Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L

A partially purified plant inhibitor (Meliacin) isolated from Melia azedarach L induced in cells a refractory state to virus infection. Meliacin was active in a large variety of continuous and/or primary cell cultures. A state of maximum virus resistance was achieved after 2 h of incubation and was...

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Autores principales:	Andrei, Graciela Mónica, Damonte, Elsa Beatriz, Coto, Celia Esther
Publicado:	1988
Materias:	Antiviral state Melia azedarach L Plant antiviral dactinomycin meliacine unclassified drug cell culture chicken drug mechanism nonhuman plant plant leaf priority journal virus infection Animal Antiviral Agents Cells, Cultured Human Interferons Kinetics Plants Support, Non-U.S. Gov't Vesicular stomatitis-Indiana virus Virus Inhibitors Virus Replication
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v9_n4_p221_Andrei http://hdl.handle.net/20.500.12110/paper_01663542_v9_n4_p221_Andrei
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01663542_v9_n4_p221_Andrei
record_format	dspace
spelling	paper:paper_01663542_v9_n4_p221_Andrei2023-06-08T15:15:43Z Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L Andrei, Graciela Mónica Damonte, Elsa Beatriz Coto, Celia Esther Antiviral state Melia azedarach L Plant antiviral dactinomycin meliacine unclassified drug cell culture chicken drug mechanism nonhuman plant plant leaf priority journal virus infection Animal Antiviral Agents Cells, Cultured Human Interferons Kinetics Plants Support, Non-U.S. Gov't Vesicular stomatitis-Indiana virus Virus Inhibitors Virus Replication A partially purified plant inhibitor (Meliacin) isolated from Melia azedarach L induced in cells a refractory state to virus infection. Meliacin was active in a large variety of continuous and/or primary cell cultures. A state of maximum virus resistance was achieved after 2 h of incubation and was maintained for at least 15 h; later on it declined but it was fully regained after a second pulse of Meliacin. Interferon was not detected in the supernatant of cells treated with Meliacin and a measurable increase in ds-RNA dependent protein kinase activity was not observed in extracts of Meliacin-treated cells. The antiviral state was not transferred by either extracellular fluid or direct cell-to-cell contact. An active cell metabolism was required for Meliacin action, which was partially reversed in the presence of actinomycin D. It appears that Meliacin is not an interferon-like substance, which induces an antiviral state based on a still unexplained mechanism. © 1988. Fil:Andrei, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1988 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v9_n4_p221_Andrei http://hdl.handle.net/20.500.12110/paper_01663542_v9_n4_p221_Andrei
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antiviral state Melia azedarach L Plant antiviral dactinomycin meliacine unclassified drug cell culture chicken drug mechanism nonhuman plant plant leaf priority journal virus infection Animal Antiviral Agents Cells, Cultured Human Interferons Kinetics Plants Support, Non-U.S. Gov't Vesicular stomatitis-Indiana virus Virus Inhibitors Virus Replication
spellingShingle	Antiviral state Melia azedarach L Plant antiviral dactinomycin meliacine unclassified drug cell culture chicken drug mechanism nonhuman plant plant leaf priority journal virus infection Animal Antiviral Agents Cells, Cultured Human Interferons Kinetics Plants Support, Non-U.S. Gov't Vesicular stomatitis-Indiana virus Virus Inhibitors Virus Replication Andrei, Graciela Mónica Damonte, Elsa Beatriz Coto, Celia Esther Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L
topic_facet	Antiviral state Melia azedarach L Plant antiviral dactinomycin meliacine unclassified drug cell culture chicken drug mechanism nonhuman plant plant leaf priority journal virus infection Animal Antiviral Agents Cells, Cultured Human Interferons Kinetics Plants Support, Non-U.S. Gov't Vesicular stomatitis-Indiana virus Virus Inhibitors Virus Replication
description	A partially purified plant inhibitor (Meliacin) isolated from Melia azedarach L induced in cells a refractory state to virus infection. Meliacin was active in a large variety of continuous and/or primary cell cultures. A state of maximum virus resistance was achieved after 2 h of incubation and was maintained for at least 15 h; later on it declined but it was fully regained after a second pulse of Meliacin. Interferon was not detected in the supernatant of cells treated with Meliacin and a measurable increase in ds-RNA dependent protein kinase activity was not observed in extracts of Meliacin-treated cells. The antiviral state was not transferred by either extracellular fluid or direct cell-to-cell contact. An active cell metabolism was required for Meliacin action, which was partially reversed in the presence of actinomycin D. It appears that Meliacin is not an interferon-like substance, which induces an antiviral state based on a still unexplained mechanism. © 1988.
author	Andrei, Graciela Mónica Damonte, Elsa Beatriz Coto, Celia Esther
author_facet	Andrei, Graciela Mónica Damonte, Elsa Beatriz Coto, Celia Esther
author_sort	Andrei, Graciela Mónica
title	Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L
title_short	Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L
title_full	Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L
title_fullStr	Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L
title_full_unstemmed	Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L
title_sort	induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of melia azedarach l
publishDate	1988
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01663542_v9_n4_p221_Andrei http://hdl.handle.net/20.500.12110/paper_01663542_v9_n4_p221_Andrei
work_keys_str_mv	AT andreigracielamonica inductionofarefractorystatetoviralinfectioninmammaliancellsbyaplantinhibitorisolatedfromleavesofmeliaazedarachl AT damonteelsabeatriz inductionofarefractorystatetoviralinfectioninmammaliancellsbyaplantinhibitorisolatedfromleavesofmeliaazedarachl AT cotoceliaesther inductionofarefractorystatetoviralinfectioninmammaliancellsbyaplantinhibitorisolatedfromleavesofmeliaazedarachl
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Induction of a refractory state to viral infection in mammalian cells by a plant inhibitor isolated from leaves of Melia azedarach L

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