Does hypoxia selectively stimulate the generation of prostaglandin E1 by the isolated rat uterus?
The contractile activity of uterine horns maintained for 90 to 120 minutes under normal oxygenation (carbogen or 100% O2) became undetectable. When in this condition the gassing was stopped one or two minutes later, regular phasic contractions appeared super-imposed on a small increment of the basal...
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1981
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01614630_v7_n5_p375_Gimeno http://hdl.handle.net/20.500.12110/paper_01614630_v7_n5_p375_Gimeno |
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paper:paper_01614630_v7_n5_p375_Gimeno2023-06-08T15:13:28Z Does hypoxia selectively stimulate the generation of prostaglandin E1 by the isolated rat uterus? acetylsalicylic acid imidazole indometacin prostacyclin prostaglandin e1 prostaglandin e2 prostaglandin f2 alpha radioisotope tranylcypromine animal experiment dose response drug response female genital system hypoxia in vitro study radioimmunoassay rat uterus contractility uterus contraction Alprostadil Animals Anoxia Dinoprost Dinoprostone Female Oxygen Prostaglandins E Prostaglandins F Rats Rats, Inbred Strains Uterine Contraction Uterus The contractile activity of uterine horns maintained for 90 to 120 minutes under normal oxygenation (carbogen or 100% O2) became undetectable. When in this condition the gassing was stopped one or two minutes later, regular phasic contractions appeared super-imposed on a small increment of the basal resting tone. Indomethacin and aspirin well known inhibitors of prostaglandin (PG) synthesis, blocked the contractile influence of hypoxia whereas neither tranylcypromine or imidazole were able to alter the stimulatery action. PGE2, PGE1 and PGF2α released into the bathing solution during 10 minutes of normoxia or 10 minutes of hypoxia, were measured. Under O2, PGE2 and PGF2α production diminished significantly (P < 0.05 and P < 0.01, normoxia vs. hypoxia, respectively) whereas PGE1 increased (P < 0.05). "PGI2-like material" generated was also detected and it was found that the values during hypoxia were lower than those observed in 02; however the difference was not statistically significant. Dose-response contractile activity to PGs with and without gassing was explored. It was necessary to add 100 times more PGF2α to obtain the minimal response under hypoxic conditions as compared to normoxia. On the other hand the threshold response to PGE1 was 10 times lower under hypoxic conditions than in normoxia. The possible mechsnism(s) that induce an increment in PGE1 generation accompanied by a simultaneous decrement of PGE2 during hypoxia is discussed in connection with a possible role of PGE1 evoking uterine contractions when the gassing of the suspending solution is stopped. © 1981. 1981 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01614630_v7_n5_p375_Gimeno http://hdl.handle.net/20.500.12110/paper_01614630_v7_n5_p375_Gimeno |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
acetylsalicylic acid imidazole indometacin prostacyclin prostaglandin e1 prostaglandin e2 prostaglandin f2 alpha radioisotope tranylcypromine animal experiment dose response drug response female genital system hypoxia in vitro study radioimmunoassay rat uterus contractility uterus contraction Alprostadil Animals Anoxia Dinoprost Dinoprostone Female Oxygen Prostaglandins E Prostaglandins F Rats Rats, Inbred Strains Uterine Contraction Uterus |
| spellingShingle |
acetylsalicylic acid imidazole indometacin prostacyclin prostaglandin e1 prostaglandin e2 prostaglandin f2 alpha radioisotope tranylcypromine animal experiment dose response drug response female genital system hypoxia in vitro study radioimmunoassay rat uterus contractility uterus contraction Alprostadil Animals Anoxia Dinoprost Dinoprostone Female Oxygen Prostaglandins E Prostaglandins F Rats Rats, Inbred Strains Uterine Contraction Uterus Does hypoxia selectively stimulate the generation of prostaglandin E1 by the isolated rat uterus? |
| topic_facet |
acetylsalicylic acid imidazole indometacin prostacyclin prostaglandin e1 prostaglandin e2 prostaglandin f2 alpha radioisotope tranylcypromine animal experiment dose response drug response female genital system hypoxia in vitro study radioimmunoassay rat uterus contractility uterus contraction Alprostadil Animals Anoxia Dinoprost Dinoprostone Female Oxygen Prostaglandins E Prostaglandins F Rats Rats, Inbred Strains Uterine Contraction Uterus |
| description |
The contractile activity of uterine horns maintained for 90 to 120 minutes under normal oxygenation (carbogen or 100% O2) became undetectable. When in this condition the gassing was stopped one or two minutes later, regular phasic contractions appeared super-imposed on a small increment of the basal resting tone. Indomethacin and aspirin well known inhibitors of prostaglandin (PG) synthesis, blocked the contractile influence of hypoxia whereas neither tranylcypromine or imidazole were able to alter the stimulatery action. PGE2, PGE1 and PGF2α released into the bathing solution during 10 minutes of normoxia or 10 minutes of hypoxia, were measured. Under O2, PGE2 and PGF2α production diminished significantly (P < 0.05 and P < 0.01, normoxia vs. hypoxia, respectively) whereas PGE1 increased (P < 0.05). "PGI2-like material" generated was also detected and it was found that the values during hypoxia were lower than those observed in 02; however the difference was not statistically significant. Dose-response contractile activity to PGs with and without gassing was explored. It was necessary to add 100 times more PGF2α to obtain the minimal response under hypoxic conditions as compared to normoxia. On the other hand the threshold response to PGE1 was 10 times lower under hypoxic conditions than in normoxia. The possible mechsnism(s) that induce an increment in PGE1 generation accompanied by a simultaneous decrement of PGE2 during hypoxia is discussed in connection with a possible role of PGE1 evoking uterine contractions when the gassing of the suspending solution is stopped. © 1981. |
| title |
Does hypoxia selectively stimulate the generation of prostaglandin E1 by the isolated rat uterus? |
| title_short |
Does hypoxia selectively stimulate the generation of prostaglandin E1 by the isolated rat uterus? |
| title_full |
Does hypoxia selectively stimulate the generation of prostaglandin E1 by the isolated rat uterus? |
| title_fullStr |
Does hypoxia selectively stimulate the generation of prostaglandin E1 by the isolated rat uterus? |
| title_full_unstemmed |
Does hypoxia selectively stimulate the generation of prostaglandin E1 by the isolated rat uterus? |
| title_sort |
does hypoxia selectively stimulate the generation of prostaglandin e1 by the isolated rat uterus? |
| publishDate |
1981 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01614630_v7_n5_p375_Gimeno http://hdl.handle.net/20.500.12110/paper_01614630_v7_n5_p375_Gimeno |
| _version_ |
1768546670887305216 |