A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi
A full-length DNA clone encoding a putative pyruvate dehydrogenase α subunit (E1α) gene was isolated from a Trypanosoma cruzi (RA strain) DNA library. Sequencing of this clone revealed it to encode a 378 amino acid protein (M(r) 42774) with high sequence similarity to E1α obtained from different sou...
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1996
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674781_v1309_n1-2_p53_Buscaglia http://hdl.handle.net/20.500.12110/paper_01674781_v1309_n1-2_p53_Buscaglia |
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paper:paper_01674781_v1309_n1-2_p53_Buscaglia2023-06-08T15:16:24Z A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi (Trypanosoma cruzi) Pyruvate dehydrogenase α subunit Trans-splicing pyruvate dehydrogenase amino acid sequence article dna library enzyme subunit gene isolation human molecular cloning nonhuman nucleotide sequence priority journal sequence homology trypanosoma cruzi yeast Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA, Protozoan Gene Dosage Genes, Protozoan Humans Molecular Sequence Data Pyruvate Dehydrogenase Complex RNA, Messenger RNA, Protozoan Sequence Analysis, DNA Sequence Homology, Amino Acid Trypanosoma cruzi Trypanosoma Trypanosoma cruzi A full-length DNA clone encoding a putative pyruvate dehydrogenase α subunit (E1α) gene was isolated from a Trypanosoma cruzi (RA strain) DNA library. Sequencing of this clone revealed it to encode a 378 amino acid protein (M(r) 42774) with high sequence similarity to E1α obtained from different sources. The highest score is obtained with human E1α: 43.3% similarity. Southern blot analysis is consistent with the existence of a single copy of this putative T. cruzi E1α gene per haploid genome in different parasite strains. Expression of this gene was demonstrated by Northern blot analysis and its trans-splicing acceptor site was identified by Polymerase Chain Reaction-mediated amplification of its cDNA. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674781_v1309_n1-2_p53_Buscaglia http://hdl.handle.net/20.500.12110/paper_01674781_v1309_n1-2_p53_Buscaglia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
(Trypanosoma cruzi) Pyruvate dehydrogenase α subunit Trans-splicing pyruvate dehydrogenase amino acid sequence article dna library enzyme subunit gene isolation human molecular cloning nonhuman nucleotide sequence priority journal sequence homology trypanosoma cruzi yeast Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA, Protozoan Gene Dosage Genes, Protozoan Humans Molecular Sequence Data Pyruvate Dehydrogenase Complex RNA, Messenger RNA, Protozoan Sequence Analysis, DNA Sequence Homology, Amino Acid Trypanosoma cruzi Trypanosoma Trypanosoma cruzi |
| spellingShingle |
(Trypanosoma cruzi) Pyruvate dehydrogenase α subunit Trans-splicing pyruvate dehydrogenase amino acid sequence article dna library enzyme subunit gene isolation human molecular cloning nonhuman nucleotide sequence priority journal sequence homology trypanosoma cruzi yeast Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA, Protozoan Gene Dosage Genes, Protozoan Humans Molecular Sequence Data Pyruvate Dehydrogenase Complex RNA, Messenger RNA, Protozoan Sequence Analysis, DNA Sequence Homology, Amino Acid Trypanosoma cruzi Trypanosoma Trypanosoma cruzi A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi |
| topic_facet |
(Trypanosoma cruzi) Pyruvate dehydrogenase α subunit Trans-splicing pyruvate dehydrogenase amino acid sequence article dna library enzyme subunit gene isolation human molecular cloning nonhuman nucleotide sequence priority journal sequence homology trypanosoma cruzi yeast Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA, Protozoan Gene Dosage Genes, Protozoan Humans Molecular Sequence Data Pyruvate Dehydrogenase Complex RNA, Messenger RNA, Protozoan Sequence Analysis, DNA Sequence Homology, Amino Acid Trypanosoma cruzi Trypanosoma Trypanosoma cruzi |
| description |
A full-length DNA clone encoding a putative pyruvate dehydrogenase α subunit (E1α) gene was isolated from a Trypanosoma cruzi (RA strain) DNA library. Sequencing of this clone revealed it to encode a 378 amino acid protein (M(r) 42774) with high sequence similarity to E1α obtained from different sources. The highest score is obtained with human E1α: 43.3% similarity. Southern blot analysis is consistent with the existence of a single copy of this putative T. cruzi E1α gene per haploid genome in different parasite strains. Expression of this gene was demonstrated by Northern blot analysis and its trans-splicing acceptor site was identified by Polymerase Chain Reaction-mediated amplification of its cDNA. |
| title |
A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi |
| title_short |
A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi |
| title_full |
A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi |
| title_fullStr |
A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi |
| title_full_unstemmed |
A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi |
| title_sort |
putative pyruvate dehydrogenase α subunit gene from trypanosoma cruzi |
| publishDate |
1996 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674781_v1309_n1-2_p53_Buscaglia http://hdl.handle.net/20.500.12110/paper_01674781_v1309_n1-2_p53_Buscaglia |
| _version_ |
1768542032310042624 |