Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha

The placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochem...

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Autores principales:	Gambino, Yésica Paola, Calvo, Juan Carlos, Varone, Cecilia Laura
Publicado:	2012
Materias:	17β-estradiol Estrogen receptor Gene expression Leptin Placenta estradiol estrogen receptor alpha leptin mitogen activated protein kinase protein kinase B article controlled study enzyme activation hormonal regulation hormone action hormone synthesis human human cell placenta priority journal Cell Membrane Cell Nucleus Estradiol Estrogen Receptor alpha Female Gene Expression Regulation Gene Silencing Humans Models, Biological Pregnancy Protein Binding Protein Transport Serum Albumin, Bovine Signal Transduction
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674889_v1823_n4_p900_Gambino http://hdl.handle.net/20.500.12110/paper_01674889_v1823_n4_p900_Gambino
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01674889_v1823_n4_p900_Gambino
record_format	dspace
spelling	paper:paper_01674889_v1823_n4_p900_Gambino2023-06-08T15:16:28Z Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha Gambino, Yésica Paola Calvo, Juan Carlos Varone, Cecilia Laura 17β-estradiol Estrogen receptor Gene expression Leptin Placenta estradiol estrogen receptor alpha leptin mitogen activated protein kinase protein kinase B article controlled study enzyme activation hormonal regulation hormone action hormone synthesis human human cell placenta priority journal Cell Membrane Cell Nucleus Estradiol Estrogen Receptor alpha Female Gene Expression Regulation Gene Silencing Humans Leptin Models, Biological Placenta Pregnancy Protein Binding Protein Transport Serum Albumin, Bovine Signal Transduction The placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol (E 2) up-regulates placental leptin expression. To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, in the current study we examined the effect of membrane-constrained E 2 conjugate, E-BSA, on leptin expression in human placental cells. We have found that leptin expression was induced by E-BSA both in BeWo cells and human placental explants, suggesting that E 2 also exerts its effects through membrane receptors. Moreover E-BSA rapidly activated different MAPKs and AKT pathways, and these pathways were involved in E 2 induced placental leptin expression. On the other hand we demonstrated the presence of ERα associated to the plasma membrane of BeWo cells. We showed that E 2 genomic and nongenomic actions could be mediated by ERα. Supporting this idea, the downregulation of ERα level through a specific siRNA, decreased E-BSA effects on leptin expression. Taken together, these results provide new evidence of the mechanisms whereby E 2 regulates leptin expression in placenta and support the importance of leptin in placental physiology. © 2012 Elsevier B.V.. Fil:Gambino, Y.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Varone, C.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674889_v1823_n4_p900_Gambino http://hdl.handle.net/20.500.12110/paper_01674889_v1823_n4_p900_Gambino
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	17β-estradiol Estrogen receptor Gene expression Leptin Placenta estradiol estrogen receptor alpha leptin mitogen activated protein kinase protein kinase B article controlled study enzyme activation hormonal regulation hormone action hormone synthesis human human cell placenta priority journal Cell Membrane Cell Nucleus Estradiol Estrogen Receptor alpha Female Gene Expression Regulation Gene Silencing Humans Leptin Models, Biological Placenta Pregnancy Protein Binding Protein Transport Serum Albumin, Bovine Signal Transduction
spellingShingle	17β-estradiol Estrogen receptor Gene expression Leptin Placenta estradiol estrogen receptor alpha leptin mitogen activated protein kinase protein kinase B article controlled study enzyme activation hormonal regulation hormone action hormone synthesis human human cell placenta priority journal Cell Membrane Cell Nucleus Estradiol Estrogen Receptor alpha Female Gene Expression Regulation Gene Silencing Humans Leptin Models, Biological Placenta Pregnancy Protein Binding Protein Transport Serum Albumin, Bovine Signal Transduction Gambino, Yésica Paola Calvo, Juan Carlos Varone, Cecilia Laura Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
topic_facet	17β-estradiol Estrogen receptor Gene expression Leptin Placenta estradiol estrogen receptor alpha leptin mitogen activated protein kinase protein kinase B article controlled study enzyme activation hormonal regulation hormone action hormone synthesis human human cell placenta priority journal Cell Membrane Cell Nucleus Estradiol Estrogen Receptor alpha Female Gene Expression Regulation Gene Silencing Humans Leptin Models, Biological Placenta Pregnancy Protein Binding Protein Transport Serum Albumin, Bovine Signal Transduction
description	The placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol (E 2) up-regulates placental leptin expression. To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, in the current study we examined the effect of membrane-constrained E 2 conjugate, E-BSA, on leptin expression in human placental cells. We have found that leptin expression was induced by E-BSA both in BeWo cells and human placental explants, suggesting that E 2 also exerts its effects through membrane receptors. Moreover E-BSA rapidly activated different MAPKs and AKT pathways, and these pathways were involved in E 2 induced placental leptin expression. On the other hand we demonstrated the presence of ERα associated to the plasma membrane of BeWo cells. We showed that E 2 genomic and nongenomic actions could be mediated by ERα. Supporting this idea, the downregulation of ERα level through a specific siRNA, decreased E-BSA effects on leptin expression. Taken together, these results provide new evidence of the mechanisms whereby E 2 regulates leptin expression in placenta and support the importance of leptin in placental physiology. © 2012 Elsevier B.V..
author	Gambino, Yésica Paola Calvo, Juan Carlos Varone, Cecilia Laura
author_facet	Gambino, Yésica Paola Calvo, Juan Carlos Varone, Cecilia Laura
author_sort	Gambino, Yésica Paola
title	Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
title_short	Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
title_full	Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
title_fullStr	Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
title_full_unstemmed	Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
title_sort	regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
publishDate	2012
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674889_v1823_n4_p900_Gambino http://hdl.handle.net/20.500.12110/paper_01674889_v1823_n4_p900_Gambino
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Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha

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