Cholinesterase and carboxylesterase inhibition in Planorbarius corneus exposed to binary mixtures of azinphos-methyl and chlorpyrifos

Though pesticide mixtures are commonly encountered in fresh water systems, the knowledge of their effects on non-target aquatic species is scarce. The present investigation was undertaken to explore the in vivo inhibition of the freshwater gastropod snail Planorbarius corneus cholinesterase (ChE) an...

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Autores principales:	Verrengia Guerrero, Noemí Rosario, Cochón, Adriana Cristina
Publicado:	2013
Materias:	Carboxylesterase Cholinesterase Invertebrate Mixtures Pesticides 1 naphthyl acetate 2 naphthyl acetate 4 nitrophenyl acetate acetic acid derivative acetylthiocholine azinphos methyl butyric acid 4 nitrophenyl ester butyric acid derivative carboxylesterase chlorpyrifos cholinesterase unclassified drug concentration (composition) detoxification enzyme gastropod inhibition metabolism pesticide snail animal experiment animal tissue article chemical interaction controlled study enzyme activity enzyme inhibition hemolymph in vivo study nonhuman Planorbarius corneus priority journal sensitivity analysis soft tissue Animals Carboxylic Ester Hydrolases Chlorpyrifos Cholinesterases Enzyme Activation Gastropoda Water Pollutants, Chemical Animalia Invertebrata
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v128-129_n_p124_Cacciatore http://hdl.handle.net/20.500.12110/paper_0166445X_v128-129_n_p124_Cacciatore
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_0166445X_v128-129_n_p124_Cacciatore2023-06-08T15:15:46Z Cholinesterase and carboxylesterase inhibition in Planorbarius corneus exposed to binary mixtures of azinphos-methyl and chlorpyrifos Verrengia Guerrero, Noemí Rosario Cochón, Adriana Cristina Carboxylesterase Cholinesterase Invertebrate Mixtures Pesticides 1 naphthyl acetate 2 naphthyl acetate 4 nitrophenyl acetate acetic acid derivative acetylthiocholine azinphos methyl butyric acid 4 nitrophenyl ester butyric acid derivative carboxylesterase chlorpyrifos cholinesterase unclassified drug concentration (composition) detoxification enzyme gastropod inhibition metabolism pesticide snail animal experiment animal tissue article chemical interaction controlled study enzyme activity enzyme inhibition hemolymph in vivo study nonhuman Planorbarius corneus priority journal sensitivity analysis snail soft tissue Animals Carboxylic Ester Hydrolases Chlorpyrifos Cholinesterases Enzyme Activation Gastropoda Water Pollutants, Chemical Animalia Gastropoda Invertebrata Planorbarius corneus Though pesticide mixtures are commonly encountered in fresh water systems, the knowledge of their effects on non-target aquatic species is scarce. The present investigation was undertaken to explore the in vivo inhibition of the freshwater gastropod snail Planorbarius corneus cholinesterase (ChE) and carboxylesterases (CES) activities by the organophosphorus pesticides azinphos-methyl (AZM) and chlorpyrifos (CPF). To this end, snails were exposed for 48. h to different concentrations of AZM and CPF in single-chemical and binary-mixture studies, and ChE and CES activities were measured in the whole soft body tissues and hemolymph. ChE activity was measured with acetylthiocholine as substrate and CES activity was measured with four substrates: p-nitrophenyl acetate, p-nitrophenyl butyrate, 1- and 2-naphthyl acetate. Single-chemical experiments showed that CPF was a more potent inhibitor of ChE activity than AZM (350 and 27 times for the whole soft tissue and hemolymph, respectively). CES were 15 times more sensitive than ChE when the activities were measured in the whole soft tissue of the animals exposed to AZM. Based on a default assumption of concentration addition, P. corneus snails were exposed to mixtures of AZM + CPF designed to yield predicted soft tissue ChE inhibitions of 31% (mixture 1), 50% (mixture 2) and 61% (mixture 3). Results showed that ChE inhibition produced by mixture 1 followed a model of concentration addition. In contrast, the other mixtures showed synergism, both in whole soft tissue and hemolymph. In addition, results obtained when the snails were exposed sequentially to the pesticides showed that the sequence AZM/CPF produced at 48. h a higher ChE inhibition than the sequence CPF/CPF. A range of metabolic pathways and responses associated with bioactivation or detoxification may play important roles in organophosphorus interactions. In particular, the data presented in the present study indicate that CES enzymes would be important factors in determining the effects of the mixtures of OPs on P. corneus ChE activity. © 2012 Elsevier B.V. Fil:Verrengia Guerrero, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cochón, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v128-129_n_p124_Cacciatore http://hdl.handle.net/20.500.12110/paper_0166445X_v128-129_n_p124_Cacciatore
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Carboxylesterase Cholinesterase Invertebrate Mixtures Pesticides 1 naphthyl acetate 2 naphthyl acetate 4 nitrophenyl acetate acetic acid derivative acetylthiocholine azinphos methyl butyric acid 4 nitrophenyl ester butyric acid derivative carboxylesterase chlorpyrifos cholinesterase unclassified drug concentration (composition) detoxification enzyme gastropod inhibition metabolism pesticide snail animal experiment animal tissue article chemical interaction controlled study enzyme activity enzyme inhibition hemolymph in vivo study nonhuman Planorbarius corneus priority journal sensitivity analysis snail soft tissue Animals Carboxylic Ester Hydrolases Chlorpyrifos Cholinesterases Enzyme Activation Gastropoda Water Pollutants, Chemical Animalia Gastropoda Invertebrata Planorbarius corneus
spellingShingle	Carboxylesterase Cholinesterase Invertebrate Mixtures Pesticides 1 naphthyl acetate 2 naphthyl acetate 4 nitrophenyl acetate acetic acid derivative acetylthiocholine azinphos methyl butyric acid 4 nitrophenyl ester butyric acid derivative carboxylesterase chlorpyrifos cholinesterase unclassified drug concentration (composition) detoxification enzyme gastropod inhibition metabolism pesticide snail animal experiment animal tissue article chemical interaction controlled study enzyme activity enzyme inhibition hemolymph in vivo study nonhuman Planorbarius corneus priority journal sensitivity analysis snail soft tissue Animals Carboxylic Ester Hydrolases Chlorpyrifos Cholinesterases Enzyme Activation Gastropoda Water Pollutants, Chemical Animalia Gastropoda Invertebrata Planorbarius corneus Verrengia Guerrero, Noemí Rosario Cochón, Adriana Cristina Cholinesterase and carboxylesterase inhibition in Planorbarius corneus exposed to binary mixtures of azinphos-methyl and chlorpyrifos
topic_facet	Carboxylesterase Cholinesterase Invertebrate Mixtures Pesticides 1 naphthyl acetate 2 naphthyl acetate 4 nitrophenyl acetate acetic acid derivative acetylthiocholine azinphos methyl butyric acid 4 nitrophenyl ester butyric acid derivative carboxylesterase chlorpyrifos cholinesterase unclassified drug concentration (composition) detoxification enzyme gastropod inhibition metabolism pesticide snail animal experiment animal tissue article chemical interaction controlled study enzyme activity enzyme inhibition hemolymph in vivo study nonhuman Planorbarius corneus priority journal sensitivity analysis snail soft tissue Animals Carboxylic Ester Hydrolases Chlorpyrifos Cholinesterases Enzyme Activation Gastropoda Water Pollutants, Chemical Animalia Gastropoda Invertebrata Planorbarius corneus
description	Though pesticide mixtures are commonly encountered in fresh water systems, the knowledge of their effects on non-target aquatic species is scarce. The present investigation was undertaken to explore the in vivo inhibition of the freshwater gastropod snail Planorbarius corneus cholinesterase (ChE) and carboxylesterases (CES) activities by the organophosphorus pesticides azinphos-methyl (AZM) and chlorpyrifos (CPF). To this end, snails were exposed for 48. h to different concentrations of AZM and CPF in single-chemical and binary-mixture studies, and ChE and CES activities were measured in the whole soft body tissues and hemolymph. ChE activity was measured with acetylthiocholine as substrate and CES activity was measured with four substrates: p-nitrophenyl acetate, p-nitrophenyl butyrate, 1- and 2-naphthyl acetate. Single-chemical experiments showed that CPF was a more potent inhibitor of ChE activity than AZM (350 and 27 times for the whole soft tissue and hemolymph, respectively). CES were 15 times more sensitive than ChE when the activities were measured in the whole soft tissue of the animals exposed to AZM. Based on a default assumption of concentration addition, P. corneus snails were exposed to mixtures of AZM + CPF designed to yield predicted soft tissue ChE inhibitions of 31% (mixture 1), 50% (mixture 2) and 61% (mixture 3). Results showed that ChE inhibition produced by mixture 1 followed a model of concentration addition. In contrast, the other mixtures showed synergism, both in whole soft tissue and hemolymph. In addition, results obtained when the snails were exposed sequentially to the pesticides showed that the sequence AZM/CPF produced at 48. h a higher ChE inhibition than the sequence CPF/CPF. A range of metabolic pathways and responses associated with bioactivation or detoxification may play important roles in organophosphorus interactions. In particular, the data presented in the present study indicate that CES enzymes would be important factors in determining the effects of the mixtures of OPs on P. corneus ChE activity. © 2012 Elsevier B.V.
author	Verrengia Guerrero, Noemí Rosario Cochón, Adriana Cristina
author_facet	Verrengia Guerrero, Noemí Rosario Cochón, Adriana Cristina
author_sort	Verrengia Guerrero, Noemí Rosario
title	Cholinesterase and carboxylesterase inhibition in Planorbarius corneus exposed to binary mixtures of azinphos-methyl and chlorpyrifos
title_short	Cholinesterase and carboxylesterase inhibition in Planorbarius corneus exposed to binary mixtures of azinphos-methyl and chlorpyrifos
title_full	Cholinesterase and carboxylesterase inhibition in Planorbarius corneus exposed to binary mixtures of azinphos-methyl and chlorpyrifos
title_fullStr	Cholinesterase and carboxylesterase inhibition in Planorbarius corneus exposed to binary mixtures of azinphos-methyl and chlorpyrifos
title_full_unstemmed	Cholinesterase and carboxylesterase inhibition in Planorbarius corneus exposed to binary mixtures of azinphos-methyl and chlorpyrifos
title_sort	cholinesterase and carboxylesterase inhibition in planorbarius corneus exposed to binary mixtures of azinphos-methyl and chlorpyrifos
publishDate	2013
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0166445X_v128-129_n_p124_Cacciatore http://hdl.handle.net/20.500.12110/paper_0166445X_v128-129_n_p124_Cacciatore
work_keys_str_mv	AT verrengiaguerreronoemirosario cholinesteraseandcarboxylesteraseinhibitioninplanorbariuscorneusexposedtobinarymixturesofazinphosmethylandchlorpyrifos AT cochonadrianacristina cholinesteraseandcarboxylesteraseinhibitioninplanorbariuscorneusexposedtobinarymixturesofazinphosmethylandchlorpyrifos
_version_	1768544636050079744

Cholinesterase and carboxylesterase inhibition in Planorbarius corneus exposed to binary mixtures of azinphos-methyl and chlorpyrifos

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