Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice
We used β-endorphin-deficient mice as a novel approach to confirm the physiological role that opioid peptides play in the development or regulation of the immune system. We found that mice lacking β-endorphin possessed an enhanced immune response, measured in terms of splenocyte proliferation and in...
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2002
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01655728_v131_n1-2_p126_Refojo http://hdl.handle.net/20.500.12110/paper_01655728_v131_n1-2_p126_Refojo |
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paper:paper_01655728_v131_n1-2_p126_Refojo2023-06-08T15:14:54Z Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice Refojo, Damián Kovalovsky, Damián Young, Juan I. Rubinstein, Marcelo β-endorphin Cytokines HPA axis Neuroimmunology Splenocyte beta endorphin corticosterone corticotropin interleukin 12 interleukin 6 lipopolysaccharide messenger RNA tumor necrosis factor alpha animal cell animal experiment animal model animal tissue article cell proliferation controlled study corticosterone blood level corticotropin blood level cytokine production immune response immune system immunoregulation macrophage mouse nonhuman priority journal protein blood level protein deficiency spleen cell Adrenocorticotropic Hormone Animals beta-Endorphin Cell Division Cells, Cultured Corticosterone Cytokines Interleukin-2 Interleukin-6 Lipopolysaccharides Mice Mice, Inbred C57BL Mice, Knockout RNA, Messenger Spleen Tumor Necrosis Factor-alpha We used β-endorphin-deficient mice as a novel approach to confirm the physiological role that opioid peptides play in the development or regulation of the immune system. We found that mice lacking β-endorphin possessed an enhanced immune response, measured in terms of splenocyte proliferation and interleukin (IL)-2 mRNA levels, in vitro production of the splenic macrophage inflammatory cytokines IL-6 and Tumor Necrosis Factor (TNF)-α and plasma IL-6 following lipopolysaccharide (LPS) administration. β-Endorphin-deficient mice had attenuated increases of plasma ACTH and corticosterone levels in response to LPS. These results are consistent with a postulated inhibitory role of endogenous β-endorphin on the immune system at multiple levels. © 2002 Elsevier Science B.V. All rights reserved. Fil:Refojo, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kovalovsky, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Young, J.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rubinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01655728_v131_n1-2_p126_Refojo http://hdl.handle.net/20.500.12110/paper_01655728_v131_n1-2_p126_Refojo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
β-endorphin Cytokines HPA axis Neuroimmunology Splenocyte beta endorphin corticosterone corticotropin interleukin 12 interleukin 6 lipopolysaccharide messenger RNA tumor necrosis factor alpha animal cell animal experiment animal model animal tissue article cell proliferation controlled study corticosterone blood level corticotropin blood level cytokine production immune response immune system immunoregulation macrophage mouse nonhuman priority journal protein blood level protein deficiency spleen cell Adrenocorticotropic Hormone Animals beta-Endorphin Cell Division Cells, Cultured Corticosterone Cytokines Interleukin-2 Interleukin-6 Lipopolysaccharides Mice Mice, Inbred C57BL Mice, Knockout RNA, Messenger Spleen Tumor Necrosis Factor-alpha |
| spellingShingle |
β-endorphin Cytokines HPA axis Neuroimmunology Splenocyte beta endorphin corticosterone corticotropin interleukin 12 interleukin 6 lipopolysaccharide messenger RNA tumor necrosis factor alpha animal cell animal experiment animal model animal tissue article cell proliferation controlled study corticosterone blood level corticotropin blood level cytokine production immune response immune system immunoregulation macrophage mouse nonhuman priority journal protein blood level protein deficiency spleen cell Adrenocorticotropic Hormone Animals beta-Endorphin Cell Division Cells, Cultured Corticosterone Cytokines Interleukin-2 Interleukin-6 Lipopolysaccharides Mice Mice, Inbred C57BL Mice, Knockout RNA, Messenger Spleen Tumor Necrosis Factor-alpha Refojo, Damián Kovalovsky, Damián Young, Juan I. Rubinstein, Marcelo Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice |
| topic_facet |
β-endorphin Cytokines HPA axis Neuroimmunology Splenocyte beta endorphin corticosterone corticotropin interleukin 12 interleukin 6 lipopolysaccharide messenger RNA tumor necrosis factor alpha animal cell animal experiment animal model animal tissue article cell proliferation controlled study corticosterone blood level corticotropin blood level cytokine production immune response immune system immunoregulation macrophage mouse nonhuman priority journal protein blood level protein deficiency spleen cell Adrenocorticotropic Hormone Animals beta-Endorphin Cell Division Cells, Cultured Corticosterone Cytokines Interleukin-2 Interleukin-6 Lipopolysaccharides Mice Mice, Inbred C57BL Mice, Knockout RNA, Messenger Spleen Tumor Necrosis Factor-alpha |
| description |
We used β-endorphin-deficient mice as a novel approach to confirm the physiological role that opioid peptides play in the development or regulation of the immune system. We found that mice lacking β-endorphin possessed an enhanced immune response, measured in terms of splenocyte proliferation and interleukin (IL)-2 mRNA levels, in vitro production of the splenic macrophage inflammatory cytokines IL-6 and Tumor Necrosis Factor (TNF)-α and plasma IL-6 following lipopolysaccharide (LPS) administration. β-Endorphin-deficient mice had attenuated increases of plasma ACTH and corticosterone levels in response to LPS. These results are consistent with a postulated inhibitory role of endogenous β-endorphin on the immune system at multiple levels. © 2002 Elsevier Science B.V. All rights reserved. |
| author |
Refojo, Damián Kovalovsky, Damián Young, Juan I. Rubinstein, Marcelo |
| author_facet |
Refojo, Damián Kovalovsky, Damián Young, Juan I. Rubinstein, Marcelo |
| author_sort |
Refojo, Damián |
| title |
Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice |
| title_short |
Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice |
| title_full |
Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice |
| title_fullStr |
Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice |
| title_full_unstemmed |
Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice |
| title_sort |
increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice |
| publishDate |
2002 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01655728_v131_n1-2_p126_Refojo http://hdl.handle.net/20.500.12110/paper_01655728_v131_n1-2_p126_Refojo |
| work_keys_str_mv |
AT refojodamian increasedsplenocyteproliferativeresponseandcytokineproductioninbendorphindeficientmice AT kovalovskydamian increasedsplenocyteproliferativeresponseandcytokineproductioninbendorphindeficientmice AT youngjuani increasedsplenocyteproliferativeresponseandcytokineproductioninbendorphindeficientmice AT rubinsteinmarcelo increasedsplenocyteproliferativeresponseandcytokineproductioninbendorphindeficientmice |
| _version_ |
1768545642042359808 |