Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi

Highly purified acidocalcisomes from Trypanosoma cruzi epimastigotes were obtained by differential centrifugation and iodixanol gradient ultracentrifugation. Lipid analysis of acidocalcisomes revealed the presence of low amounts of 3β-hydroxysterols and predominance of phospholipids. Alkylacyl phosp...

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Autores principales:	Salto, María Laura, Muchnik de Lederkremer, Rosa María
Publicado:	2008
Materias:	Acidocalcisomes Glycolipids Phospholipids Trypanosoma cruzi 3beta hydroxysterol derivative glycolipid phosphatidylcholine phosphatidylethanolamine phosphatidylinositol phospholipid sterol derivative unclassified drug article controlled study electrospray mass spectrometry epimastigote lipid analysis lipid composition nonhuman priority journal Animals Calcium Centrifugation, Density Gradient Hydrogen-Ion Concentration Organelles Spectrometry, Mass, Electrospray Ionization
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v158_n2_p120_Salto http://hdl.handle.net/20.500.12110/paper_01666851_v158_n2_p120_Salto
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01666851_v158_n2_p120_Salto
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spelling	paper:paper_01666851_v158_n2_p120_Salto2023-06-08T15:16:00Z Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi Salto, María Laura Muchnik de Lederkremer, Rosa María Acidocalcisomes Glycolipids Phospholipids Trypanosoma cruzi 3beta hydroxysterol derivative glycolipid phosphatidylcholine phosphatidylethanolamine phosphatidylinositol phospholipid sterol derivative unclassified drug article controlled study electrospray mass spectrometry epimastigote lipid analysis lipid composition nonhuman priority journal Trypanosoma cruzi Animals Calcium Centrifugation, Density Gradient Glycolipids Hydrogen-Ion Concentration Organelles Phospholipids Spectrometry, Mass, Electrospray Ionization Trypanosoma cruzi Trypanosoma cruzi Highly purified acidocalcisomes from Trypanosoma cruzi epimastigotes were obtained by differential centrifugation and iodixanol gradient ultracentrifugation. Lipid analysis of acidocalcisomes revealed the presence of low amounts of 3β-hydroxysterols and predominance of phospholipids. Alkylacyl phosphatidylinositol (16:0/18:2), diacyl phosphatidylinositol (18:0/18:2), diacyl phosphatidylcholine (16:0/18:2; 16:1/18:2; 16:2/18:2; 18:1/18:2 and 18:2/18:2), and diacyl phosphatidylethanolamine (16:0/18:2 and 16:1/18:2) were the only phospholipids characterized by electrospray ionization-mass spectrometry (ESI-MS). Incubation of epimastigotes with [3H]-mannose and isolation of acidocalcisomes allowed the detection of a glycoinositolphospholipid (GIPL) in these organelles. The sugar content of the acidocalcisomal GIPL was similar to that of the GIPL present in a microsomal fraction but the amount of galactofuranose and inositol with respect to the other monosaccharides was lower, suggesting a different chemical structure. Taken together, these results indicate that acidocalcisomes of T. cruzi have a distinct lipid and carbohydrate composition. © 2007 Elsevier B.V. All rights reserved. Fil:Salto, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:de Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v158_n2_p120_Salto http://hdl.handle.net/20.500.12110/paper_01666851_v158_n2_p120_Salto
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Acidocalcisomes Glycolipids Phospholipids Trypanosoma cruzi 3beta hydroxysterol derivative glycolipid phosphatidylcholine phosphatidylethanolamine phosphatidylinositol phospholipid sterol derivative unclassified drug article controlled study electrospray mass spectrometry epimastigote lipid analysis lipid composition nonhuman priority journal Trypanosoma cruzi Animals Calcium Centrifugation, Density Gradient Glycolipids Hydrogen-Ion Concentration Organelles Phospholipids Spectrometry, Mass, Electrospray Ionization Trypanosoma cruzi Trypanosoma cruzi
spellingShingle	Acidocalcisomes Glycolipids Phospholipids Trypanosoma cruzi 3beta hydroxysterol derivative glycolipid phosphatidylcholine phosphatidylethanolamine phosphatidylinositol phospholipid sterol derivative unclassified drug article controlled study electrospray mass spectrometry epimastigote lipid analysis lipid composition nonhuman priority journal Trypanosoma cruzi Animals Calcium Centrifugation, Density Gradient Glycolipids Hydrogen-Ion Concentration Organelles Phospholipids Spectrometry, Mass, Electrospray Ionization Trypanosoma cruzi Trypanosoma cruzi Salto, María Laura Muchnik de Lederkremer, Rosa María Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi
topic_facet	Acidocalcisomes Glycolipids Phospholipids Trypanosoma cruzi 3beta hydroxysterol derivative glycolipid phosphatidylcholine phosphatidylethanolamine phosphatidylinositol phospholipid sterol derivative unclassified drug article controlled study electrospray mass spectrometry epimastigote lipid analysis lipid composition nonhuman priority journal Trypanosoma cruzi Animals Calcium Centrifugation, Density Gradient Glycolipids Hydrogen-Ion Concentration Organelles Phospholipids Spectrometry, Mass, Electrospray Ionization Trypanosoma cruzi Trypanosoma cruzi
description	Highly purified acidocalcisomes from Trypanosoma cruzi epimastigotes were obtained by differential centrifugation and iodixanol gradient ultracentrifugation. Lipid analysis of acidocalcisomes revealed the presence of low amounts of 3β-hydroxysterols and predominance of phospholipids. Alkylacyl phosphatidylinositol (16:0/18:2), diacyl phosphatidylinositol (18:0/18:2), diacyl phosphatidylcholine (16:0/18:2; 16:1/18:2; 16:2/18:2; 18:1/18:2 and 18:2/18:2), and diacyl phosphatidylethanolamine (16:0/18:2 and 16:1/18:2) were the only phospholipids characterized by electrospray ionization-mass spectrometry (ESI-MS). Incubation of epimastigotes with [3H]-mannose and isolation of acidocalcisomes allowed the detection of a glycoinositolphospholipid (GIPL) in these organelles. The sugar content of the acidocalcisomal GIPL was similar to that of the GIPL present in a microsomal fraction but the amount of galactofuranose and inositol with respect to the other monosaccharides was lower, suggesting a different chemical structure. Taken together, these results indicate that acidocalcisomes of T. cruzi have a distinct lipid and carbohydrate composition. © 2007 Elsevier B.V. All rights reserved.
author	Salto, María Laura Muchnik de Lederkremer, Rosa María
author_facet	Salto, María Laura Muchnik de Lederkremer, Rosa María
author_sort	Salto, María Laura
title	Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi
title_short	Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi
title_full	Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi
title_fullStr	Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi
title_full_unstemmed	Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi
title_sort	phospholipid and glycolipid composition of acidocalcisomes of trypanosoma cruzi
publishDate	2008
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v158_n2_p120_Salto http://hdl.handle.net/20.500.12110/paper_01666851_v158_n2_p120_Salto
work_keys_str_mv	AT saltomarialaura phospholipidandglycolipidcompositionofacidocalcisomesoftrypanosomacruzi AT muchnikdelederkremerrosamaria phospholipidandglycolipidcompositionofacidocalcisomesoftrypanosomacruzi
_version_	1768546488717148160

Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi

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