Kinetic and molecular parameters of human hepatic porphobilinogen deaminase
A basal level of human liver porphobilinogen deaminase of 3.66 units/g wet weight was found in adult tissue. Activity in neonatal liver was at least three fold higher. Physico-chemical studies revealed that the enzyme has the approximate form of a symmetrical molecule and exhibits hyperbolic kinetic...
| Autores principales: | , |
|---|---|
| Publicado: |
1990
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01585231_v21_n3_p463_Mazzetti http://hdl.handle.net/20.500.12110/paper_01585231_v21_n3_p463_Mazzetti |
| Aporte de: |
| id |
paper:paper_01585231_v21_n3_p463_Mazzetti |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_01585231_v21_n3_p463_Mazzetti2023-06-08T15:13:24Z Kinetic and molecular parameters of human hepatic porphobilinogen deaminase Mazzetti, Marta Blanca Tomio, Josefina María liver enzyme porphobilinogen deaminase article human human cell priority journal Adult Human Hydrogen-Ion Concentration Hydroxymethylbilane Synthase Infant, Newborn Isoelectric Focusing Kinetics Liver Support, Non-U.S. Gov't A basal level of human liver porphobilinogen deaminase of 3.66 units/g wet weight was found in adult tissue. Activity in neonatal liver was at least three fold higher. Physico-chemical studies revealed that the enzyme has the approximate form of a symmetrical molecule and exhibits hyperbolic kinetics with a Km value of 3.6 μM at pH 7.6. Two ionizable groups with pK values of 7.35 and 8.90 are prominent for catalysis. A set of pI (5.8 - 4.9) were observed under different conditions. Results demonstrate the existence of a single protein differentially charged with multiple molecular forms in adult liver. Fil:Mazzetti, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tomio, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1990 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01585231_v21_n3_p463_Mazzetti http://hdl.handle.net/20.500.12110/paper_01585231_v21_n3_p463_Mazzetti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
liver enzyme porphobilinogen deaminase article human human cell priority journal Adult Human Hydrogen-Ion Concentration Hydroxymethylbilane Synthase Infant, Newborn Isoelectric Focusing Kinetics Liver Support, Non-U.S. Gov't |
| spellingShingle |
liver enzyme porphobilinogen deaminase article human human cell priority journal Adult Human Hydrogen-Ion Concentration Hydroxymethylbilane Synthase Infant, Newborn Isoelectric Focusing Kinetics Liver Support, Non-U.S. Gov't Mazzetti, Marta Blanca Tomio, Josefina María Kinetic and molecular parameters of human hepatic porphobilinogen deaminase |
| topic_facet |
liver enzyme porphobilinogen deaminase article human human cell priority journal Adult Human Hydrogen-Ion Concentration Hydroxymethylbilane Synthase Infant, Newborn Isoelectric Focusing Kinetics Liver Support, Non-U.S. Gov't |
| description |
A basal level of human liver porphobilinogen deaminase of 3.66 units/g wet weight was found in adult tissue. Activity in neonatal liver was at least three fold higher. Physico-chemical studies revealed that the enzyme has the approximate form of a symmetrical molecule and exhibits hyperbolic kinetics with a Km value of 3.6 μM at pH 7.6. Two ionizable groups with pK values of 7.35 and 8.90 are prominent for catalysis. A set of pI (5.8 - 4.9) were observed under different conditions. Results demonstrate the existence of a single protein differentially charged with multiple molecular forms in adult liver. |
| author |
Mazzetti, Marta Blanca Tomio, Josefina María |
| author_facet |
Mazzetti, Marta Blanca Tomio, Josefina María |
| author_sort |
Mazzetti, Marta Blanca |
| title |
Kinetic and molecular parameters of human hepatic porphobilinogen deaminase |
| title_short |
Kinetic and molecular parameters of human hepatic porphobilinogen deaminase |
| title_full |
Kinetic and molecular parameters of human hepatic porphobilinogen deaminase |
| title_fullStr |
Kinetic and molecular parameters of human hepatic porphobilinogen deaminase |
| title_full_unstemmed |
Kinetic and molecular parameters of human hepatic porphobilinogen deaminase |
| title_sort |
kinetic and molecular parameters of human hepatic porphobilinogen deaminase |
| publishDate |
1990 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01585231_v21_n3_p463_Mazzetti http://hdl.handle.net/20.500.12110/paper_01585231_v21_n3_p463_Mazzetti |
| work_keys_str_mv |
AT mazzettimartablanca kineticandmolecularparametersofhumanhepaticporphobilinogendeaminase AT tomiojosefinamaria kineticandmolecularparametersofhumanhepaticporphobilinogendeaminase |
| _version_ |
1768542215544504320 |