Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa

Phorbol esters stimulate cyclic adenosine 3′,5′-monophosphate (cAMP) accumulation in hamster spermatozoa under conditions for in vitro capacitation. The 20-50-fold elevation of cAMP levels induced by 1 μM phorbol 12-myristate 13-acetate (PMA) in spermatozoa depends on the presence of sodium bicarbon...

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Detalles Bibliográficos
Autores principales: Muschietti, Jorge P., Flawiá, Mirtha María, Tezón, Jorge G.
Publicado: 1990
Materias:
(Hamster spermatozoa)
Adenylate cyclase
Bicarbonate
cyclic AMP
Phorbol myristate acetate
adenylate cyclase
bicarbonate
cyclic amp
phorbol ester
animal cell
article
cell membrane
hamster
nonhuman
priority journal
spermatozoon
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
Adenylate Cyclase
Animal
Bicarbonates
Biological Transport
Cyclic AMP
Enzyme Activation
Epididymis
Hamsters
Hydrogen-Ion Concentration
In Vitro
Male
Phorbol 12,13-Dibutyrate
Sodium
Sodium Bicarbonate
Sperm Capacitation
Spermatozoa
Support, Non-U.S. Gov't
Animalia
Cricetinae
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674889_v1054_n2_p231_Visconti
http://hdl.handle.net/20.500.12110/paper_01674889_v1054_n2_p231_Visconti
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_01674889_v1054_n2_p231_Visconti
record_format dspace
spelling paper:paper_01674889_v1054_n2_p231_Visconti2023-06-08T15:16:25Z Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa Muschietti, Jorge P. Flawiá, Mirtha María Tezón, Jorge G. (Hamster spermatozoa) Adenylate cyclase Bicarbonate cyclic AMP Phorbol myristate acetate adenylate cyclase bicarbonate cyclic amp phorbol ester animal cell article cell membrane hamster nonhuman priority journal spermatozoon 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid Adenylate Cyclase Animal Bicarbonates Biological Transport Cyclic AMP Enzyme Activation Epididymis Hamsters Hydrogen-Ion Concentration In Vitro Male Phorbol 12,13-Dibutyrate Sodium Sodium Bicarbonate Sperm Capacitation Spermatozoa Support, Non-U.S. Gov't Animalia Cricetinae Phorbol esters stimulate cyclic adenosine 3′,5′-monophosphate (cAMP) accumulation in hamster spermatozoa under conditions for in vitro capacitation. The 20-50-fold elevation of cAMP levels induced by 1 μM phorbol 12-myristate 13-acetate (PMA) in spermatozoa depends on the presence of sodium bicarbonate in the medium (ED50: 15 mM) and it is independent of extracellular pH. Sodium bicarbonate stimulates adenylate cyclase activity in membrane preparations by 4-fold (ED50: 40 mM). After sulubilization, the bicarbonate-sensitive moiety elutes as a single peak of 55 kDa in a gel filtration column. Blockers of bicarbonate chloride antiporters diisothiocyanate stilbene 2,2′-disulfonic acid (DIDS) or acetamido 4′-isothiocyanate stilbene 2,2′-disulfonic acid (SITS) inhibit the bicarbonate dependent PMA effect on cAMP in living spermatozoa (ED50: 100 μM). Maximal (85%) inhibition in cAMP accumulation is observed at 1 mM. Motility is inhibited only at high concentrations of the blockers. Pretreatment of living cells with 1 mM DIDS does not affect membrane adenylate cyclase activity which remains responsive to bicarbonate. These results suggest that controlled transport of bicarbonate through the sperm plasma membrane could be associated to the regulation of cAMP synthesis. © 1990. Fil:Muschietti, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flawia, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tezon, J.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1990 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674889_v1054_n2_p231_Visconti http://hdl.handle.net/20.500.12110/paper_01674889_v1054_n2_p231_Visconti
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic (Hamster spermatozoa)
Adenylate cyclase
Bicarbonate
cyclic AMP
Phorbol myristate acetate
adenylate cyclase
bicarbonate
cyclic amp
phorbol ester
animal cell
article
cell membrane
hamster
nonhuman
priority journal
spermatozoon
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
Adenylate Cyclase
Animal
Bicarbonates
Biological Transport
Cyclic AMP
Enzyme Activation
Epididymis
Hamsters
Hydrogen-Ion Concentration
In Vitro
Male
Phorbol 12,13-Dibutyrate
Sodium
Sodium Bicarbonate
Sperm Capacitation
Spermatozoa
Support, Non-U.S. Gov't
Animalia
Cricetinae
spellingShingle (Hamster spermatozoa)
Adenylate cyclase
Bicarbonate
cyclic AMP
Phorbol myristate acetate
adenylate cyclase
bicarbonate
cyclic amp
phorbol ester
animal cell
article
cell membrane
hamster
nonhuman
priority journal
spermatozoon
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
Adenylate Cyclase
Animal
Bicarbonates
Biological Transport
Cyclic AMP
Enzyme Activation
Epididymis
Hamsters
Hydrogen-Ion Concentration
In Vitro
Male
Phorbol 12,13-Dibutyrate
Sodium
Sodium Bicarbonate
Sperm Capacitation
Spermatozoa
Support, Non-U.S. Gov't
Animalia
Cricetinae
Muschietti, Jorge P.
Flawiá, Mirtha María
Tezón, Jorge G.
Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa
topic_facet (Hamster spermatozoa)
Adenylate cyclase
Bicarbonate
cyclic AMP
Phorbol myristate acetate
adenylate cyclase
bicarbonate
cyclic amp
phorbol ester
animal cell
article
cell membrane
hamster
nonhuman
priority journal
spermatozoon
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
Adenylate Cyclase
Animal
Bicarbonates
Biological Transport
Cyclic AMP
Enzyme Activation
Epididymis
Hamsters
Hydrogen-Ion Concentration
In Vitro
Male
Phorbol 12,13-Dibutyrate
Sodium
Sodium Bicarbonate
Sperm Capacitation
Spermatozoa
Support, Non-U.S. Gov't
Animalia
Cricetinae
description Phorbol esters stimulate cyclic adenosine 3′,5′-monophosphate (cAMP) accumulation in hamster spermatozoa under conditions for in vitro capacitation. The 20-50-fold elevation of cAMP levels induced by 1 μM phorbol 12-myristate 13-acetate (PMA) in spermatozoa depends on the presence of sodium bicarbonate in the medium (ED50: 15 mM) and it is independent of extracellular pH. Sodium bicarbonate stimulates adenylate cyclase activity in membrane preparations by 4-fold (ED50: 40 mM). After sulubilization, the bicarbonate-sensitive moiety elutes as a single peak of 55 kDa in a gel filtration column. Blockers of bicarbonate chloride antiporters diisothiocyanate stilbene 2,2′-disulfonic acid (DIDS) or acetamido 4′-isothiocyanate stilbene 2,2′-disulfonic acid (SITS) inhibit the bicarbonate dependent PMA effect on cAMP in living spermatozoa (ED50: 100 μM). Maximal (85%) inhibition in cAMP accumulation is observed at 1 mM. Motility is inhibited only at high concentrations of the blockers. Pretreatment of living cells with 1 mM DIDS does not affect membrane adenylate cyclase activity which remains responsive to bicarbonate. These results suggest that controlled transport of bicarbonate through the sperm plasma membrane could be associated to the regulation of cAMP synthesis. © 1990.
author Muschietti, Jorge P.
Flawiá, Mirtha María
Tezón, Jorge G.
author_facet Muschietti, Jorge P.
Flawiá, Mirtha María
Tezón, Jorge G.
author_sort Muschietti, Jorge P.
title Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa
title_short Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa
title_full Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa
title_fullStr Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa
title_full_unstemmed Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa
title_sort bicarbonate dependence of camp accumulation induced by phorbol esters in hamster spermatozoa
publishDate 1990
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674889_v1054_n2_p231_Visconti
http://hdl.handle.net/20.500.12110/paper_01674889_v1054_n2_p231_Visconti
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AT flawiamirthamaria bicarbonatedependenceofcampaccumulationinducedbyphorbolestersinhamsterspermatozoa
AT tezonjorgeg bicarbonatedependenceofcampaccumulationinducedbyphorbolestersinhamsterspermatozoa
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