Heme oxygenase, aminolevulinate acid synthetase and the antioxidant system in the brain of mice treated with porphyrinogenic drugs
Several drugs and stress are involved in the triggering of attacks in acute porphyrias. The central nervous system is extremely sensitive to free radical damage because of a relatively low antioxidant capacity. We have demonstrated that mice brain cholinergic system was altered by the effect of some...
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2005
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v51_n5_p487_Rodriguez http://hdl.handle.net/20.500.12110/paper_01455680_v51_n5_p487_Rodriguez |
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paper:paper_01455680_v51_n5_p487_Rodriguez2023-06-08T15:12:26Z Heme oxygenase, aminolevulinate acid synthetase and the antioxidant system in the brain of mice treated with porphyrinogenic drugs Brain Catalase Delta-aminolevulinic acid synthetase Glutathione Glutathione peroxidase Glutathione reductase Heme oxygenase Porphyrinogenic drugs Superoxide dismutase TBARS 5 aminolevulinate synthase acetamide derivative alcohol anesthetic agent barbital catalase enflurane glutathione glutathione peroxidase glutathione reductase heme oxygenase isoflurane messenger RNA porphyrin derivative superoxide dismutase xenobiotic agent animal experiment animal model antioxidant activity article controlled study enzyme activity gene expression mouse neuropathy nonhuman oxidative stress porphyria 5-Aminolevulinate Synthetase Animals Antioxidants Barbital Brain Enflurane Ethanol Gene Expression Regulation, Enzymologic Griseofulvin Heme Oxygenase (Decyclizing) Isoflurane Male Mice Mice, Inbred Strains Oxidative Stress Porphyria, Acute Intermittent Porphyrinogens RNA, Messenger Animalia Several drugs and stress are involved in the triggering of attacks in acute porphyrias. The central nervous system is extremely sensitive to free radical damage because of a relatively low antioxidant capacity. We have demonstrated that mice brain cholinergic system was altered by the effect of some porphyrinogenic agents. The aim of this work was to investigate how known porphyrinogenic drugs affect delta-Aminolevulinic acid synthetase (ALA-S), which is the response of heme oxygenase (HO) to this challenge and to evaluate if the xenobiotics studied develop stress oxidative in mice brain. HO activity was 50-70% induced after chronic Enflurane and Isoflurane anaesthesia, dietary Griseofulvin and starvation. An increase in mRNA HO expression was caused by chronic anaesthesia and Veronal treatments; instead allylisopropilacetamide (AIA) reduced mRNA expression. ALA-S activity was induced by acute administration of anaesthetics (89%), veronal (240%) and ethanol (80%), while ALA-S mRNA expression augmented by chronic administration of enflurane, AIA and veronal. Stress markers such as superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase activities and malondialdehyde and reduced glutathione levels showed different responses depending on the xenobiótic assayed. In conclusion, some of the drugs studied produced oxidative stress in brain that was confirmed through HO induction and this could be one of the factors leading to porphyric neuropathy. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v51_n5_p487_Rodriguez http://hdl.handle.net/20.500.12110/paper_01455680_v51_n5_p487_Rodriguez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Brain Catalase Delta-aminolevulinic acid synthetase Glutathione Glutathione peroxidase Glutathione reductase Heme oxygenase Porphyrinogenic drugs Superoxide dismutase TBARS 5 aminolevulinate synthase acetamide derivative alcohol anesthetic agent barbital catalase enflurane glutathione glutathione peroxidase glutathione reductase heme oxygenase isoflurane messenger RNA porphyrin derivative superoxide dismutase xenobiotic agent animal experiment animal model antioxidant activity article controlled study enzyme activity gene expression mouse neuropathy nonhuman oxidative stress porphyria 5-Aminolevulinate Synthetase Animals Antioxidants Barbital Brain Enflurane Ethanol Gene Expression Regulation, Enzymologic Griseofulvin Heme Oxygenase (Decyclizing) Isoflurane Male Mice Mice, Inbred Strains Oxidative Stress Porphyria, Acute Intermittent Porphyrinogens RNA, Messenger Animalia |
| spellingShingle |
Brain Catalase Delta-aminolevulinic acid synthetase Glutathione Glutathione peroxidase Glutathione reductase Heme oxygenase Porphyrinogenic drugs Superoxide dismutase TBARS 5 aminolevulinate synthase acetamide derivative alcohol anesthetic agent barbital catalase enflurane glutathione glutathione peroxidase glutathione reductase heme oxygenase isoflurane messenger RNA porphyrin derivative superoxide dismutase xenobiotic agent animal experiment animal model antioxidant activity article controlled study enzyme activity gene expression mouse neuropathy nonhuman oxidative stress porphyria 5-Aminolevulinate Synthetase Animals Antioxidants Barbital Brain Enflurane Ethanol Gene Expression Regulation, Enzymologic Griseofulvin Heme Oxygenase (Decyclizing) Isoflurane Male Mice Mice, Inbred Strains Oxidative Stress Porphyria, Acute Intermittent Porphyrinogens RNA, Messenger Animalia Heme oxygenase, aminolevulinate acid synthetase and the antioxidant system in the brain of mice treated with porphyrinogenic drugs |
| topic_facet |
Brain Catalase Delta-aminolevulinic acid synthetase Glutathione Glutathione peroxidase Glutathione reductase Heme oxygenase Porphyrinogenic drugs Superoxide dismutase TBARS 5 aminolevulinate synthase acetamide derivative alcohol anesthetic agent barbital catalase enflurane glutathione glutathione peroxidase glutathione reductase heme oxygenase isoflurane messenger RNA porphyrin derivative superoxide dismutase xenobiotic agent animal experiment animal model antioxidant activity article controlled study enzyme activity gene expression mouse neuropathy nonhuman oxidative stress porphyria 5-Aminolevulinate Synthetase Animals Antioxidants Barbital Brain Enflurane Ethanol Gene Expression Regulation, Enzymologic Griseofulvin Heme Oxygenase (Decyclizing) Isoflurane Male Mice Mice, Inbred Strains Oxidative Stress Porphyria, Acute Intermittent Porphyrinogens RNA, Messenger Animalia |
| description |
Several drugs and stress are involved in the triggering of attacks in acute porphyrias. The central nervous system is extremely sensitive to free radical damage because of a relatively low antioxidant capacity. We have demonstrated that mice brain cholinergic system was altered by the effect of some porphyrinogenic agents. The aim of this work was to investigate how known porphyrinogenic drugs affect delta-Aminolevulinic acid synthetase (ALA-S), which is the response of heme oxygenase (HO) to this challenge and to evaluate if the xenobiotics studied develop stress oxidative in mice brain. HO activity was 50-70% induced after chronic Enflurane and Isoflurane anaesthesia, dietary Griseofulvin and starvation. An increase in mRNA HO expression was caused by chronic anaesthesia and Veronal treatments; instead allylisopropilacetamide (AIA) reduced mRNA expression. ALA-S activity was induced by acute administration of anaesthetics (89%), veronal (240%) and ethanol (80%), while ALA-S mRNA expression augmented by chronic administration of enflurane, AIA and veronal. Stress markers such as superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase activities and malondialdehyde and reduced glutathione levels showed different responses depending on the xenobiótic assayed. In conclusion, some of the drugs studied produced oxidative stress in brain that was confirmed through HO induction and this could be one of the factors leading to porphyric neuropathy. |
| title |
Heme oxygenase, aminolevulinate acid synthetase and the antioxidant system in the brain of mice treated with porphyrinogenic drugs |
| title_short |
Heme oxygenase, aminolevulinate acid synthetase and the antioxidant system in the brain of mice treated with porphyrinogenic drugs |
| title_full |
Heme oxygenase, aminolevulinate acid synthetase and the antioxidant system in the brain of mice treated with porphyrinogenic drugs |
| title_fullStr |
Heme oxygenase, aminolevulinate acid synthetase and the antioxidant system in the brain of mice treated with porphyrinogenic drugs |
| title_full_unstemmed |
Heme oxygenase, aminolevulinate acid synthetase and the antioxidant system in the brain of mice treated with porphyrinogenic drugs |
| title_sort |
heme oxygenase, aminolevulinate acid synthetase and the antioxidant system in the brain of mice treated with porphyrinogenic drugs |
| publishDate |
2005 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v51_n5_p487_Rodriguez http://hdl.handle.net/20.500.12110/paper_01455680_v51_n5_p487_Rodriguez |
| _version_ |
1768541885641523200 |