An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis.

Protein complexes present different degrees of stability. We have previously described a glycoprotein from Bacillus thuringiensis that appeared as a multimer unable to be dissociated by the usual SDS-containing sample buffers of pH 6.8. In order to dissociate the complex, a SDS-containing sample buf...

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Autor principal:	Tandecarz, Juana Sara
Publicado:	1998
Materias:	bacterial protein buffer dodecyl sulfate sodium article chemistry methodology micelle polyacrylamide gel electrophoresis Bacterial Proteins Buffers Electrophoresis, Polyacrylamide Gel Micelles Sodium Dodecyl Sulfate
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v44_n3_p557_GarciaPatrone http://hdl.handle.net/20.500.12110/paper_01455680_v44_n3_p557_GarciaPatrone
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01455680_v44_n3_p557_GarciaPatrone
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spelling	paper:paper_01455680_v44_n3_p557_GarciaPatrone2023-06-08T15:12:24Z An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis. Tandecarz, Juana Sara bacterial protein buffer dodecyl sulfate sodium article chemistry methodology micelle polyacrylamide gel electrophoresis Bacterial Proteins Buffers Electrophoresis, Polyacrylamide Gel Micelles Sodium Dodecyl Sulfate Protein complexes present different degrees of stability. We have previously described a glycoprotein from Bacillus thuringiensis that appeared as a multimer unable to be dissociated by the usual SDS-containing sample buffers of pH 6.8. In order to dissociate the complex, a SDS-containing sample buffer of pH 9 was described. In the present report three additional protein complexes with different degrees of stability and the effect of that dissociating sample buffer are described. The study of SDS critical micellar concentration values as a function of pH explains the improvement of dissociating properties at pH 9. Fil:Tandecarz, J.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v44_n3_p557_GarciaPatrone http://hdl.handle.net/20.500.12110/paper_01455680_v44_n3_p557_GarciaPatrone
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	bacterial protein buffer dodecyl sulfate sodium article chemistry methodology micelle polyacrylamide gel electrophoresis Bacterial Proteins Buffers Electrophoresis, Polyacrylamide Gel Micelles Sodium Dodecyl Sulfate
spellingShingle	bacterial protein buffer dodecyl sulfate sodium article chemistry methodology micelle polyacrylamide gel electrophoresis Bacterial Proteins Buffers Electrophoresis, Polyacrylamide Gel Micelles Sodium Dodecyl Sulfate Tandecarz, Juana Sara An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis.
topic_facet	bacterial protein buffer dodecyl sulfate sodium article chemistry methodology micelle polyacrylamide gel electrophoresis Bacterial Proteins Buffers Electrophoresis, Polyacrylamide Gel Micelles Sodium Dodecyl Sulfate
description	Protein complexes present different degrees of stability. We have previously described a glycoprotein from Bacillus thuringiensis that appeared as a multimer unable to be dissociated by the usual SDS-containing sample buffers of pH 6.8. In order to dissociate the complex, a SDS-containing sample buffer of pH 9 was described. In the present report three additional protein complexes with different degrees of stability and the effect of that dissociating sample buffer are described. The study of SDS critical micellar concentration values as a function of pH explains the improvement of dissociating properties at pH 9.
author	Tandecarz, Juana Sara
author_facet	Tandecarz, Juana Sara
author_sort	Tandecarz, Juana Sara
title	An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis.
title_short	An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis.
title_full	An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis.
title_fullStr	An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis.
title_full_unstemmed	An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis.
title_sort	improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis.
publishDate	1998
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v44_n3_p557_GarciaPatrone http://hdl.handle.net/20.500.12110/paper_01455680_v44_n3_p557_GarciaPatrone
work_keys_str_mv	AT tandecarzjuanasara animprovementinthedissociatingpropertiesofsodiumdodecylsulfatecontainingsamplebuffersusedinpolyacrylamidegelelectrophoresis AT tandecarzjuanasara improvementinthedissociatingpropertiesofsodiumdodecylsulfatecontainingsamplebuffersusedinpolyacrylamidegelelectrophoresis
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An improvement in the dissociating properties of sodium dodecyl sulfate-containing sample buffers used in polyacrylamide gel electrophoresis.

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