Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis

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While studying blue light-independent effects of cryptochrome 1 (cry1) photoreceptor, we observed premature opening of the hook in cry1 mutants grown in complete darkness, a phenotype that resembles the one described for the heterotrimeric G-protein α subunit (GPA1) null mutant gpa1. Both cry1 and g...

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Autores principales:	Fox, Ana Romina, Soto, Gabriela Cynthia, Muschietti, Jorge P., Mazzella, María Agustina
Publicado:	2012
Materias:	Arabidopsis thaliana cry1 GPA1 Heterotrimeric G-proteins Signal transduction anthocyanin Arabidopsis protein CRY1 protein, Arabidopsis cryptochrome GPA1 protein, Arabidopsis guanine nucleotide binding protein alpha subunit messenger RNA plant RNA sucrose Arabidopsis article biological model biosynthesis drug effect gene expression regulation genetics light metabolism mutation phenotype physiology plant growth protein binding protein processing radiation exposure seedling signal transduction Anthocyanins Arabidopsis Proteins Cryptochromes Gene Expression Regulation, Plant GTP-Binding Protein alpha Subunits Hypocotyl Light Models, Biological Mutation Phenotype Protein Binding Protein Processing, Post-Translational RNA, Messenger RNA, Plant Seedling Signal Transduction Sucrose
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674412_v80_n3_p315_Fox http://hdl.handle.net/20.500.12110/paper_01674412_v80_n3_p315_Fox
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01674412_v80_n3_p315_Fox
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spelling	paper:paper_01674412_v80_n3_p315_Fox2023-06-08T15:16:23Z Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis Fox, Ana Romina Soto, Gabriela Cynthia Muschietti, Jorge P. Mazzella, María Agustina Arabidopsis thaliana cry1 GPA1 Heterotrimeric G-proteins Signal transduction anthocyanin Arabidopsis protein CRY1 protein, Arabidopsis cryptochrome GPA1 protein, Arabidopsis guanine nucleotide binding protein alpha subunit messenger RNA plant RNA sucrose Arabidopsis article biological model biosynthesis drug effect gene expression regulation genetics light metabolism mutation phenotype physiology plant growth protein binding protein processing radiation exposure seedling signal transduction Anthocyanins Arabidopsis Arabidopsis Proteins Cryptochromes Gene Expression Regulation, Plant GTP-Binding Protein alpha Subunits Hypocotyl Light Models, Biological Mutation Phenotype Protein Binding Protein Processing, Post-Translational RNA, Messenger RNA, Plant Seedling Signal Transduction Sucrose Arabidopsis Arabidopsis thaliana While studying blue light-independent effects of cryptochrome 1 (cry1) photoreceptor, we observed premature opening of the hook in cry1 mutants grown in complete darkness, a phenotype that resembles the one described for the heterotrimeric G-protein α subunit (GPA1) null mutant gpa1. Both cry1 and gpa1 also showed reduced accumulation of anthocyanin under blue light. These convergent gpa1 and cry1 phenotypes required the presence of sucrose in the growth media and were not additive in the cry1gpa1 double mutant, suggesting context-dependent signaling convergence between cry1 and GPA1 signaling pathways. Both, gpa1 and cry1 mutants showed reduced GTP-binding activity. The cry1 mutant showed wild-type levels of GPA1 mRNA or GPA1 protein. However, an anti-transducin antibody (AS/7) typically used for plant Gα proteins, recognized a 54 kDa band in the wild type but not in gpa1 and cry1 mutants. We propose a model where cry1-mediated post-translational modification of GPA1 alters its GTP-binding activity. © 2012 Springer Science+Business Media B.V. Fil:Fox, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Soto, G.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muschietti, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mazzella, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674412_v80_n3_p315_Fox http://hdl.handle.net/20.500.12110/paper_01674412_v80_n3_p315_Fox
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Arabidopsis thaliana cry1 GPA1 Heterotrimeric G-proteins Signal transduction anthocyanin Arabidopsis protein CRY1 protein, Arabidopsis cryptochrome GPA1 protein, Arabidopsis guanine nucleotide binding protein alpha subunit messenger RNA plant RNA sucrose Arabidopsis article biological model biosynthesis drug effect gene expression regulation genetics light metabolism mutation phenotype physiology plant growth protein binding protein processing radiation exposure seedling signal transduction Anthocyanins Arabidopsis Arabidopsis Proteins Cryptochromes Gene Expression Regulation, Plant GTP-Binding Protein alpha Subunits Hypocotyl Light Models, Biological Mutation Phenotype Protein Binding Protein Processing, Post-Translational RNA, Messenger RNA, Plant Seedling Signal Transduction Sucrose Arabidopsis Arabidopsis thaliana
spellingShingle	Arabidopsis thaliana cry1 GPA1 Heterotrimeric G-proteins Signal transduction anthocyanin Arabidopsis protein CRY1 protein, Arabidopsis cryptochrome GPA1 protein, Arabidopsis guanine nucleotide binding protein alpha subunit messenger RNA plant RNA sucrose Arabidopsis article biological model biosynthesis drug effect gene expression regulation genetics light metabolism mutation phenotype physiology plant growth protein binding protein processing radiation exposure seedling signal transduction Anthocyanins Arabidopsis Arabidopsis Proteins Cryptochromes Gene Expression Regulation, Plant GTP-Binding Protein alpha Subunits Hypocotyl Light Models, Biological Mutation Phenotype Protein Binding Protein Processing, Post-Translational RNA, Messenger RNA, Plant Seedling Signal Transduction Sucrose Arabidopsis Arabidopsis thaliana Fox, Ana Romina Soto, Gabriela Cynthia Muschietti, Jorge P. Mazzella, María Agustina Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis
topic_facet	Arabidopsis thaliana cry1 GPA1 Heterotrimeric G-proteins Signal transduction anthocyanin Arabidopsis protein CRY1 protein, Arabidopsis cryptochrome GPA1 protein, Arabidopsis guanine nucleotide binding protein alpha subunit messenger RNA plant RNA sucrose Arabidopsis article biological model biosynthesis drug effect gene expression regulation genetics light metabolism mutation phenotype physiology plant growth protein binding protein processing radiation exposure seedling signal transduction Anthocyanins Arabidopsis Arabidopsis Proteins Cryptochromes Gene Expression Regulation, Plant GTP-Binding Protein alpha Subunits Hypocotyl Light Models, Biological Mutation Phenotype Protein Binding Protein Processing, Post-Translational RNA, Messenger RNA, Plant Seedling Signal Transduction Sucrose Arabidopsis Arabidopsis thaliana
description	While studying blue light-independent effects of cryptochrome 1 (cry1) photoreceptor, we observed premature opening of the hook in cry1 mutants grown in complete darkness, a phenotype that resembles the one described for the heterotrimeric G-protein α subunit (GPA1) null mutant gpa1. Both cry1 and gpa1 also showed reduced accumulation of anthocyanin under blue light. These convergent gpa1 and cry1 phenotypes required the presence of sucrose in the growth media and were not additive in the cry1gpa1 double mutant, suggesting context-dependent signaling convergence between cry1 and GPA1 signaling pathways. Both, gpa1 and cry1 mutants showed reduced GTP-binding activity. The cry1 mutant showed wild-type levels of GPA1 mRNA or GPA1 protein. However, an anti-transducin antibody (AS/7) typically used for plant Gα proteins, recognized a 54 kDa band in the wild type but not in gpa1 and cry1 mutants. We propose a model where cry1-mediated post-translational modification of GPA1 alters its GTP-binding activity. © 2012 Springer Science+Business Media B.V.
author	Fox, Ana Romina Soto, Gabriela Cynthia Muschietti, Jorge P. Mazzella, María Agustina
author_facet	Fox, Ana Romina Soto, Gabriela Cynthia Muschietti, Jorge P. Mazzella, María Agustina
author_sort	Fox, Ana Romina
title	Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis
title_short	Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis
title_full	Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis
title_fullStr	Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis
title_full_unstemmed	Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis
title_sort	cry1 and gpa1 signaling genetically interact in hook opening and anthocyanin synthesis in arabidopsis
publishDate	2012
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674412_v80_n3_p315_Fox http://hdl.handle.net/20.500.12110/paper_01674412_v80_n3_p315_Fox
work_keys_str_mv	AT foxanaromina cry1andgpa1signalinggeneticallyinteractinhookopeningandanthocyaninsynthesisinarabidopsis AT sotogabrielacynthia cry1andgpa1signalinggeneticallyinteractinhookopeningandanthocyaninsynthesisinarabidopsis AT muschiettijorgep cry1andgpa1signalinggeneticallyinteractinhookopeningandanthocyaninsynthesisinarabidopsis AT mazzellamariaagustina cry1andgpa1signalinggeneticallyinteractinhookopeningandanthocyaninsynthesisinarabidopsis
_version_	1768544035062939648

Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis

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