hematological parameters hormone synthesis milk nonhuman priority journal progeny transgenic animal Animals Animals, Genetically Modified Cattle Cloning, Organism Human Growth Hormone Humans Milk Proteins Recombinant Proteins Animalia Bos taurus
description	Transgenic farm animals have been proposed as an alternative to current bioreactors for large scale production of biopharmaceuticals. However, the efficiency of both methods in the production of the same protein has not yet been established. Here we report the production of recombinant human growth hormone (hGH) in the milk of a cloned transgenic cow at levels of up to 5 g l-1. The hormone is identical to that currently produced by expression in E. coli. In addition, the hematological and somatometric parameters of the cloned transgenic cow are within the normal range for the breed and it is fertile and capable of producing normal offspring. These results demonstrate that transgenic cattle can be used as a cost-effective alternative for the production of this hormone. © 2006.
author	Barañao, José Lino S. Fernández, Natalia C. Judewicz, Norberto D.
author_facet	Barañao, José Lino S. Fernández, Natalia C. Judewicz, Norberto D.
author_sort	Barañao, José Lino S.
title	High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow
title_short	High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow
title_full	High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow
title_fullStr	High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow
title_full_unstemmed	High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow
title_sort	high level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow
publishDate	2006
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v124_n2_p469_Salamone http://hdl.handle.net/20.500.12110/paper_01681656_v124_n2_p469_Salamone
work_keys_str_mv	AT baranaojoselinos highlevelexpressionofbioactiverecombinanthumangrowthhormoneinthemilkofaclonedtransgeniccow AT fernandeznataliac highlevelexpressionofbioactiverecombinanthumangrowthhormoneinthemilkofaclonedtransgeniccow AT judewicznorbertod highlevelexpressionofbioactiverecombinanthumangrowthhormoneinthemilkofaclonedtransgeniccow
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High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow

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