Emergence of herpes simplex virus-1 syncytial variants with altered virulence for mice after selection with a natural carrageenan
Background: Antiviral therapy against herpes simplex virus based on sulfated polysaccharides, like carrageenans, represents a new alternative for genital herpes infections treatment and arises the concern about the appearance of resistant viral populations. Methods: We characterized the F strain of...
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2011
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01485717_v38_n6_p555_Mateu http://hdl.handle.net/20.500.12110/paper_01485717_v38_n6_p555_Mateu |
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paper:paper_01485717_v38_n6_p555_Mateu2023-06-08T15:13:11Z Emergence of herpes simplex virus-1 syncytial variants with altered virulence for mice after selection with a natural carrageenan Mateu, Cecilia Gabriela Pérez Recalde, María Mercedes Artuso, María Carolina Hermida, Gladys N. Linero, Florencia N. Scolaro, Luis Alberto Damonte, Elsa Beatriz Pujol, Carlos Alberto Carlucci, María Josefina 5 (2 bromovinyl) 2' deoxyuridine aciclovir carrageenan gamma interferon heparin interleukin 10 interleukin 1beta interleukin 6 tumor necrosis factor alpha animal tissue antiviral susceptibility article Bagg albino mouse controlled study female Gigartina skottbergii Herpes simplex virus 1 IC 50 in vitro study mouse nonhuman phenotype seaweed vaginal lavage virus attenuation virus isolation virus resistance virus strain virus virulence Animals Antiviral Agents Carrageenan Cercopithecus aethiops Female Genetic Variation Giant Cells Herpes Genitalis Herpes Simplex Herpesvirus 1, Human Mice Mice, Inbred BALB C Mice, Inbred C57BL Microbial Sensitivity Tests Rhodophyta Seaweed Selection, Genetic Vero Cells Virulence Background: Antiviral therapy against herpes simplex virus based on sulfated polysaccharides, like carrageenans, represents a new alternative for genital herpes infections treatment and arises the concern about the appearance of resistant viral populations. Methods: We characterized the F strain of herpes simplex virus-1 passaged in the presence of a natural carrageenan isolated from the red seaweed Gigartina skottbergii in view of the virulence for mice of isolated viral clones. Results: Viral clones (syn14-1 and syn17-2) showed a syncytial phenotype and a mild resistance to carrageenan, heparin, acyclovir, and brivudine. Both clones were avirulent for BALB/c mice when inoculated intravaginally, whereas F strain produced high mortality. Attenuation correlated with low levels of TNF-α, interleukin-6, and IFN-γ in vaginal lavages although virus titers were similar to those obtained for F strain. On the contrary, they showed a marked virulence when inoculated intranasally leading to a generalized spreading of virus. Conclusions: Results confirm the hypothesis that selection of herpes simplex virus-1 with a carrageenan in vitro leads to the emergence of variants with a differential virulence when compared to the original virus. This finding should be addressed when an antiviral therapy against genital herpes infection employing a natural carrageenan is under consideration. © 2011 American Sexually Transmitted Diseases Association All rights reserved. Fil:Mateu, C.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Recalde, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Artuso, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hermida, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Linero, F.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scolaro, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pujol, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carlucci, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01485717_v38_n6_p555_Mateu http://hdl.handle.net/20.500.12110/paper_01485717_v38_n6_p555_Mateu |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
5 (2 bromovinyl) 2' deoxyuridine aciclovir carrageenan gamma interferon heparin interleukin 10 interleukin 1beta interleukin 6 tumor necrosis factor alpha animal tissue antiviral susceptibility article Bagg albino mouse controlled study female Gigartina skottbergii Herpes simplex virus 1 IC 50 in vitro study mouse nonhuman phenotype seaweed vaginal lavage virus attenuation virus isolation virus resistance virus strain virus virulence Animals Antiviral Agents Carrageenan Cercopithecus aethiops Female Genetic Variation Giant Cells Herpes Genitalis Herpes Simplex Herpesvirus 1, Human Mice Mice, Inbred BALB C Mice, Inbred C57BL Microbial Sensitivity Tests Rhodophyta Seaweed Selection, Genetic Vero Cells Virulence |
| spellingShingle |
5 (2 bromovinyl) 2' deoxyuridine aciclovir carrageenan gamma interferon heparin interleukin 10 interleukin 1beta interleukin 6 tumor necrosis factor alpha animal tissue antiviral susceptibility article Bagg albino mouse controlled study female Gigartina skottbergii Herpes simplex virus 1 IC 50 in vitro study mouse nonhuman phenotype seaweed vaginal lavage virus attenuation virus isolation virus resistance virus strain virus virulence Animals Antiviral Agents Carrageenan Cercopithecus aethiops Female Genetic Variation Giant Cells Herpes Genitalis Herpes Simplex Herpesvirus 1, Human Mice Mice, Inbred BALB C Mice, Inbred C57BL Microbial Sensitivity Tests Rhodophyta Seaweed Selection, Genetic Vero Cells Virulence Mateu, Cecilia Gabriela Pérez Recalde, María Mercedes Artuso, María Carolina Hermida, Gladys N. Linero, Florencia N. Scolaro, Luis Alberto Damonte, Elsa Beatriz Pujol, Carlos Alberto Carlucci, María Josefina Emergence of herpes simplex virus-1 syncytial variants with altered virulence for mice after selection with a natural carrageenan |
| topic_facet |
5 (2 bromovinyl) 2' deoxyuridine aciclovir carrageenan gamma interferon heparin interleukin 10 interleukin 1beta interleukin 6 tumor necrosis factor alpha animal tissue antiviral susceptibility article Bagg albino mouse controlled study female Gigartina skottbergii Herpes simplex virus 1 IC 50 in vitro study mouse nonhuman phenotype seaweed vaginal lavage virus attenuation virus isolation virus resistance virus strain virus virulence Animals Antiviral Agents Carrageenan Cercopithecus aethiops Female Genetic Variation Giant Cells Herpes Genitalis Herpes Simplex Herpesvirus 1, Human Mice Mice, Inbred BALB C Mice, Inbred C57BL Microbial Sensitivity Tests Rhodophyta Seaweed Selection, Genetic Vero Cells Virulence |
| description |
Background: Antiviral therapy against herpes simplex virus based on sulfated polysaccharides, like carrageenans, represents a new alternative for genital herpes infections treatment and arises the concern about the appearance of resistant viral populations. Methods: We characterized the F strain of herpes simplex virus-1 passaged in the presence of a natural carrageenan isolated from the red seaweed Gigartina skottbergii in view of the virulence for mice of isolated viral clones. Results: Viral clones (syn14-1 and syn17-2) showed a syncytial phenotype and a mild resistance to carrageenan, heparin, acyclovir, and brivudine. Both clones were avirulent for BALB/c mice when inoculated intravaginally, whereas F strain produced high mortality. Attenuation correlated with low levels of TNF-α, interleukin-6, and IFN-γ in vaginal lavages although virus titers were similar to those obtained for F strain. On the contrary, they showed a marked virulence when inoculated intranasally leading to a generalized spreading of virus. Conclusions: Results confirm the hypothesis that selection of herpes simplex virus-1 with a carrageenan in vitro leads to the emergence of variants with a differential virulence when compared to the original virus. This finding should be addressed when an antiviral therapy against genital herpes infection employing a natural carrageenan is under consideration. © 2011 American Sexually Transmitted Diseases Association All rights reserved. |
| author |
Mateu, Cecilia Gabriela Pérez Recalde, María Mercedes Artuso, María Carolina Hermida, Gladys N. Linero, Florencia N. Scolaro, Luis Alberto Damonte, Elsa Beatriz Pujol, Carlos Alberto Carlucci, María Josefina |
| author_facet |
Mateu, Cecilia Gabriela Pérez Recalde, María Mercedes Artuso, María Carolina Hermida, Gladys N. Linero, Florencia N. Scolaro, Luis Alberto Damonte, Elsa Beatriz Pujol, Carlos Alberto Carlucci, María Josefina |
| author_sort |
Mateu, Cecilia Gabriela |
| title |
Emergence of herpes simplex virus-1 syncytial variants with altered virulence for mice after selection with a natural carrageenan |
| title_short |
Emergence of herpes simplex virus-1 syncytial variants with altered virulence for mice after selection with a natural carrageenan |
| title_full |
Emergence of herpes simplex virus-1 syncytial variants with altered virulence for mice after selection with a natural carrageenan |
| title_fullStr |
Emergence of herpes simplex virus-1 syncytial variants with altered virulence for mice after selection with a natural carrageenan |
| title_full_unstemmed |
Emergence of herpes simplex virus-1 syncytial variants with altered virulence for mice after selection with a natural carrageenan |
| title_sort |
emergence of herpes simplex virus-1 syncytial variants with altered virulence for mice after selection with a natural carrageenan |
| publishDate |
2011 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01485717_v38_n6_p555_Mateu http://hdl.handle.net/20.500.12110/paper_01485717_v38_n6_p555_Mateu |
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