Developmentally regulated expression of ceramide in Trypanosoma cruzi

Amastigote forms of T. cruzi express the specific Ssp-4 surface antigen which is progressively shed, by the action of an endogenous phosphatidylinositol-phospholipase C, during their development into epimastigotes (Andrews et al., J. Exp. Med., 167 (1988) 300-314). We show now that the lipid moiety...

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Detalles Bibliográficos
Autores principales:	Bertello, Laura Elena, Muchnik de Lederkremer, Rosa María
Publicado:	1996
Materias:	Ceramide GPI-anchor Ssp-4 Trypanosoma cruzi ceramide membrane antigen n palmitoyldihydrosphingosine palmitic acid phosphatidylinositol phospholipase C tritium unclassified drug amastigote article controlled study epimastigote lipid analysis nonhuman priority journal Mammalia Tritium Trypanosoma
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v79_n2_p143_Bertello http://hdl.handle.net/20.500.12110/paper_01666851_v79_n2_p143_Bertello
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01666851_v79_n2_p143_Bertello
record_format	dspace
spelling	paper:paper_01666851_v79_n2_p143_Bertello2023-06-08T15:16:08Z Developmentally regulated expression of ceramide in Trypanosoma cruzi Bertello, Laura Elena Muchnik de Lederkremer, Rosa María Ceramide GPI-anchor Ssp-4 Trypanosoma cruzi ceramide membrane antigen n palmitoyldihydrosphingosine palmitic acid phosphatidylinositol phospholipase C tritium unclassified drug amastigote article controlled study epimastigote lipid analysis nonhuman priority journal Trypanosoma cruzi Mammalia Tritium Trypanosoma Trypanosoma cruzi Amastigote forms of T. cruzi express the specific Ssp-4 surface antigen which is progressively shed, by the action of an endogenous phosphatidylinositol-phospholipase C, during their development into epimastigotes (Andrews et al., J. Exp. Med., 167 (1988) 300-314). We show now that the lipid moiety of the anchor of Ssp-4 is a ceramide which was metabolically labelled with [[3H]palmitic acid. The lipid could be cleaved by PI-PLC digestion in vitro, and was identified by methanolysis and reverse phase thin layer chromatography of the products, as palmitoyldihydrosphingosine. Also, the free biosynthesized lipids were investigated in parasites obtained after 0, 24, 48 and 72 h differentiation of trypomastigotes and further incubated with [3H]palmitic acid for 2 h. A maximum of free ceramide was found in the 24 h point, in accordance with the maximum of amastigote forms. In contrast only traces of free ceramide were found in trypomastigotes. The major ceramide (more than 90%) is palmitoyldihydrosphingosine, which is the same as found in the anchor of Ssp-4. The ceramide could play-an important role in the cell biology of the parasite as previously found for mammalian cells. Fil:Bertello, L.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v79_n2_p143_Bertello http://hdl.handle.net/20.500.12110/paper_01666851_v79_n2_p143_Bertello
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Ceramide GPI-anchor Ssp-4 Trypanosoma cruzi ceramide membrane antigen n palmitoyldihydrosphingosine palmitic acid phosphatidylinositol phospholipase C tritium unclassified drug amastigote article controlled study epimastigote lipid analysis nonhuman priority journal Trypanosoma cruzi Mammalia Tritium Trypanosoma Trypanosoma cruzi
spellingShingle	Ceramide GPI-anchor Ssp-4 Trypanosoma cruzi ceramide membrane antigen n palmitoyldihydrosphingosine palmitic acid phosphatidylinositol phospholipase C tritium unclassified drug amastigote article controlled study epimastigote lipid analysis nonhuman priority journal Trypanosoma cruzi Mammalia Tritium Trypanosoma Trypanosoma cruzi Bertello, Laura Elena Muchnik de Lederkremer, Rosa María Developmentally regulated expression of ceramide in Trypanosoma cruzi
topic_facet	Ceramide GPI-anchor Ssp-4 Trypanosoma cruzi ceramide membrane antigen n palmitoyldihydrosphingosine palmitic acid phosphatidylinositol phospholipase C tritium unclassified drug amastigote article controlled study epimastigote lipid analysis nonhuman priority journal Trypanosoma cruzi Mammalia Tritium Trypanosoma Trypanosoma cruzi
description	Amastigote forms of T. cruzi express the specific Ssp-4 surface antigen which is progressively shed, by the action of an endogenous phosphatidylinositol-phospholipase C, during their development into epimastigotes (Andrews et al., J. Exp. Med., 167 (1988) 300-314). We show now that the lipid moiety of the anchor of Ssp-4 is a ceramide which was metabolically labelled with [[3H]palmitic acid. The lipid could be cleaved by PI-PLC digestion in vitro, and was identified by methanolysis and reverse phase thin layer chromatography of the products, as palmitoyldihydrosphingosine. Also, the free biosynthesized lipids were investigated in parasites obtained after 0, 24, 48 and 72 h differentiation of trypomastigotes and further incubated with [3H]palmitic acid for 2 h. A maximum of free ceramide was found in the 24 h point, in accordance with the maximum of amastigote forms. In contrast only traces of free ceramide were found in trypomastigotes. The major ceramide (more than 90%) is palmitoyldihydrosphingosine, which is the same as found in the anchor of Ssp-4. The ceramide could play-an important role in the cell biology of the parasite as previously found for mammalian cells.
author	Bertello, Laura Elena Muchnik de Lederkremer, Rosa María
author_facet	Bertello, Laura Elena Muchnik de Lederkremer, Rosa María
author_sort	Bertello, Laura Elena
title	Developmentally regulated expression of ceramide in Trypanosoma cruzi
title_short	Developmentally regulated expression of ceramide in Trypanosoma cruzi
title_full	Developmentally regulated expression of ceramide in Trypanosoma cruzi
title_fullStr	Developmentally regulated expression of ceramide in Trypanosoma cruzi
title_full_unstemmed	Developmentally regulated expression of ceramide in Trypanosoma cruzi
title_sort	developmentally regulated expression of ceramide in trypanosoma cruzi
publishDate	1996
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v79_n2_p143_Bertello http://hdl.handle.net/20.500.12110/paper_01666851_v79_n2_p143_Bertello
work_keys_str_mv	AT bertellolauraelena developmentallyregulatedexpressionofceramideintrypanosomacruzi AT muchnikdelederkremerrosamaria developmentallyregulatedexpressionofceramideintrypanosomacruzi
_version_	1768546536097054720

Developmentally regulated expression of ceramide in Trypanosoma cruzi

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