Phosphatidylcholine formation is the predominant lipid biosynthetic event in the hemoparasite Babesia bovis
This work examines the lipid composition and metabolism of bovine red blood cells infected by apicomplexan Babesia parasites, organisms closely related to Plasmodium sp. We found that erythrocytes infected with Babesia bovis (i-RBC) accumulate lipids and show striking increases in phosphatidylcholin...
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2000
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v106_n1_p147_FlorinChristensen http://hdl.handle.net/20.500.12110/paper_01666851_v106_n1_p147_FlorinChristensen |
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paper:paper_01666851_v106_n1_p147_FlorinChristensen |
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dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Babesia Erythrocyte Lipid Metabolism Phosphatidylcholine Phosphatidylinositol carbon 14 cholesterol cholesterol ester diacylglycerol lipid lysophosphatidylcholine phosphatidic acid phosphatidylcholine phospholipase A2 sphingomyelin stearic acid animal cell article Babesia bovis babesiosis cattle cell culture controlled study erythrocyte gas chromatography hemolysis isotope labeling lipid composition lipid metabolism lipid storage lipogenesis mass spectrometry merozoite nonhuman priority journal Animals Babesia bovis Carbon Radioisotopes Cattle Cells, Cultured Cholesterol Esters Chromatography, Thin Layer Diglycerides Erythrocytes Gas Chromatography-Mass Spectrometry Hemolysis Iodine Radioisotopes Lipid Metabolism Lipids Phosphatidic Acids Phosphatidylcholines Phosphatidylinositols Phospholipases A |
| spellingShingle |
Babesia Erythrocyte Lipid Metabolism Phosphatidylcholine Phosphatidylinositol carbon 14 cholesterol cholesterol ester diacylglycerol lipid lysophosphatidylcholine phosphatidic acid phosphatidylcholine phospholipase A2 sphingomyelin stearic acid animal cell article Babesia bovis babesiosis cattle cell culture controlled study erythrocyte gas chromatography hemolysis isotope labeling lipid composition lipid metabolism lipid storage lipogenesis mass spectrometry merozoite nonhuman priority journal Animals Babesia bovis Carbon Radioisotopes Cattle Cells, Cultured Cholesterol Esters Chromatography, Thin Layer Diglycerides Erythrocytes Gas Chromatography-Mass Spectrometry Hemolysis Iodine Radioisotopes Lipid Metabolism Lipids Phosphatidic Acids Phosphatidylcholines Phosphatidylinositols Phospholipases A Florin-Christensen, Jorge Jacobsen de Florín-Christensen, Mónica O. Phosphatidylcholine formation is the predominant lipid biosynthetic event in the hemoparasite Babesia bovis |
| topic_facet |
Babesia Erythrocyte Lipid Metabolism Phosphatidylcholine Phosphatidylinositol carbon 14 cholesterol cholesterol ester diacylglycerol lipid lysophosphatidylcholine phosphatidic acid phosphatidylcholine phospholipase A2 sphingomyelin stearic acid animal cell article Babesia bovis babesiosis cattle cell culture controlled study erythrocyte gas chromatography hemolysis isotope labeling lipid composition lipid metabolism lipid storage lipogenesis mass spectrometry merozoite nonhuman priority journal Animals Babesia bovis Carbon Radioisotopes Cattle Cells, Cultured Cholesterol Esters Chromatography, Thin Layer Diglycerides Erythrocytes Gas Chromatography-Mass Spectrometry Hemolysis Iodine Radioisotopes Lipid Metabolism Lipids Phosphatidic Acids Phosphatidylcholines Phosphatidylinositols Phospholipases A |
| description |
This work examines the lipid composition and metabolism of bovine red blood cells infected by apicomplexan Babesia parasites, organisms closely related to Plasmodium sp. We found that erythrocytes infected with Babesia bovis (i-RBC) accumulate lipids and show striking increases in phosphatidylcholine, phosphatidic acid, diacylglycerol and cholesteryl esters as compared to uninfected erythrocytes cultured under the same conditions (n- RBC). A similar pattern was observed in cultures of erythrocytes infected with Babesia bigemina. The lipid profile of purified B. bovis merozoites showed that phosphatidylcholine is the most abundant phospholipid in this parasite (31.8% ± 2.8 of total phospholipid), markedly differing from bovine n-RBC, in which it is only a minor component (4.8% ± 0.6). B. bovis cultures incorporate radiolabeled choline into complex lipids, especially phosphatidylcholine, with minor amounts recovered in sphingomyelin and lysophosphatidylcholine. When [14C] stearate was used as precursor, the labeling pattern again gave the highest incorporation into phosphatidylcholine, with lesser incorporation in sphingomyelin, phosphatidylinositol, phosphatidylethanolamine and phosphatidic acid. Diacylglycerol and small amounts of cholesteryl esters were the only labeled neutral lipids found. B. bovis also incorporates [3H] myo-inositol into phosphatidylinositol. Parallel incubations with n-RBC as a control yielded no incorporation into either polar or neutral lipids with any precursor. These results indicate that the lipid changes observed in i-RBC can be explained on the basis of the lipid biosynthetic activities of the babesial parasite. Gas chromatography-mass spectrometry (GC-MS) analysis of fatty acid methyl esters from phospholipids of i-RBC and n-RBC showed the same qualitative composition in both. However, i-RBC had higher ratios of saturated to unsaturated fatty acids and B. bovis cultures did not desaturate [14C] stearate. Cholesterol was the only sterol detected by GC-MS. Phospholipase A2 treatment of i-RBC and n-RBC revealed no enhanced hemolytic effects in i-RBC, suggesting that the erythrocyte membrane phospholipid composition is essentially unaltered by the parasite. Labeling of i-RBC or n-RBC with [125I] Bolton-Hunter resulted in an enhanced phosphatidylserine labeling in i-RBC. This study provides the first data on B. bovis lipid constitution and biosynthesis. They show that phosphatidylcholine formation is the main biosynthetic process in these cells. The striking differences in the contents of phosphatidylcholine between host erythrocytes and the parasite suggests that it may be a useful target for both chemotherapy and immunoprophylaxis against bovine babesiosis. (C) 2000 Elsevier Science B.V. |
| author |
Florin-Christensen, Jorge Jacobsen de Florín-Christensen, Mónica O. |
| author_facet |
Florin-Christensen, Jorge Jacobsen de Florín-Christensen, Mónica O. |
| author_sort |
Florin-Christensen, Jorge |
| title |
Phosphatidylcholine formation is the predominant lipid biosynthetic event in the hemoparasite Babesia bovis |
| title_short |
Phosphatidylcholine formation is the predominant lipid biosynthetic event in the hemoparasite Babesia bovis |
| title_full |
Phosphatidylcholine formation is the predominant lipid biosynthetic event in the hemoparasite Babesia bovis |
| title_fullStr |
Phosphatidylcholine formation is the predominant lipid biosynthetic event in the hemoparasite Babesia bovis |
| title_full_unstemmed |
Phosphatidylcholine formation is the predominant lipid biosynthetic event in the hemoparasite Babesia bovis |
| title_sort |
phosphatidylcholine formation is the predominant lipid biosynthetic event in the hemoparasite babesia bovis |
| publishDate |
2000 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v106_n1_p147_FlorinChristensen http://hdl.handle.net/20.500.12110/paper_01666851_v106_n1_p147_FlorinChristensen |
| work_keys_str_mv |
AT florinchristensenjorge phosphatidylcholineformationisthepredominantlipidbiosyntheticeventinthehemoparasitebabesiabovis AT jacobsendeflorinchristensenmonicao phosphatidylcholineformationisthepredominantlipidbiosyntheticeventinthehemoparasitebabesiabovis |
| _version_ |
1768546394042269696 |
| spelling |
paper:paper_01666851_v106_n1_p147_FlorinChristensen2023-06-08T15:15:51Z Phosphatidylcholine formation is the predominant lipid biosynthetic event in the hemoparasite Babesia bovis Florin-Christensen, Jorge Jacobsen de Florín-Christensen, Mónica O. Babesia Erythrocyte Lipid Metabolism Phosphatidylcholine Phosphatidylinositol carbon 14 cholesterol cholesterol ester diacylglycerol lipid lysophosphatidylcholine phosphatidic acid phosphatidylcholine phospholipase A2 sphingomyelin stearic acid animal cell article Babesia bovis babesiosis cattle cell culture controlled study erythrocyte gas chromatography hemolysis isotope labeling lipid composition lipid metabolism lipid storage lipogenesis mass spectrometry merozoite nonhuman priority journal Animals Babesia bovis Carbon Radioisotopes Cattle Cells, Cultured Cholesterol Esters Chromatography, Thin Layer Diglycerides Erythrocytes Gas Chromatography-Mass Spectrometry Hemolysis Iodine Radioisotopes Lipid Metabolism Lipids Phosphatidic Acids Phosphatidylcholines Phosphatidylinositols Phospholipases A This work examines the lipid composition and metabolism of bovine red blood cells infected by apicomplexan Babesia parasites, organisms closely related to Plasmodium sp. We found that erythrocytes infected with Babesia bovis (i-RBC) accumulate lipids and show striking increases in phosphatidylcholine, phosphatidic acid, diacylglycerol and cholesteryl esters as compared to uninfected erythrocytes cultured under the same conditions (n- RBC). A similar pattern was observed in cultures of erythrocytes infected with Babesia bigemina. The lipid profile of purified B. bovis merozoites showed that phosphatidylcholine is the most abundant phospholipid in this parasite (31.8% ± 2.8 of total phospholipid), markedly differing from bovine n-RBC, in which it is only a minor component (4.8% ± 0.6). B. bovis cultures incorporate radiolabeled choline into complex lipids, especially phosphatidylcholine, with minor amounts recovered in sphingomyelin and lysophosphatidylcholine. When [14C] stearate was used as precursor, the labeling pattern again gave the highest incorporation into phosphatidylcholine, with lesser incorporation in sphingomyelin, phosphatidylinositol, phosphatidylethanolamine and phosphatidic acid. Diacylglycerol and small amounts of cholesteryl esters were the only labeled neutral lipids found. B. bovis also incorporates [3H] myo-inositol into phosphatidylinositol. Parallel incubations with n-RBC as a control yielded no incorporation into either polar or neutral lipids with any precursor. These results indicate that the lipid changes observed in i-RBC can be explained on the basis of the lipid biosynthetic activities of the babesial parasite. Gas chromatography-mass spectrometry (GC-MS) analysis of fatty acid methyl esters from phospholipids of i-RBC and n-RBC showed the same qualitative composition in both. However, i-RBC had higher ratios of saturated to unsaturated fatty acids and B. bovis cultures did not desaturate [14C] stearate. Cholesterol was the only sterol detected by GC-MS. Phospholipase A2 treatment of i-RBC and n-RBC revealed no enhanced hemolytic effects in i-RBC, suggesting that the erythrocyte membrane phospholipid composition is essentially unaltered by the parasite. Labeling of i-RBC or n-RBC with [125I] Bolton-Hunter resulted in an enhanced phosphatidylserine labeling in i-RBC. This study provides the first data on B. bovis lipid constitution and biosynthesis. They show that phosphatidylcholine formation is the main biosynthetic process in these cells. The striking differences in the contents of phosphatidylcholine between host erythrocytes and the parasite suggests that it may be a useful target for both chemotherapy and immunoprophylaxis against bovine babesiosis. (C) 2000 Elsevier Science B.V. Fil:Florin-Christensen, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Florin-Christensen, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2000 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v106_n1_p147_FlorinChristensen http://hdl.handle.net/20.500.12110/paper_01666851_v106_n1_p147_FlorinChristensen |