The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021.

Rhizobium meliloti associates symbiotically with alfalfa by forming root nodules in which the bacteria reduce atmospheric N2 into products useful to both organisms. Nod factors are signal molecules, lipooligosaccharides, produced by the bacteria that trigger nodule formation in the plant host. Nod R...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Semino, Carlos Eduardo, Dankert, Marcelo Alberto
Publicado:	1994
Materias:	lipid linked oligosaccharides lipid-linked oligosaccharides lipopolysaccharide alfalfa article bacterial gene biosynthesis carbohydrate analysis chemical structure chemistry genetics metabolism microbiology molecular genetics Sinorhizobium meliloti Carbohydrate Sequence Genes, Bacterial Lipopolysaccharides Medicago sativa Molecular Sequence Data Molecular Structure
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v40_n7_p1029_Semino http://hdl.handle.net/20.500.12110/paper_01455680_v40_n7_p1029_Semino
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01455680_v40_n7_p1029_Semino
record_format	dspace
spelling	paper:paper_01455680_v40_n7_p1029_Semino2023-06-08T15:12:19Z The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021. Semino, Carlos Eduardo Dankert, Marcelo Alberto lipid linked oligosaccharides lipid-linked oligosaccharides lipopolysaccharide alfalfa article bacterial gene biosynthesis carbohydrate analysis chemical structure chemistry genetics metabolism microbiology molecular genetics Sinorhizobium meliloti Carbohydrate Sequence Genes, Bacterial Lipopolysaccharides Medicago sativa Molecular Sequence Data Molecular Structure Sinorhizobium meliloti Rhizobium meliloti associates symbiotically with alfalfa by forming root nodules in which the bacteria reduce atmospheric N2 into products useful to both organisms. Nod factors are signal molecules, lipooligosaccharides, produced by the bacteria that trigger nodule formation in the plant host. Nod Rm-1 consists of a beta-1,4-N-acetyl glucosamine tetrasaccharide from which the N-acetyl group at the non reducing end is replaced by a fatty acid and the N-acetyl glucosamine at the reducing end is sulfated at position 6. By in vitro incubation of electroporated cells in the presence of [35S]PAPS or UDP-[14C]GlcNAc a labelled compound has been obtained with the properties of the in vivo produced Nod Rm-1 factor, as judged by HPLC, TLC and HPTLC techniques. The [14C]GlcNAc labelled compound has also hair root deformation activity on alfalfa plantlets indicating that a functional Nod Rm-1 factor has been synthesized in vitro. Fil:Semino, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dankert, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1994 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v40_n7_p1029_Semino http://hdl.handle.net/20.500.12110/paper_01455680_v40_n7_p1029_Semino
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	lipid linked oligosaccharides lipid-linked oligosaccharides lipopolysaccharide alfalfa article bacterial gene biosynthesis carbohydrate analysis chemical structure chemistry genetics metabolism microbiology molecular genetics Sinorhizobium meliloti Carbohydrate Sequence Genes, Bacterial Lipopolysaccharides Medicago sativa Molecular Sequence Data Molecular Structure Sinorhizobium meliloti
spellingShingle	lipid linked oligosaccharides lipid-linked oligosaccharides lipopolysaccharide alfalfa article bacterial gene biosynthesis carbohydrate analysis chemical structure chemistry genetics metabolism microbiology molecular genetics Sinorhizobium meliloti Carbohydrate Sequence Genes, Bacterial Lipopolysaccharides Medicago sativa Molecular Sequence Data Molecular Structure Sinorhizobium meliloti Semino, Carlos Eduardo Dankert, Marcelo Alberto The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021.
topic_facet	lipid linked oligosaccharides lipid-linked oligosaccharides lipopolysaccharide alfalfa article bacterial gene biosynthesis carbohydrate analysis chemical structure chemistry genetics metabolism microbiology molecular genetics Sinorhizobium meliloti Carbohydrate Sequence Genes, Bacterial Lipopolysaccharides Medicago sativa Molecular Sequence Data Molecular Structure Sinorhizobium meliloti
description	Rhizobium meliloti associates symbiotically with alfalfa by forming root nodules in which the bacteria reduce atmospheric N2 into products useful to both organisms. Nod factors are signal molecules, lipooligosaccharides, produced by the bacteria that trigger nodule formation in the plant host. Nod Rm-1 consists of a beta-1,4-N-acetyl glucosamine tetrasaccharide from which the N-acetyl group at the non reducing end is replaced by a fatty acid and the N-acetyl glucosamine at the reducing end is sulfated at position 6. By in vitro incubation of electroporated cells in the presence of [35S]PAPS or UDP-[14C]GlcNAc a labelled compound has been obtained with the properties of the in vivo produced Nod Rm-1 factor, as judged by HPLC, TLC and HPTLC techniques. The [14C]GlcNAc labelled compound has also hair root deformation activity on alfalfa plantlets indicating that a functional Nod Rm-1 factor has been synthesized in vitro.
author	Semino, Carlos Eduardo Dankert, Marcelo Alberto
author_facet	Semino, Carlos Eduardo Dankert, Marcelo Alberto
author_sort	Semino, Carlos Eduardo
title	The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021.
title_short	The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021.
title_full	The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021.
title_fullStr	The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021.
title_full_unstemmed	The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021.
title_sort	in vitro biosynthesis of functional nodulation factors (nod rm) produced by rhizobium meliloti 1021.
publishDate	1994
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v40_n7_p1029_Semino http://hdl.handle.net/20.500.12110/paper_01455680_v40_n7_p1029_Semino
work_keys_str_mv	AT seminocarloseduardo theinvitrobiosynthesisoffunctionalnodulationfactorsnodrmproducedbyrhizobiummeliloti1021 AT dankertmarceloalberto theinvitrobiosynthesisoffunctionalnodulationfactorsnodrmproducedbyrhizobiummeliloti1021 AT seminocarloseduardo invitrobiosynthesisoffunctionalnodulationfactorsnodrmproducedbyrhizobiummeliloti1021 AT dankertmarceloalberto invitrobiosynthesisoffunctionalnodulationfactorsnodrmproducedbyrhizobiummeliloti1021
_version_	1768543272274231296

The in vitro biosynthesis of functional nodulation factors (Nod Rm) produced by Rhizobium meliloti 1021.

Ejemplares similares