Evidence for CRK3 participation in the cell division cycle of Trypanosoma cruzi

Trypanosoma cruzi CRK3 gene encodes a Cdc2p related protein kinase (CRK). To establish if it has a role in the regulation of the parasite cell cycle we studied CRK3 expression and activity throughout three life cycle stages. CRK3 from epimastigote soluble extracts interacted with p13suc1-beads. Endo...

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Autores principales:	Santori, María Isabel, Laría, Sebastián, Gómez, Eliana Beatriz
Publicado:	2002
Materias:	Cell cycle control Cyclin-dependent kinase Flavopiridol Hydroxyurea synchronization Trypanosoma cruzi flavopiridol histone H1 hydroxyurea olomoucine protein protein kinase protein kinase inhibitor protein p13 roscovitine unclassified drug article cell cycle cell cycle G2 phase cell division cell synchronization concentration response developmental stage drug effect enzyme activity enzyme binding epimastigote gene expression growth inhibition mitosis inhibition nonhuman parasite development phosphorylation priority journal protein interaction regulatory mechanism Animals CDC2 Protein Kinase Cell Cycle Cyclin-Dependent Kinases Enzyme Inhibitors Flavonoids Gene Expression Regulation Hydroxyurea Piperidines Protozoan Proteins Trypanosoma
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v121_n2_p225_Santori http://hdl.handle.net/20.500.12110/paper_01666851_v121_n2_p225_Santori
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01666851_v121_n2_p225_Santori
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spelling	paper:paper_01666851_v121_n2_p225_Santori2023-06-08T15:15:52Z Evidence for CRK3 participation in the cell division cycle of Trypanosoma cruzi Santori, María Isabel Laría, Sebastián Gómez, Eliana Beatriz Cell cycle control Cyclin-dependent kinase Flavopiridol Hydroxyurea synchronization Trypanosoma cruzi flavopiridol histone H1 hydroxyurea olomoucine protein protein kinase protein kinase inhibitor protein p13 roscovitine unclassified drug article cell cycle cell cycle G2 phase cell division cell synchronization concentration response developmental stage drug effect enzyme activity enzyme binding epimastigote gene expression growth inhibition mitosis inhibition nonhuman parasite development phosphorylation priority journal protein interaction regulatory mechanism Trypanosoma cruzi Animals CDC2 Protein Kinase Cell Cycle Cyclin-Dependent Kinases Enzyme Inhibitors Flavonoids Gene Expression Regulation Hydroxyurea Piperidines Protozoan Proteins Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Trypanosoma cruzi CRK3 gene encodes a Cdc2p related protein kinase (CRK). To establish if it has a role in the regulation of the parasite cell cycle we studied CRK3 expression and activity throughout three life cycle stages. CRK3 from epimastigote soluble extracts interacted with p13suc1-beads. Endogenous CRK3 phosphorylated histone H1 and this activity was inhibited by specific CDK inhibitors: Olomoucine, Flavopiridol and Roscovitine. Flavopiridol partially inhibited the growth of T. cruzi epimastigotes at 50 nM, the lowest concentration used, but even with the highest (5 μM), cell growth was not completely arrested. CRK3 from Flavopiridol-inhibited epimastigote extracts exhibited a dose dependent inhibition of histone H1 phosphorylation. T. cruzi p13suc1-binding CRK displayed the same inhibition profile. This suggests that CRK3 is the enzyme responsible for the majority of the kinase activity associated with p13suc1. CRK3 activity of hydroxyurea (HU) synchronized epimastigotes peaked in G2/M boundary while the kinase activity associated to p13suc1-beads increased at the same time point but remained high until late G2/M. In addition, CRK3 expression was constant during the cell cycle. This is a common pattern of CDK activity regulation. Taken together, these results support the idea that CRK3 is involved in control of the cell cycle in T. cruzi. © 2002 Elsevier Science B.V. All rights reserved. Fil:Santori, M.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Laría, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gómez, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v121_n2_p225_Santori http://hdl.handle.net/20.500.12110/paper_01666851_v121_n2_p225_Santori
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cell cycle control Cyclin-dependent kinase Flavopiridol Hydroxyurea synchronization Trypanosoma cruzi flavopiridol histone H1 hydroxyurea olomoucine protein protein kinase protein kinase inhibitor protein p13 roscovitine unclassified drug article cell cycle cell cycle G2 phase cell division cell synchronization concentration response developmental stage drug effect enzyme activity enzyme binding epimastigote gene expression growth inhibition mitosis inhibition nonhuman parasite development phosphorylation priority journal protein interaction regulatory mechanism Trypanosoma cruzi Animals CDC2 Protein Kinase Cell Cycle Cyclin-Dependent Kinases Enzyme Inhibitors Flavonoids Gene Expression Regulation Hydroxyurea Piperidines Protozoan Proteins Trypanosoma cruzi Trypanosoma Trypanosoma cruzi
spellingShingle	Cell cycle control Cyclin-dependent kinase Flavopiridol Hydroxyurea synchronization Trypanosoma cruzi flavopiridol histone H1 hydroxyurea olomoucine protein protein kinase protein kinase inhibitor protein p13 roscovitine unclassified drug article cell cycle cell cycle G2 phase cell division cell synchronization concentration response developmental stage drug effect enzyme activity enzyme binding epimastigote gene expression growth inhibition mitosis inhibition nonhuman parasite development phosphorylation priority journal protein interaction regulatory mechanism Trypanosoma cruzi Animals CDC2 Protein Kinase Cell Cycle Cyclin-Dependent Kinases Enzyme Inhibitors Flavonoids Gene Expression Regulation Hydroxyurea Piperidines Protozoan Proteins Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Santori, María Isabel Laría, Sebastián Gómez, Eliana Beatriz Evidence for CRK3 participation in the cell division cycle of Trypanosoma cruzi
topic_facet	Cell cycle control Cyclin-dependent kinase Flavopiridol Hydroxyurea synchronization Trypanosoma cruzi flavopiridol histone H1 hydroxyurea olomoucine protein protein kinase protein kinase inhibitor protein p13 roscovitine unclassified drug article cell cycle cell cycle G2 phase cell division cell synchronization concentration response developmental stage drug effect enzyme activity enzyme binding epimastigote gene expression growth inhibition mitosis inhibition nonhuman parasite development phosphorylation priority journal protein interaction regulatory mechanism Trypanosoma cruzi Animals CDC2 Protein Kinase Cell Cycle Cyclin-Dependent Kinases Enzyme Inhibitors Flavonoids Gene Expression Regulation Hydroxyurea Piperidines Protozoan Proteins Trypanosoma cruzi Trypanosoma Trypanosoma cruzi
description	Trypanosoma cruzi CRK3 gene encodes a Cdc2p related protein kinase (CRK). To establish if it has a role in the regulation of the parasite cell cycle we studied CRK3 expression and activity throughout three life cycle stages. CRK3 from epimastigote soluble extracts interacted with p13suc1-beads. Endogenous CRK3 phosphorylated histone H1 and this activity was inhibited by specific CDK inhibitors: Olomoucine, Flavopiridol and Roscovitine. Flavopiridol partially inhibited the growth of T. cruzi epimastigotes at 50 nM, the lowest concentration used, but even with the highest (5 μM), cell growth was not completely arrested. CRK3 from Flavopiridol-inhibited epimastigote extracts exhibited a dose dependent inhibition of histone H1 phosphorylation. T. cruzi p13suc1-binding CRK displayed the same inhibition profile. This suggests that CRK3 is the enzyme responsible for the majority of the kinase activity associated with p13suc1. CRK3 activity of hydroxyurea (HU) synchronized epimastigotes peaked in G2/M boundary while the kinase activity associated to p13suc1-beads increased at the same time point but remained high until late G2/M. In addition, CRK3 expression was constant during the cell cycle. This is a common pattern of CDK activity regulation. Taken together, these results support the idea that CRK3 is involved in control of the cell cycle in T. cruzi. © 2002 Elsevier Science B.V. All rights reserved.
author	Santori, María Isabel Laría, Sebastián Gómez, Eliana Beatriz
author_facet	Santori, María Isabel Laría, Sebastián Gómez, Eliana Beatriz
author_sort	Santori, María Isabel
title	Evidence for CRK3 participation in the cell division cycle of Trypanosoma cruzi
title_short	Evidence for CRK3 participation in the cell division cycle of Trypanosoma cruzi
title_full	Evidence for CRK3 participation in the cell division cycle of Trypanosoma cruzi
title_fullStr	Evidence for CRK3 participation in the cell division cycle of Trypanosoma cruzi
title_full_unstemmed	Evidence for CRK3 participation in the cell division cycle of Trypanosoma cruzi
title_sort	evidence for crk3 participation in the cell division cycle of trypanosoma cruzi
publishDate	2002
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v121_n2_p225_Santori http://hdl.handle.net/20.500.12110/paper_01666851_v121_n2_p225_Santori
work_keys_str_mv	AT santorimariaisabel evidenceforcrk3participationinthecelldivisioncycleoftrypanosomacruzi AT lariasebastian evidenceforcrk3participationinthecelldivisioncycleoftrypanosomacruzi AT gomezelianabeatriz evidenceforcrk3participationinthecelldivisioncycleoftrypanosomacruzi
_version_	1768542548116111360

Evidence for CRK3 participation in the cell division cycle of Trypanosoma cruzi

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