Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri

We studied the toxicity of a glyphosate formulation and provide evidence of metabolic alterations due to oxidative stress caused in a Chlorella kessleri tolerant strain by exposure to the herbicide. After 96h of exposure to increasing concentrations of the herbicide (0-70mgL-1) with alkylaryl polygl...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Romero, Delfina M., Ríos de Molina, María del Carmen, Juárez, Angela Beatriz
Publicado:	2011
Materias:	Antioxidant defences Chlorella kessleri Glyphosate Microalgae Oxidative stress algal protein biological marker catalase glutathione glyphosate malonaldehyde superoxide dismutase surfactant biomarker concentration (composition) ecotoxicology enzyme activity free radical green alga inhibition microalga oxidation pollution exposure algal growth antioxidant activity article biomass cell size cell structure cell vacuole Chlorella chlorophyll content controlled study cytotoxicity test growth inhibition growth rate herbicide tolerance lipid peroxidation nonhuman oxidative stress plant cell plant metabolism protein content sensitivity analysis sporangium Biological Markers Catalase Glutathione Glutathione Peroxidase Glutathione Reductase Glycine Herbicides Lipid Peroxidation Malondialdehyde Oxidative Stress Superoxide Dismutase Thiobarbituric Acid Reactive Substances Water Pollutants, Chemical Parachlorella kessleri
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v74_n4_p741_Romero http://hdl.handle.net/20.500.12110/paper_01476513_v74_n4_p741_Romero
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01476513_v74_n4_p741_Romero
record_format	dspace
spelling	paper:paper_01476513_v74_n4_p741_Romero2023-06-08T15:12:58Z Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri Romero, Delfina M. Ríos de Molina, María del Carmen Juárez, Angela Beatriz Antioxidant defences Chlorella kessleri Glyphosate Microalgae Oxidative stress algal protein biological marker catalase glutathione glyphosate malonaldehyde superoxide dismutase surfactant biomarker concentration (composition) ecotoxicology enzyme activity free radical glyphosate green alga inhibition microalga oxidation pollution exposure surfactant algal growth antioxidant activity article biomass cell size cell structure cell vacuole Chlorella Chlorella kessleri chlorophyll content controlled study cytotoxicity test enzyme activity growth inhibition growth rate herbicide tolerance lipid peroxidation nonhuman oxidative stress plant cell plant metabolism protein content sensitivity analysis sporangium Biological Markers Catalase Chlorella Glutathione Glutathione Peroxidase Glutathione Reductase Glycine Herbicides Lipid Peroxidation Malondialdehyde Oxidative Stress Superoxide Dismutase Thiobarbituric Acid Reactive Substances Water Pollutants, Chemical Parachlorella kessleri We studied the toxicity of a glyphosate formulation and provide evidence of metabolic alterations due to oxidative stress caused in a Chlorella kessleri tolerant strain by exposure to the herbicide. After 96h of exposure to increasing concentrations of the herbicide (0-70mgL-1) with alkylaryl polyglycol ether surfactant, growth was inhibited (EC50-96h 55.62mgL-1). Glyphosate increased protein and malondialdehyde content which was significantly higher than in the control at 50-70mgL-1. Superoxide dismutase and catalase activities and reduced glutathione levels increased in a concentration-dependant manner. Morphological studies showed increases in vacuolisation and in cell and sporangia sizes. The glyphosate formulation studied has a cytotoxic effect on C. kessleri through a mechanism that would involve the induction of oxidative stress. Upon glyphosate exposure, oxidative stress parameters such as SOD and CAT activities and MDA level could be more sensitive biomarkers than usually tested growth parameters in C. kessleri. © 2010 Elsevier Inc. Fil:Romero, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ríos de Molina, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Juárez, Á.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v74_n4_p741_Romero http://hdl.handle.net/20.500.12110/paper_01476513_v74_n4_p741_Romero
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antioxidant defences Chlorella kessleri Glyphosate Microalgae Oxidative stress algal protein biological marker catalase glutathione glyphosate malonaldehyde superoxide dismutase surfactant biomarker concentration (composition) ecotoxicology enzyme activity free radical glyphosate green alga inhibition microalga oxidation pollution exposure surfactant algal growth antioxidant activity article biomass cell size cell structure cell vacuole Chlorella Chlorella kessleri chlorophyll content controlled study cytotoxicity test enzyme activity growth inhibition growth rate herbicide tolerance lipid peroxidation nonhuman oxidative stress plant cell plant metabolism protein content sensitivity analysis sporangium Biological Markers Catalase Chlorella Glutathione Glutathione Peroxidase Glutathione Reductase Glycine Herbicides Lipid Peroxidation Malondialdehyde Oxidative Stress Superoxide Dismutase Thiobarbituric Acid Reactive Substances Water Pollutants, Chemical Parachlorella kessleri
spellingShingle	Antioxidant defences Chlorella kessleri Glyphosate Microalgae Oxidative stress algal protein biological marker catalase glutathione glyphosate malonaldehyde superoxide dismutase surfactant biomarker concentration (composition) ecotoxicology enzyme activity free radical glyphosate green alga inhibition microalga oxidation pollution exposure surfactant algal growth antioxidant activity article biomass cell size cell structure cell vacuole Chlorella Chlorella kessleri chlorophyll content controlled study cytotoxicity test enzyme activity growth inhibition growth rate herbicide tolerance lipid peroxidation nonhuman oxidative stress plant cell plant metabolism protein content sensitivity analysis sporangium Biological Markers Catalase Chlorella Glutathione Glutathione Peroxidase Glutathione Reductase Glycine Herbicides Lipid Peroxidation Malondialdehyde Oxidative Stress Superoxide Dismutase Thiobarbituric Acid Reactive Substances Water Pollutants, Chemical Parachlorella kessleri Romero, Delfina M. Ríos de Molina, María del Carmen Juárez, Angela Beatriz Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri
topic_facet	Antioxidant defences Chlorella kessleri Glyphosate Microalgae Oxidative stress algal protein biological marker catalase glutathione glyphosate malonaldehyde superoxide dismutase surfactant biomarker concentration (composition) ecotoxicology enzyme activity free radical glyphosate green alga inhibition microalga oxidation pollution exposure surfactant algal growth antioxidant activity article biomass cell size cell structure cell vacuole Chlorella Chlorella kessleri chlorophyll content controlled study cytotoxicity test enzyme activity growth inhibition growth rate herbicide tolerance lipid peroxidation nonhuman oxidative stress plant cell plant metabolism protein content sensitivity analysis sporangium Biological Markers Catalase Chlorella Glutathione Glutathione Peroxidase Glutathione Reductase Glycine Herbicides Lipid Peroxidation Malondialdehyde Oxidative Stress Superoxide Dismutase Thiobarbituric Acid Reactive Substances Water Pollutants, Chemical Parachlorella kessleri
description	We studied the toxicity of a glyphosate formulation and provide evidence of metabolic alterations due to oxidative stress caused in a Chlorella kessleri tolerant strain by exposure to the herbicide. After 96h of exposure to increasing concentrations of the herbicide (0-70mgL-1) with alkylaryl polyglycol ether surfactant, growth was inhibited (EC50-96h 55.62mgL-1). Glyphosate increased protein and malondialdehyde content which was significantly higher than in the control at 50-70mgL-1. Superoxide dismutase and catalase activities and reduced glutathione levels increased in a concentration-dependant manner. Morphological studies showed increases in vacuolisation and in cell and sporangia sizes. The glyphosate formulation studied has a cytotoxic effect on C. kessleri through a mechanism that would involve the induction of oxidative stress. Upon glyphosate exposure, oxidative stress parameters such as SOD and CAT activities and MDA level could be more sensitive biomarkers than usually tested growth parameters in C. kessleri. © 2010 Elsevier Inc.
author	Romero, Delfina M. Ríos de Molina, María del Carmen Juárez, Angela Beatriz
author_facet	Romero, Delfina M. Ríos de Molina, María del Carmen Juárez, Angela Beatriz
author_sort	Romero, Delfina M.
title	Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri
title_short	Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri
title_full	Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri
title_fullStr	Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri
title_full_unstemmed	Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri
title_sort	oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of chlorella kessleri
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v74_n4_p741_Romero http://hdl.handle.net/20.500.12110/paper_01476513_v74_n4_p741_Romero
work_keys_str_mv	AT romerodelfinam oxidativestressinducedbyacommercialglyphosateformulationinatolerantstrainofchlorellakessleri AT riosdemolinamariadelcarmen oxidativestressinducedbyacommercialglyphosateformulationinatolerantstrainofchlorellakessleri AT juarezangelabeatriz oxidativestressinducedbyacommercialglyphosateformulationinatolerantstrainofchlorellakessleri
_version_	1768546015160303616

Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri

Ejemplares similares