Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes
Trypomastigotes were metabolically labeled with [3H]-palmitic acid or [3H] -galactose and labeled components were detected in the culture medium. Thin layer chromatography of the shed material showed several lipids in the [3H] -palmitic acid labeled sample while the sugar was mainly incorporated int...
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1991
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01585231_v24_n6_p991_Couto http://hdl.handle.net/20.500.12110/paper_01585231_v24_n6_p991_Couto |
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paper:paper_01585231_v24_n6_p991_Couto |
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paper:paper_01585231_v24_n6_p991_Couto2023-06-08T15:13:24Z Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes endogenous compound galactose glycoconjugate lipid membrane lipid palmitic acid parasite antigen galactose glycoconjugate lipid palmitic acid derivative article cell culture cell surface culture medium macromolecule membrane vesicle nonhuman ph priority journal thin layer chromatography trypanosoma cruzi trypomastigote animal chemistry culture medium fractionation gel chromatography metabolism Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Animal Chemical Fractionation Chromatography, Gel Chromatography, Thin Layer Culture Media Galactose Glycoconjugates Lipids Palmitic Acid Palmitic Acids Support, Non-U.S. Gov't Trypanosoma cruzi Trypomastigotes were metabolically labeled with [3H]-palmitic acid or [3H] -galactose and labeled components were detected in the culture medium. Thin layer chromatography of the shed material showed several lipids in the [3H] -palmitic acid labeled sample while the sugar was mainly incorporated into macromolecules. The material incorporated with the lipidic precursor was fractionated by DEAE-Sephadex (acetate form) and the amount of radioactivity was ten times higher in the acidic lipids than in the neutral lipids. When acidic lipids were further separated by Unisil, 73% of the radioactivity was recovered in the less polar fraction. Different patterns were obtained on comparison of the shed components with the lipids remaining in the parasite. 1991 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01585231_v24_n6_p991_Couto http://hdl.handle.net/20.500.12110/paper_01585231_v24_n6_p991_Couto |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
endogenous compound galactose glycoconjugate lipid membrane lipid palmitic acid parasite antigen galactose glycoconjugate lipid palmitic acid derivative article cell culture cell surface culture medium macromolecule membrane vesicle nonhuman ph priority journal thin layer chromatography trypanosoma cruzi trypomastigote animal chemistry culture medium fractionation gel chromatography metabolism Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Animal Chemical Fractionation Chromatography, Gel Chromatography, Thin Layer Culture Media Galactose Glycoconjugates Lipids Palmitic Acid Palmitic Acids Support, Non-U.S. Gov't Trypanosoma cruzi |
| spellingShingle |
endogenous compound galactose glycoconjugate lipid membrane lipid palmitic acid parasite antigen galactose glycoconjugate lipid palmitic acid derivative article cell culture cell surface culture medium macromolecule membrane vesicle nonhuman ph priority journal thin layer chromatography trypanosoma cruzi trypomastigote animal chemistry culture medium fractionation gel chromatography metabolism Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Animal Chemical Fractionation Chromatography, Gel Chromatography, Thin Layer Culture Media Galactose Glycoconjugates Lipids Palmitic Acid Palmitic Acids Support, Non-U.S. Gov't Trypanosoma cruzi Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes |
| topic_facet |
endogenous compound galactose glycoconjugate lipid membrane lipid palmitic acid parasite antigen galactose glycoconjugate lipid palmitic acid derivative article cell culture cell surface culture medium macromolecule membrane vesicle nonhuman ph priority journal thin layer chromatography trypanosoma cruzi trypomastigote animal chemistry culture medium fractionation gel chromatography metabolism Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Animal Chemical Fractionation Chromatography, Gel Chromatography, Thin Layer Culture Media Galactose Glycoconjugates Lipids Palmitic Acid Palmitic Acids Support, Non-U.S. Gov't Trypanosoma cruzi |
| description |
Trypomastigotes were metabolically labeled with [3H]-palmitic acid or [3H] -galactose and labeled components were detected in the culture medium. Thin layer chromatography of the shed material showed several lipids in the [3H] -palmitic acid labeled sample while the sugar was mainly incorporated into macromolecules. The material incorporated with the lipidic precursor was fractionated by DEAE-Sephadex (acetate form) and the amount of radioactivity was ten times higher in the acidic lipids than in the neutral lipids. When acidic lipids were further separated by Unisil, 73% of the radioactivity was recovered in the less polar fraction. Different patterns were obtained on comparison of the shed components with the lipids remaining in the parasite. |
| title |
Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes |
| title_short |
Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes |
| title_full |
Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes |
| title_fullStr |
Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes |
| title_full_unstemmed |
Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes |
| title_sort |
trypanosoma cruzi's incorporation of [3h]-palmitic acid and [3h] -galactose into components shed by trypomastigotes |
| publishDate |
1991 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01585231_v24_n6_p991_Couto http://hdl.handle.net/20.500.12110/paper_01585231_v24_n6_p991_Couto |
| _version_ |
1768543272461926400 |