Nitric oxide modulation of the immune response against cholera toxin-adjuvated ovalbumin administered by the intranasal route
Here, we studied the effect of aminoguanidine (AG) treatment, a nitric oxide synthase (NOS)-2 inhibitor, during the immune response against intranasal administration of ovalbumin (OVA) mixed with cholera toxin (CT) in BALB/c mice. NOS-2 mRNA was detected by reverse transcription-PCR (RT-PCR) in samp...
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2004
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01652478_v92_n3_p245_Gamba http://hdl.handle.net/20.500.12110/paper_01652478_v92_n3_p245_Gamba |
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paper:paper_01652478_v92_n3_p245_Gamba2023-06-08T15:14:52Z Nitric oxide modulation of the immune response against cholera toxin-adjuvated ovalbumin administered by the intranasal route Cholera toxin Mucosal immunity Nitric oxide aminoguanidine cholera toxin immunoglobulin A immunoglobulin G immunoglobulin G1 immunoglobulin G2a immunoglobulin M nitric oxide nitric oxide synthase inhibitor ovalbumin transforming growth factor beta analytic method animal cell antigen specificity article controlled study drug effect drug inhibition immune response inoculation male mouse mouse strain mucosal immunity nonhuman priority journal reverse transcription polymerase chain reaction sampling spleen cell Administration, Intranasal Animals Cholera Toxin Guanidines Immunity, Cellular Immunity, Mucosal Lung Mice Nitric Oxide Nitric Oxide Synthase Ovalbumin Transforming Growth Factor beta Turbinates Here, we studied the effect of aminoguanidine (AG) treatment, a nitric oxide synthase (NOS)-2 inhibitor, during the immune response against intranasal administration of ovalbumin (OVA) mixed with cholera toxin (CT) in BALB/c mice. NOS-2 mRNA was detected by reverse transcription-PCR (RT-PCR) in samples of lungs and turbinates early post-inoculation of the antigen. Animals intranasally treated with AG, showed an increase in the levels of seric specific IgG and IgM. A higher IgG1/IgG2a ratio against OVA was also observed in sera of same animals. Moreover, high levels of specific IgA were detected in samples of pulmonar washings obtained from treated animals. On the contrary, treated animals showed a lower DTH response while splenocytes obtained from the same animals showed a reduced proliferative capability against OVA compared to controls. Finally, RT-PCR analysis showed increased expression of TGF-β in turbinates, lungs and cells from pulmonar washings obtained from AG treated mice. Taken together, these data suggest a role of nitric oxide (NO) in modulating the primary immune response against intranasal antigens. © 2004 Published by Elsevier B.V. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01652478_v92_n3_p245_Gamba http://hdl.handle.net/20.500.12110/paper_01652478_v92_n3_p245_Gamba |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cholera toxin Mucosal immunity Nitric oxide aminoguanidine cholera toxin immunoglobulin A immunoglobulin G immunoglobulin G1 immunoglobulin G2a immunoglobulin M nitric oxide nitric oxide synthase inhibitor ovalbumin transforming growth factor beta analytic method animal cell antigen specificity article controlled study drug effect drug inhibition immune response inoculation male mouse mouse strain mucosal immunity nonhuman priority journal reverse transcription polymerase chain reaction sampling spleen cell Administration, Intranasal Animals Cholera Toxin Guanidines Immunity, Cellular Immunity, Mucosal Lung Mice Nitric Oxide Nitric Oxide Synthase Ovalbumin Transforming Growth Factor beta Turbinates |
| spellingShingle |
Cholera toxin Mucosal immunity Nitric oxide aminoguanidine cholera toxin immunoglobulin A immunoglobulin G immunoglobulin G1 immunoglobulin G2a immunoglobulin M nitric oxide nitric oxide synthase inhibitor ovalbumin transforming growth factor beta analytic method animal cell antigen specificity article controlled study drug effect drug inhibition immune response inoculation male mouse mouse strain mucosal immunity nonhuman priority journal reverse transcription polymerase chain reaction sampling spleen cell Administration, Intranasal Animals Cholera Toxin Guanidines Immunity, Cellular Immunity, Mucosal Lung Mice Nitric Oxide Nitric Oxide Synthase Ovalbumin Transforming Growth Factor beta Turbinates Nitric oxide modulation of the immune response against cholera toxin-adjuvated ovalbumin administered by the intranasal route |
| topic_facet |
Cholera toxin Mucosal immunity Nitric oxide aminoguanidine cholera toxin immunoglobulin A immunoglobulin G immunoglobulin G1 immunoglobulin G2a immunoglobulin M nitric oxide nitric oxide synthase inhibitor ovalbumin transforming growth factor beta analytic method animal cell antigen specificity article controlled study drug effect drug inhibition immune response inoculation male mouse mouse strain mucosal immunity nonhuman priority journal reverse transcription polymerase chain reaction sampling spleen cell Administration, Intranasal Animals Cholera Toxin Guanidines Immunity, Cellular Immunity, Mucosal Lung Mice Nitric Oxide Nitric Oxide Synthase Ovalbumin Transforming Growth Factor beta Turbinates |
| description |
Here, we studied the effect of aminoguanidine (AG) treatment, a nitric oxide synthase (NOS)-2 inhibitor, during the immune response against intranasal administration of ovalbumin (OVA) mixed with cholera toxin (CT) in BALB/c mice. NOS-2 mRNA was detected by reverse transcription-PCR (RT-PCR) in samples of lungs and turbinates early post-inoculation of the antigen. Animals intranasally treated with AG, showed an increase in the levels of seric specific IgG and IgM. A higher IgG1/IgG2a ratio against OVA was also observed in sera of same animals. Moreover, high levels of specific IgA were detected in samples of pulmonar washings obtained from treated animals. On the contrary, treated animals showed a lower DTH response while splenocytes obtained from the same animals showed a reduced proliferative capability against OVA compared to controls. Finally, RT-PCR analysis showed increased expression of TGF-β in turbinates, lungs and cells from pulmonar washings obtained from AG treated mice. Taken together, these data suggest a role of nitric oxide (NO) in modulating the primary immune response against intranasal antigens. © 2004 Published by Elsevier B.V. |
| title |
Nitric oxide modulation of the immune response against cholera toxin-adjuvated ovalbumin administered by the intranasal route |
| title_short |
Nitric oxide modulation of the immune response against cholera toxin-adjuvated ovalbumin administered by the intranasal route |
| title_full |
Nitric oxide modulation of the immune response against cholera toxin-adjuvated ovalbumin administered by the intranasal route |
| title_fullStr |
Nitric oxide modulation of the immune response against cholera toxin-adjuvated ovalbumin administered by the intranasal route |
| title_full_unstemmed |
Nitric oxide modulation of the immune response against cholera toxin-adjuvated ovalbumin administered by the intranasal route |
| title_sort |
nitric oxide modulation of the immune response against cholera toxin-adjuvated ovalbumin administered by the intranasal route |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01652478_v92_n3_p245_Gamba http://hdl.handle.net/20.500.12110/paper_01652478_v92_n3_p245_Gamba |
| _version_ |
1768543555473637376 |