Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina
Fusarium graminearum is one of the most important pathogens isolated from small cereal grains with Fusarium Head Blight symptoms. The presence of this fungus is often linked to the occurrence of several mycotoxins in barley and wheat. The aim of our study was to characterize trichothecene genotypes...
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paper:paper_01681605_v179_n_p57_Castanares2023-06-08T15:17:20Z Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina Patriarca, Andrea Rosana Chemical production profiles Fusarium graminearum Genotype Hordeum vulgare Trichothecenes 15 acetylvomitoxin 3 acetyldeoxynivalenol nivalenol trichothecene unclassified drug vomitoxin Argentina article barley controlled study Fusarium graminearum gas chromatography genotype mass fragmentography mycotoxicosis nonhuman Chemical production profiles Fusarium graminearum Genotype Hordeum vulgare Trichothecenes Argentina Fusarium Genotype Hordeum Mycotoxins Polymerase Chain Reaction Fusarium graminearum is one of the most important pathogens isolated from small cereal grains with Fusarium Head Blight symptoms. The presence of this fungus is often linked to the occurrence of several mycotoxins in barley and wheat. The aim of our study was to characterize trichothecene genotypes and production profiles of F. graminearum sensu stricto isolates obtained from barley grains in Argentina. A total of 110 F. graminearum s.s. isolates were analyzed by PCR assays to predict deoxynivalenol (DON), 15-acetyldeoxynivalenol (15-ADON), 3-acetyldeoxynivalenol (3-ADON) and nivalenol (NIV) production, and all isolates were found to belong to the same molecular 15-ADON genotype. Trichothecene production in autoclaved rice was analyzed by using gas chromatography (GC) and confirmed by GC-MS. Of the 110 isolates, 95% were able to produce DON, 71% produced 15-ADON, 63% 3-ADON and 52% NIV. With the exception of a single isolate, all isolates that produced NIV, also produced DON. However, the NIV production was very low, ranging from 0.13 to 0.30. μg/g. Six different production profiles of DON and its acetyl-derivatives were detected, the predominant being simultaneous production of DON, 3-ADON and 15-ADON, followed by DON production, and DON and 15-ADON co-production. This work is the first attempt to characterize the trichothecene genotypes and production profiles of F. graminearum s.s. isolates from Argentinean barley. © 2014 Elsevier B.V. Fil:Patriarca, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681605_v179_n_p57_Castanares http://hdl.handle.net/20.500.12110/paper_01681605_v179_n_p57_Castanares |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chemical production profiles Fusarium graminearum Genotype Hordeum vulgare Trichothecenes 15 acetylvomitoxin 3 acetyldeoxynivalenol nivalenol trichothecene unclassified drug vomitoxin Argentina article barley controlled study Fusarium graminearum gas chromatography genotype mass fragmentography mycotoxicosis nonhuman Chemical production profiles Fusarium graminearum Genotype Hordeum vulgare Trichothecenes Argentina Fusarium Genotype Hordeum Mycotoxins Polymerase Chain Reaction |
| spellingShingle |
Chemical production profiles Fusarium graminearum Genotype Hordeum vulgare Trichothecenes 15 acetylvomitoxin 3 acetyldeoxynivalenol nivalenol trichothecene unclassified drug vomitoxin Argentina article barley controlled study Fusarium graminearum gas chromatography genotype mass fragmentography mycotoxicosis nonhuman Chemical production profiles Fusarium graminearum Genotype Hordeum vulgare Trichothecenes Argentina Fusarium Genotype Hordeum Mycotoxins Polymerase Chain Reaction Patriarca, Andrea Rosana Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina |
| topic_facet |
Chemical production profiles Fusarium graminearum Genotype Hordeum vulgare Trichothecenes 15 acetylvomitoxin 3 acetyldeoxynivalenol nivalenol trichothecene unclassified drug vomitoxin Argentina article barley controlled study Fusarium graminearum gas chromatography genotype mass fragmentography mycotoxicosis nonhuman Chemical production profiles Fusarium graminearum Genotype Hordeum vulgare Trichothecenes Argentina Fusarium Genotype Hordeum Mycotoxins Polymerase Chain Reaction |
| description |
Fusarium graminearum is one of the most important pathogens isolated from small cereal grains with Fusarium Head Blight symptoms. The presence of this fungus is often linked to the occurrence of several mycotoxins in barley and wheat. The aim of our study was to characterize trichothecene genotypes and production profiles of F. graminearum sensu stricto isolates obtained from barley grains in Argentina. A total of 110 F. graminearum s.s. isolates were analyzed by PCR assays to predict deoxynivalenol (DON), 15-acetyldeoxynivalenol (15-ADON), 3-acetyldeoxynivalenol (3-ADON) and nivalenol (NIV) production, and all isolates were found to belong to the same molecular 15-ADON genotype. Trichothecene production in autoclaved rice was analyzed by using gas chromatography (GC) and confirmed by GC-MS. Of the 110 isolates, 95% were able to produce DON, 71% produced 15-ADON, 63% 3-ADON and 52% NIV. With the exception of a single isolate, all isolates that produced NIV, also produced DON. However, the NIV production was very low, ranging from 0.13 to 0.30. μg/g. Six different production profiles of DON and its acetyl-derivatives were detected, the predominant being simultaneous production of DON, 3-ADON and 15-ADON, followed by DON production, and DON and 15-ADON co-production. This work is the first attempt to characterize the trichothecene genotypes and production profiles of F. graminearum s.s. isolates from Argentinean barley. © 2014 Elsevier B.V. |
| author |
Patriarca, Andrea Rosana |
| author_facet |
Patriarca, Andrea Rosana |
| author_sort |
Patriarca, Andrea Rosana |
| title |
Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina |
| title_short |
Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina |
| title_full |
Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina |
| title_fullStr |
Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina |
| title_full_unstemmed |
Trichothecene genotypes and production profiles of Fusarium graminearum isolates obtained from barley cultivated in Argentina |
| title_sort |
trichothecene genotypes and production profiles of fusarium graminearum isolates obtained from barley cultivated in argentina |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681605_v179_n_p57_Castanares http://hdl.handle.net/20.500.12110/paper_01681605_v179_n_p57_Castanares |
| work_keys_str_mv |
AT patriarcaandrearosana trichothecenegenotypesandproductionprofilesoffusariumgraminearumisolatesobtainedfrombarleycultivatedinargentina |
| _version_ |
1768542641758142464 |